Team:Evry/Notebook/Sensing/PCBs/08-27-2014

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<u><b>Sensor construction bphR2/PbpR1</b></u>
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<br/>Some colonies had grown after the transformation of bphR2 in pSB1C3: 
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<br/><img src="https://static.igem.org/mediawiki/2014/2/2e/Igemevry2014_BphR2_in_pSB1C3.JPG" width=300px alt="image not found" />
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Products PCR from 26th august has been migrated : 
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<br/><br/><img src="https://static.igem.org/mediawiki/2014/e/e6/Igemevry2014_Gel_pcr_clean_up.jpg" width=500px alt="image not found" />
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<br/>Expected for the terminator, we have bands at the good tail. So for the terminator, an other PCR has been made but by using the one taq polymerase instead of Q5 DNA polymerase and we obtained the good band:
<br/><img src="https://static.igem.org/mediawiki/2014/7/73/Igemevry2014_Terminator_one_taq.png" width=500px alt="image not found" />
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<span class="cd-date">Aug 27</span> 
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