Team:WPI-Worcester/Our-Construct

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<p  style="text-align:center;"><a href="https://2014.igem.org/Team:WPI-Worcester"><img src="https://static.igem.org/mediawiki/2014/3/3f/Wpibanner.png" width="800px" length="200px"></a></p>

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	<li> <a href="#"><center><img src="https://static.igem.org/mediawiki/2014/6/64/WPI_Team_Link.png"/></center> <p>Team</p></a>
		<ul>			
			<li><a href="https://2014.igem.org/Team:WPI-Worcester/Team">Bios</a></li>
			<li><a href="https://2014.igem.org/Team:WPI-Worcester/Team-Gallery">Team Gallery</a></li>
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	<li><a href="#"><center><img src="https://static.igem.org/mediawiki/2014/4/41/WPI_Project_Link.png"/></center><p>Project</p></a> 
		<ul> 
	    <li><a href="https://2014.igem.org/Team:WPI-Worcester/Overview">Project Overview and Abstract</a></li> 
            <li><a href="https://2014.igem.org/Team:WPI-Worcester/Background">Background</a></li>
            <li><a href="https://2014.igem.org/Team:WPI-Worcester/Motivation">Motivation</a></li>
	    <li><a href="https://2014.igem.org/Team:WPI-Worcester/Future-Applications">Future Applications</a></li>

		</ul> 
	</li> 
	
	<li><a href="#"><center><img src="https://static.igem.org/mediawiki/2014/f/f1/Accomplishments_RealLink.png"/></center><p>Accomplishments</p></a> 
		<ul> 
			<li><a href="https://2014.igem.org/Team:WPI-Worcester/Our-Construct">Our Construct</a></li>
			<li><a href="https://2014.igem.org/Team:WPI-Worcester/Proof-of-Principle">Proof of Principle</a></li>
			<li><a href="https://2014.igem.org/Team:WPI-Worcester/ATF1">Biobrick Characterization</a></li>
			<li><a href="https://2014.igem.org/Team:WPI-Worcester/Biobricks">Biobricks</a></li> 
			<li><a href="https://2014.igem.org/Team:WPI-Worcester/Medal-Fulfillment">Medal Fulfillment</a></li>

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	<li><a href="#"><center><img src="https://static.igem.org/mediawiki/2014/7/7e/WPI_Notes_Link.png"/></center><p>Notebook</p></a>
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			<li><a href="https://2014.igem.org/Team:WPI-Worcester/Notebook">Weekly Log</a></li>
			<li><a href="https://2014.igem.org/Team:WPI-Worcester/Protocols">Protocols</a></li>
		
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	<li><a href="#"><center><img src="https://static.igem.org/mediawiki/2014/d/d4/WPI_Safety_Link.png"/></center><p>Practices</p></a> 
		<ul>
            <li><a href="https://2014.igem.org/Team:WPI-Worcester/Survey">Survey</a></li>
            <li><a href="https://2014.igem.org/Team:WPI-Worcester/Outreach">Outreach</a></li>
 		<li><a href="https://2014.igem.org/Team:WPI-Worcester/Collaborations">Collaborations</a></li>
		<li><a href="https://2014.igem.org/Team:WPI-Worcester/Interlab">Interlab Study</a></li>
  		<li><a href="https://2014.igem.org/Team:WPI-Worcester/Safety">Safety</a></li>
            <li><a href="https://2014.igem.org/Team:WPI-Worcester/Beyond-the-Bench">Beyond the Bench</a></li>

        </ul>


	<li><a href="#"><center><img src="https://static.igem.org/mediawiki/2014/e/e9/Acknowledgements_Link.png"/></center><p>Acknowledgements</p></a>
		<ul>
			<li><a href="https://2014.igem.org/Team:WPI-Worcester/Sponsors">Sponsors</a></li>
			<li><a href="https://2014.igem.org/Team:WPI-Worcester/Attributions">Attributions</a></li>
		
		</ul>
	</li>
	</li>
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</br>
<h4>Our Construct</h4><p><center><img src="https://static.igem.org/mediawiki/2014/thumb/e/e0/WPI_OurConstruct.png/800px-WPI_OurConstruct.png"/></center></p><p>The construct developed in this project is a combination of the N-terminal domain of BclA and the CAEV p28 antigen. The sequence encoding the N-terminal domain of BclA is ligated to the sequence for the CAEV p28 antigen. Both sequences were ordered as codon-optimized oligos, so that <i>B. anthracis</i> and CAEV would not have to be worked with directly. This plasmid is then combined with a ribosome binding site, a constitutive promoter, and a double terminator in order to make a complete construct. The resulting plasmid allows the <i>E. coli</i> it is transformed into to express the CAEV p28 antigen connected to the N-terminal domain of BclA, which is anchored in the outer surface of the plasma membrane. This plasmid is transformed into competent <i>E. coli</i>,  which then express CAEV on their membrane surface, thanks to the anchoring of BclA’s N-terminal domain. 
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