Team:Kyoto/Material and Method

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         <li><a id="kyoto-side-title" href="#title">Material & Method</a></li>
         <li><a href="#parts">Parts</a></li>
         <li><a href="#primers">Primers</a></li>
         <li><a href="#protocol">Protocol</a>
            <ul>
               <li><a href="#pcr">PCR</a></li>
               <li><a href="#mutation_pcr">Mutation PCR</a></li>
               <li><a href="#rna_extraction">RNA Extraction</a></li>
               <li><a href="#competent_cells">Making Competent Cells</a></li>
               <li><a href="#miniprep">Miniprep</a></li>
               <li><a href="#ethanol_precipitation">Ethanol Precipitation</a></li>
               <li><a href="#electrophoresis">Electrophoresis</a></li>
               <li><a href="#gel_extraction">Gel Extraction and PCR Purification</a></li>
               <li><a href="#ligation">Ligation</a></li>
               <li><a href="#digestion">Restriction Enzyme Digestion</a></li>
               <li><a href="#media">Media</a></li>
               <li><a href="#buffer_tb">Buffer TB</a></li>
               <li><a href="#solubilization_of_antibiotics">Solubilization of Antibiotics</a></li>
               <li><a href="#transformation">Transformation</a></li>
               <li><a href="#sequence">Sequence</a></li>
               <li><a href="#qrt-pcr">qRT-PCR</a></li>
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      <h1 id="kyoto-title">Material & Method</h1>
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      <h2>Parts</h2>
      <p>If you see our team's parts in part.igem.org, <a href="http://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=Kyoto">Click Here</a>.</p>
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<groupparts>iGEM014 Kyoto</groupparts>
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      <a name="primers" class="kyoto-jump"></a>
      <h2>Primers</h2>
      <h3>Magnetosome</h3>
      <table class="kyoto-primers">
         <tr><th colspan="2">mamL</th></tr>
         <tr><td>mamL-AMB-F</td><td>GCAGTATTGGCGTCAGCTTGGAG</td></tr>
         <tr><td>mamL-AMB-R</td><td>AACCGCTCTTCCTCATCCTTACTCAC</td></tr>
         <tr><td>Xba-RBS-mamL-F</td><td>GCTCTAGAAAAGAGGAGAAAAGTGGCATGGTAAGATTGATCG</td><tr>
         <tr><td>Pst-Spe-His-mamL-R</td><td>AACTGCAGCGGCCGCTACTAGTATCAGTGGTGATGGTGATGATGGCGCTTGATGACGATGCTC</td><tr>
         <tr><th colspan="2">mamQ</th></tr>
         <tr><td>mamQ-AMB-F</td><td>CCGCATTTCAAGAAGGCCAATGAAC</td></tr>
         <tr><td>mamQ-AMB-R</td><td>AACGAATCAGGCCCAGCACC</td></tr>
         <tr><td>Xba-RBS-mamQ-F</td><td>GCTCTAGAAAAGAGGAGAAAGCGGATATGGCATTAGGCG</td></tr>
         <tr><td>Pst-Spe-His-mamQ-R</td><td>AACTGCAGCGGCCGCTACTAGTATCAGTGGTGATGGTGATGATGTTTCTTGATGTCCTGCGCATG</td></tr>
         <tr><th colspan="2">mamB</th></tr>
         <tr><td>mamB-AMB-F</td><td>GGGACGATCAAGGCGAAGAAGG</td></tr>
         <tr><td>mamB-AMB-R</td><td>ACGGCTCAACATACGCTCTGG</td></tr>
         <tr><td>Eco-Xba-RBS-mamB-F</td><td>CGGAATTCGCGGCCGCTTCTAGAGAAAGAGGAGAAAGAACCGATGAAGTTCGAAAATTGCA</td></tr>
         <tr><td>Spe-mamB-R</td><td>GGACTAGTGGCTCAACATACGCTCTGG</td></tr>
         <tr><td>Eco-Pcon-Xba-RBS-mamB-F</td><td style="font-size: 0.8em;">CGGAATTCTTGACGGCTAGCTCAGTCCTAGGTACAGTGCTAGCTCTAGAAAAGAGGAGAAAGAACCGATGAAGTTCGAAAATTGCA</td></tr>
         <tr><td>Spe-His-mamB-R</td><td>GGACTAGTCTAGTGGTGATGGTGATGATGGACGAACAGGCGGATGTCG</td></tr>
         <tr><td>mutation-mamB-F</td><td>CTGCAAACCGCCTGGCAGGTGGCCA</td></tr>
         <tr><td>mutation-mamB-R</td><td>CCAGGCGGTTTGCAGCAATTCCGTATCC</td></tr>
         <tr><th colspan="2">Sequence</th></tr>
         <tr><td>pLQB-seq-1</td><td>AGCTGCTTGCCCAGTTG</td></tr>
         <tr><td>pQLB-seq-2</td><td>TTTGATGGCGGTGGTGG</td></tr>
         <tr><td>pQLB-seq-3</td><td>TCGGCTACGGCAACATC</td></tr>
         <tr><td>pLQB-seq-4</td><td>AGTTCGACATCCGCCTG</td></tr>
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         <h3>DMS</h3>
         <table class="kyoto-primers">
         <tr><th colspan="2">AT</th></tr>
         <tr><td></td><td></td></tr>
         <tr><th colspan="2">REDOX</th></tr>
         <tr><th colspan="2">SAMmt</th></tr>
         <tr><th colspan="2">DECARB</th></tr>
         <tr><th colspan="2">DiDECARB</th></tr>
         <tr><th colspan="2">dddD</th></tr>
         <tr><td>F dddD クローニング前PCR用　66</td><td>GTT CAA GCA GGC AGG CAA G</td></tr>
         <tr><td>R dddD クローニング前PCR用　65</td><td>AGC AAC GGC ACA TAT TCG AG</td></tr>
         <tr><td>F Pre-dddD　クローニング　62</td><td>CTC GAA TTC GCG GCC GCT TCT AGA TGC AGA ATC GCC CGC TTT CC</td></tr>
         <tr><td>Rv SpeⅠ-dddD cloning　ver3 68</td><td>CTA CTA GTA CGA GGC GTG GTT GCT GGT CC</td></tr>
         <tr><td>Fw dddD PstⅠ mutation 78.7</td><td>TGC AAC GTG AAA GCG AAG GCG AGA TCG AC</td></tr>
         <tr><td>Rv dddD PstⅠ mutation 78.4</td><td>CGC TTT CAC GTT GCA GGC CCG CAT C</td></tr>
      </table>


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      <h2>Protocol</h2>
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