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{{Team:HokkaidoU_Japan/Common/CSS}} {{Team:HokkaidoU_Japan/Notebook/CSS}} <div class="wrapper"> <div id="hokkaidou-contents"> <div class="workflow-container"> <ul class="steps"> <li class="step start"> <div class="step-tile"> <h2 class="step-title>Start</h2> </div> </li> <li class="step"> <div class="step-tile"> <h2 class="step-title> Transformation </h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-triale"> <li> <time> Fri Aug 22 </time> </li> </ul> <div class="step-recipi"> Ptrc-stem-asB0034-stem on pHN1257&Ptet-B0034-mRFP-dt on pSB6A1 on ice 30min each of 2.5&micro;L DNA to DH5&alpha; 42C 30sec on ice 2min </div> </div> </div> </li> <li class="step"> <div class="step-tile"> <h2 class="step-title> Colony PCR </h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-triale"> <li> <time> Sat Aug 23 </time> </li> </ul> <div class="step-recipi"> Ptrc-stem-asB0034-stem(pHN1257)&Ptet-B0034-mRFP-dT(pSB6A1) pHN1257_up_F Tm:60.1 asB0034-R Tm:68.7 </div> </div> </div> </li> <li class="step"> <div class="step-tile"> <h2 class="step-title> Preculture </h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-triale"> <li> <time> Mon Aug 25 </time> </li> </ul> <div class="step-recipi"> Ptrc-stem-asB0034-stem(pHN1257)&Ptet-B0034-mRFP-dT(pSB6A1) LB 2 mL for 3hours Turbidity was controlled 0.1 </div> </div> </div> </li> <li class="step"> <div class="step-tile"> <h2 class="step-title> Culture </h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-triale"> <li> <time> Thu Aug 26 </time> </li> </ul> <div class="step-recipi"> LB 2 mL IPTG 20 &micro; or not for 11 hours, 13hours, 15hours, 17 hours </div> </div> </div> </li> <li class="step"> <div class="step-tile"> <h2 class="step-title> Assay </h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-triale"> <li> <time> Thu Aug 26 </time> </li> </ul> <div class="step-recipi"> fluoresence imageing 514 nm 610 filter </div> </div> </div> </li> <li class="step complete"> <div class="step-tile"><h2 class="step-title>Complete!</h2></div> </li> </ul> </div> </html>

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