Team:TU Delft-Leiden/Project/Life science/curli/cloning/GoldenGate

{{CSS/Delft2014_main}}
{{CSS/960_12_col}}
{{:Team:TU_Delft-Leiden/Templates/Start}}

<html>
              
 <!--PAGE CONTENT-----------------> 
<h2> Curli Module - Cloning - Golden Gate Assembly</h2>
<p>
To construct the BioBricks <a href="http://parts.igem.org/Part:BBa_K1316013">BBa_K1316013 </a> and <a href="http://parts.igem.org/Part:BBa_K1316014">BBa_K1316014 </a>, encoding a rhamnose promoter and the coding sequences of <i>csgB</i> and <i>csgA</i> or <i>csgA-HIS</i>, we ended up having 3 pieces of DNA to be cloned into pSB1C3. To work efficiently, we used the LINK Golden Gate Assembly to construct our BioBricks (see figure 1). 
</p>
<p>

<figure>
<center><img style="margin-left: -10%;" src="https://static.igem.org/mediawiki/2014/c/c6/TUDELFT2014_goldengateCC.PNG" width="120%"> </center>
<figcaption>
Figure 1. <b>Golden Gate Assembly of <a href="http://parts.igem.org/Part:BBa_K1316013">BBa_K1316013 </a> and <a href="http://parts.igem.org/Part:BBa_K1316014">BBa_K1316014 </a>.</b> The BioBricks both encode a rhamnose promoter (red rectangle) and the coding sequences of <i>csgB</i> and <i>csgA</i> or <i>csgA-HIS</i>, indicated with grey arrows. The beam beneath the arrows visualizes the regions and relative sizes of the 3 DNA pieces that were ligated into pSB1C3. 
</figcaption>
</figure>
</p>
</html>
{{:Team:TU_Delft-Leiden/Templates/End}}