Team:Yale/Parts

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		<h1 style="margin-top:22px; font-size:50px;">Parts</h1>	
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		<h1>Submitted Parts</h1>
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<div class="well"><p>
The collection consists of the BioBrick mussel foot protein (MFP) 1-5-1 sequence (combination of  Mytilus galloprovincialis Foot Protein 5 (Mgfp-5) and Mytilus Edulis Foot Protein1 (Mefp-1)). The second BioBrick is the MFP with superfolder Green Fluorescence Protein (sfGFP). The third BioBrick is the MFP with our antimicrobial peptide of interest, LL-37. Finally, the fourth BioBrick is our entire construct of our antimicrobial peptide: 2XStrep_Flagtag_LL-37_Mussel Foot Protein_sfGFP. All biobricks are in the pSB1C3 plasmid.
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		<h1>Full construct: <a href= "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1396000">BBa_K1396000</a></h1>
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The part is an coding sequence for an anti-microbial peptides linked to a mussel-foot protein-linked to superfolder GFP for localization. The mussel foot protein will anneal to surfaces as a wet glue and the antimicrobial domain is designed to interact with microbial membranes and interfere with membrane stability. In order to use this part you can produce it in a TAG recoded organism simultaneously expressing a Tyrosine supressor or L-DOPA orthogonal translational system. In order to purify you can use the 2X Strep tag and strep column and later cleave with enterokinase to remove the sequence supressing LL-37 antimicrobial action.</p>

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<img src="https://static.igem.org/mediawiki/2014/b/b7/Full_Construct.jpg">
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<tr><td colspan="2"><h2>LL37-MFP: <a href= "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1396001">BBa_K1396001</a></h2>
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<p>
The part is an coding sequence for an anti-microbial peptides linked to a mussel-foot protein. The mussel foot protein will anneal to surfaces as a wet glue and the antimicrobial domain is designed to interact with microbial membranes and interfere with membrane stability. In order to use this part you can produce it in a TAG recoded organism simultaneously expressing a Tyrosine suppressor or L-DOPA orthogonal translational system. In order to purify you can use the 2X Strep tag and strep column and later cleave with enterokinase to remove the sequence suppressing LL-37 antimicrobial action.
This is an improvement on the Utah State biobrick <a href= "http://parts.igem.org/Part:BBa_K1162006">BBa_K1162006 </a> which consists of only the LL-37 peptide.</p>

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<img src="https://static.igem.org/mediawiki/2014/f/f4/LL37_FP151.jpg">
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<tr><td colspan="2"><h2>MFP-sfGFP: <a href= "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1396002">BBa_K1396002</a></h2>
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The part is an coding sequence for an anti-microbial peptides linked to a mussel-foot protein-linked to superfolder GFP for localization. The mussel foot protein will anneal to surfaces as a wet glue and superfolder GFP will allow for florescence imaging and localization. In order to use this part you can produce it in a TAG recoded organism simultaneously expressing a Tyrosine supressor or L-DOPA orthogonal translational system. In order to purify you can use the 2X Strep tag and strep column and later cleave with enterokinase to remove the sequence supressing LL-37 antimicrobial action.</p>

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<img src="https://static.igem.org/mediawiki/2014/5/5b/FP151_GFP.jpg">
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<tr><td colspan="2"><h2>Mussel Foot Protein 1-5-1: <a href= "https://static.igem.org/mediawiki/2014/0/04/FP151.jpg">BBa_K1396003</a></h2>
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Recoded and codon optimized coding sequence for the mussel foot protein 151. TAG is recoded. In order to produce the protein co-express in cells contain either an L-DOPA orthogonal translation system or a Tyrosine suppressor.</p>

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<img src="https://static.igem.org/mediawiki/2014/0/04/FP151.jpg">
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