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This checklist contains everything you will need for setting up transformation **** DNA - A [[helper plasmid]] for agrobacterium mediated transformation - Your [[T-DNA]] plasmid for agrobacterium mediated transformation [see the MoClo plate]. **** [[Bacteria]] - Competent E-Coli (chemically/electro) - [[Electrocompetent agrobacteria]] **** Marchantia - [[Marchantia sporangia]] OR - Male and female Marchantia polymorpha [from the garden] **** Misc - Gamborg's B5 salt mix - LB/SOC for bacterial transformation and growth - 100mM Acetosyringone **** Equipment - Ventilated cupboard/room/box under full illumination [larger space = more transformations]

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