Team:Heidelberg/Project/LOV

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title=in LOV
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subtitle= controlling inteins with light
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titles={{:Team:Heidelberg/templates/title|Introduction}}{{:Team:Heidelberg/templates/title|Experimental procedures|Cloning_and_Methods}}{{:Team:Heidelberg/templates/title|Results}}{{:Team:Heidelberg/templates/title|Discussion}}{{:Team:Heidelberg/templates/title|References}}
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abstract= <p>Light-dependent regulation represents a promising way to control biological processes and it offers an unmatched spatio-temporal resolution and only minimal toxic effects. To complete our toolbox we therefore came up with an advanced system to induce intein splicing via blue-light illumination. Previously, attempts have been made to engineer light inducible split intein pairs using photoinduced complementation systems, these methods however only allow for light induction of inteins with low affinity and therefore low splicing activity.</p>
<p>inLOV (<b>in</b>teins controlled by As <b>LOV</b>2) uses small split inteins photocaged in the <i> Avena Sativa</i> LOV2 domain to inhibit the binding of inteins in non illuminated dark-state and to only facilitate protein splicing upon induction with light of &#955;=470&thinsp;nm.</p>
<p>The system is fully compatible with our toolbox parts and might in future become one of the most efficient and universally applicable ways of photo inducing post-translational modifications <i>in vivo</i>.</p>
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