Team:Bordeaux/NotebookAugust26

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<h1>26<sup>th</sup> August </h1>
<div class="type-exp"> Molecular biology</div>

<br>

<ul class="day-detail">
<li>Results of transformation
<div style="margin-left:35px;">
pSB6A1 : 193 CFU<br>
ELP-ELP 1: 1776 CFU<br>
ELP-ELP 2: 1104 CFU<br>
ELP-SLP: 2016 CFU<br>
ELP-SLP: 2400 CFU<br>
ELP-RLP: 1876 CFU<br>
ELP-RLP: 2368 CFU<br>
SLP-SLP: 93 CFU<br>
SLP-SLP: 91 CFU<br>
SLP-RLP: 10 CFU<br>
SLP-RLP: 248 CFU<br>
RLP-RLP: 1872 CFU<br>
RLP-RLP: 1424 CFU<br>
</div>
</li>
<br>
<li>
Digestion pSB1C3
<table>
  <tr> 
    <td>Buffer (cutsmart) 	</td>
    <td>5μl</td>
  </tr>
  <tr>
    <td>Xba1	</td>
    <td>0,5μl</td> 
  </tr>
  <tr> 
    <td>Spe1	</td>
    <td>0,5μl</td>
  </tr>
  <tr>
    <td>DNA	</td>
    <td>10μl</td> 
  </tr>
  <tr>
    <td>H2O	</td>
    <td>qsp 50μl</td> 
  </tr>
</table>
<br>
=> 1h at 37°C and gel 1% (30min, 100V)
</li><br>
<li>
Miniprep pSB1C3<br>
Sample 1: 224,2ng/μl<br>
Sample 2: 313,4ng/μl<<br>
Sample 3: 269,9ng/μl<br>
</li>
</ul>
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