Team:Gifu/Notebook

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<a class="tab-gray" href="https://2014.igem.org/Team:Gifu/Protocol">Protocols</a>
<a class="tab-gray" href="https://2014.igem.org/Team:Gifu/Protocol">Protocols</a>
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<a class="tab-white" href="https://2014.igem.org/Team:Gifu/Notebook" >Calender</a></div>
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<a class="tab-white" href="https://2014.igem.org/Team:Gifu/Notebook" >Calender</a></div>
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<h1  style="font-family:Comic Sans MS;font-size:x-large">Calender</h1>
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<h1  style="font-family:Comic Sans MS;font-size:x-large" name="calender">Calender</h1>
<p class="abc"> Click on a date to see our research activities on that day. </p>
<p class="abc"> Click on a date to see our research activities on that day. </p>

Revision as of 03:54, 3 September 2014


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head home team project note result more



Calender

Click on a date to see our research activities on that day.

>
AugustSeptember
MonTueWedThrFriSatSun  MonTueWedThrFriSatSun
123   1234567
45678910   891011121314
11121314151617   15161718192021
18192021222324   22232425262728
25262728293031   2930

August

August 11th

Restriction Enzyme Digestion

samplerestriction enzymes
His-RFP(with stop codon)SpeI,EcoRI
5'-intron MSpeI,EcoRI
Lac-3'intron-RBSSpeI,EcoRI
His-RFP(without stop codon)XbaI,EcoRI
RBS-AmpSpeI,EcoRI

Ligation

  1. EcoRI-His-RFP(with stop codon)-SpeI + XbaI-DT-Cm-EcoRI
  2. EcoRI-5'intron M-SpeI + XbaI-DT-Cm-EcoRI
  3. EcoRI-Lac-3'intron-RBS-SpeI + XbaI-His-RFP(without stop codon)-5'intron-DT-Cm-EcoRI
  4. EcoRI-His-RFP(without stop codon)-SpeI + SpeI-Amp-EcoRI

Transformation

  1. -His-RFP(with stop codon)-DT-Cm-
  2. -5'intron M-DT-Cm-
  3. -Lac-3'intron-RBS-His-RFP(without stop codon)-5'intron-DT-Cm-
  4. -His-RFP(without stop codon)-Amp-

Densitometry

sampleconcentration(ng/µL)
His-RFP(with stop codon) EcoRI-SpeI162.6
5'intron M EcoRI-SpeI45.4
Lac-3'intron-RBS EcoRI-SpeI120.9
His-RFP(without stop codon) EcoRI-SpeI160.0
His-RFP(without stop codon)-5'intron-DT-Cm XbaI-EcoRI 174.0
Amp EcoRI-SpeI101.8

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August 12th

Colony PCR

  1. His-RFP(with stop codon)-DT-Cm
  2. 5'intron M-DT-Cm
  3. Lac-3'intron-RBS-His-RFP(without stop codon)-5'intron-DT-Cm:[1]turning red
  4. Lac-3'intron-RBS-His-RFP(without stop codon)-5'intron-DT-Cm:[2]turning pink
  5. Lac-3'intron-RBS-His-RFP(without stop codon)-5'intron-DT-Cm:[3]turning white
  6. His-RFP(without stop codon)-Amp

Electrophoresis

  1. His-RFP(with stop codon)-DT
  2. His-RFP
  3. 5'intron M-DT
  4. 5'intron M
  5. Lac-3'intron-RBS-His-RFP(without stop codon)-5'intron-DT:[1]
  6. Lac-3'intron-RBS-His-RFP(without stop codon)-5'intron-DT:[2]
  7. Lac-3'intron-RBS-His-RFP(without stop codon)-5'intron-DT:[3]
  8. His-RFP(without stop codon)-5'intron-DT
  9. His-RFP(without stop codon)

Liquid Culture

  1. His-RFP(with stop codon)-DT
  2. 5'intron M-DT
  3. Lac-3'intron-RBS-His-RFP(without stop codon)-5'intron-DT:[1]
  4. Lac-3'intron-RBS-His-RFP(without stop codon)-5'intron-DT:[3]
  5. His-RFP(without stop codon)

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August 13th

Miniprep

PCR

By use of KAPA HiFi DNA Polymerase
  1. His-RFP(with stop codon)-DT-Cm
  2. 5'intron M-DT-Cm
By use of Taq DNA Polymerase
  1. Lac-3'intron-RBS-His-RFP(without stop codon)-5'intron-DT-Cm:[1]turning red
  2. Lac-3'intron-RBS-His-RFP(without stop codon)-5'intron-DT-Cm:[3]turning white

Electrophoresis

  1. His-RFP(with stop codon)-DT-Cm
  2. 5'intron M-DT-Cm
  3. DNA Ladder Markers DNA Ladder One(Broad Range)
  4. Lac-3'intron-RBS-His-RFP(without stop codon)-5'intron-DT-Cm:[1]turning red
  5. Lac-3'intron-RBS-His-RFP(without stop codon)-5'intron-DT-Cm:[3]turning white

Restriction Enzyme Digestion

samplerestriction enzymes
His-RFP(with stop codon)-DT-CmEcoRI,XbaI
5'intron M-DT-CmEcoRI,XbaI

Densitometry

sampleconcentration(ng/µL)
EcoRI-Lac-RBS-SpeI65.0
XbaI-His-RFP(with stop codon)-DT-Cm-EcoRI188.8
EcoRI-His-RFP(with stop codon)-SpeI160.0
EcoRI-His-RFP(without stop codon)-SpeI160.0
XbaI-5'intron M-DT-Cm-EcoRI167.8

Ligation

  1. EcoRI-Lac-RBS-SpeI + XbaI-His-RFP(with stop codon)-DT-Cm-EcoRI
  2. EcoRI-His-RFP(with stop codon)-SpeI + XbaI-5'intron M-DT-Cm-EcoRI
  3. EcoRI-His-RFP(without stop codon)-SpeI + XbaI-5'intron M-DT-Cm-EcoRI

Transformation

  1. -Lac-RBS-His-RFP(with stop codon)-DT-Cm-
  2. -His-RFP(with stop codon)-5'intron M-DT-Cm-
  3. -His-RFP(without stop codon)-5'intron M-DT-Cm-

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August 16th

Liquid Culture

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August 18th

Plasmid Extraction

  1. -Lac-RBS-His-RFP(with stop codon)-DT-Cm-
  2. -His-RFP(with stop codon)-5'intron M-DT-Cm-
  3. -His-RFP(without stop codon)-5'intron M-DT-Cm-

Plasmid Purification

  1. -5'intron M-His-RFP(with stop codon)-
  2. -5'intron M-His-RFP(without stop codon)-

Miniprep

Colony PCR

Densitometry

Ligation

Transformation

Analysis of DNA sequences

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August 19th

Colony PCR

Electrophoresis

Shaking Culture

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August 20th

Colony PCR

Same as August 19th Colony PCR.

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August 21th

Reagent Preparation

Protein Extraction

SDS-PAGE

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August 22th

Prorein Purification by use of Ni-NTA

SDS-PAGE

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August 23th

Prorein Purification by use of Ni-NTA

SDS-PAGE

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August 24th

Extraction of plasmid device

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August 25th

Extraction of plasmid device

SDS-PAGE

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August 26th

PCR

Restriction Enzyme Digestion

Densitometry

Ligation

Transformation

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August 27th

Colony PCR

Shaking Culture

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August 28th

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Retrieved from "http://2014.igem.org/Team:Gifu/Notebook"