LOCUS Exported 4460 bp ds-DNA circular SYN 17-OCT-2014 DEFINITION . ACCESSION . VERSION . KEYWORDS piG0096_pluxRRR12y-sfGFP-maxRBS-luxI SOURCE synthetic DNA construct ORGANISM recombinant plasmid REFERENCE 1 (bases 1 to 4460) AUTHORS Daniel Gerngross TITLE Direct Submission JOURNAL Exported 17 Oct 2014 from SnapGene 2.5.0 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4460 /organism="recombinant plasmid" /mol_type="other DNA" terminator 1..126 /note="T0" terminator 27..121 /note="lambda t0 terminator" /note="transcription terminator from phage lambda" misc_feature 127..196 /note="R2oDNA spacer" /note="R2oDNA 70bp spacer 13" terminator 203..230 /note="T7Te" /note="phage T7 early transcription terminator" misc_feature complement(238..308) /note="taR12y" promoter complement(309..363) /note="lux rR (R0062)" misc_feature 364..433 /note="spacer (B0040)" promoter 434..488 /note="lux rR (R0062)" 3'UTR 489..540 /note="crR12y" RBS 523..534 /note="RBS (B0034)" CDS 541..1266 /codon_start=1 /note="sfGFP" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE FVTAAGITHGMDELYKRP*" RBS 1287..1291 /note="RBS (B0034)" CDS 1299..1883 /codon_start=1 /note="luxI (C0161)" /translation="MTIMIKKSDFLAIPSEEYKGILSLRYQVFKQRLEWDLVVENNLES DEYDNSNAEYIYACDDTENVSGCWRLLPTTGDYMLKSVFPELLGQQSAPKDPNIVELSR FAVGKNSSKINNSASEITMKLFEAIYKHAVSQGITEYVTVTSTAIERFLKRIKVPCHRI GDKEIHVLGDTKSVVLSMPINEQFKKAVLN*" terminator 1895..1981 /gene="Escherichia coli rrnB" /note="T1" /note="transcription terminator T1 from the E. coli rrnB gene" terminator 1975..2002 /note="T1" primer_bind 2009..2028 /note="AB179" primer_bind 2194..2213 /note="AB5" rep_origin 2347..3174 /direction=RIGHT /note="p15A" rep_origin 2595..2597 /direction=RIGHT /note="P15 ori from pACYC184" rep_origin complement(2598..3142) /direction=LEFT /note="p15A ori" /note="Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells that contain a second plasmid with the ColE1 origin." primer_bind 3246..3265 /note="AB180" oriT 3276..3384 /note="incP origin of transfer" misc_feature 3356 /note="C->T no real mutation, just for testing" CDS complement(3540..4355) /note="KamR" ORIGIN 1 aaatcgtaat tattggggac ccctggattc tcaccaataa aaaacgcccg gcggcaaccg 61 agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt 121 ccaagatatt cgtcacagta gttcaacctc agatacagaa accgtagatt gggattcagg 181 aagttaccct attgtttata aacgcagaaa ggcccacccg aaggtgagcc agtgtgatct 241 agagatatat ggtagtagta agttaatttt cattaaccac cactaccaat cacctcctgg 301 atttagcttt tattcgacta taacaaacca ttttcttgcg taaacctgta cgatcctaca 361 ggtaggttct gttaagtaac tgaacccaat gtcgttagtg acgcttacct cttaagaggt 421 cactgaccta acaacctgta ggatcgtaca ggtttacgca agaaaatggt ttgttatagt 481 cgaataaaga attctaccat tcacctcttg gatttagcta ttaaagagga gaaaggtacc 541 atgagcaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt 601 gatgttaatg ggcacaaatt ttctgtccgt ggagagggtg aaggtgatgc tacaaacgga 661 aaactcaccc ttaaatttat ttgcactact ggaaaactac ctgttccgtg gccaacactt 721 gtcactactc tgacctatgg tgttcaatgc ttttcccgtt atccggatca catgaaacgg 781 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaacgcac tatatctttc 841 aaagatgacg ggacctacaa gacgcgtgct gaagtcaagt ttgaaggtga tacccttgtt 901 aatcgtatcg agttaaaggg tattgatttt aaagaagatg gaaacattct tggacacaaa 961 ctcgagtaca actttaactc acacaatgta tacatcacgg cagacaaaca aaagaatgga 1021 atcaaagcta acttcaaaat tcgccacaac gttgaagatg gttccgttca actagcagac 1081 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac 1141 ctgtcgacac aatctgtcct ttcgaaagat cccaacgaaa agcgtgacca catggtcctt 1201 cttgagtttg taactgctgc tgggattaca catggcatgg atgagctcta caaaaggcct 1261 taataaagcg aaacacgcat attttaagga ggtactacat gactataatg ataaaaaaat 1321 cggatttttt ggcaattcca tcggaggagt ataaaggtat tctaagtctt cgttatcaag 1381 tgtttaagca aagacttgag tgggacttag ttgtagaaaa taaccttgaa tcagatgagt 1441 atgataactc aaatgcagaa tatatttatg cttgtgatga tactgaaaat gtaagtggat 1501 gctggcgttt attacctaca acaggtgatt atatgctgaa aagtgttttt cctgaattgc 1561 ttggtcaaca gagtgctccc aaagatccta atatagtcga attaagtcgt tttgctgtag 1621 gtaaaaatag ctcaaagata aataactctg ctagtgaaat tacaatgaaa ctatttgaag 1681 ctatatataa acacgctgtt agtcaaggta ttacagaata tgtaacagta acatcaacag 1741 caatagagcg atttttaaag cgtattaaag ttccttgtca tcgtattgga gacaaagaaa 1801 ttcatgtatt aggtgatact aaatcggttg tattgtctat gcctattaat gaacagttta 1861 aaaaagcagt cttaaattaa taaattaaag gcatcaaata aaacgaaagg ctcagtcgaa 1921 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa 1981 tccgccgccc tagacagctg ggcgcgccaa atacgcccgg tagtgatctt atttcattat 2041 ggtgaaagtt ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc 2101 agggcttccc ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg 2161 tcacaggtat ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt 2221 gtatgatggt gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt 2281 tcagctactg acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg 2341 agtgtatact ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg 2401 caggagaaaa aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc 2461 ttcctcgctc actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta 2521 cgaacggggc ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg 2581 gccgcggcaa agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga 2641 cgctcaaatc agtggtggcg aaacccgaca ggactataaa gataccaggc gtttcccctg 2701 gcggctccct cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt 2761 tatggccgcg tttgtctcat tccacgcctg acactcagtt ccgggtaggc agttcgctcc 2821 aagctggact gtatgcacga accccccgtt cagtccgacc gctgcgcctt atccggtaac 2881 tatcgtcttg agtccaaccc ggaaagacat gcaaaagcac cactggcagc agccactggt 2941 aattgattta gaggagttag tcttgaagtc atgcgccggt taaggctaaa ctgaaaggac 3001 aagttttggt gactgcgctc ctccaagcca gttacctcgg ttcaaagagt tggtagctca 3061 gagaaccttc gaaaaaccgc cctgcaaggc ggttttttcg ttttcagagc aagagattac 3121 gcgcagacca aaacgatctc aagaagatca tcttattaat cagataaaat atttctagca 3181 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 3241 ttccccgaaa agtgccacct gctagggccg gcctacggcc agcctcgcag agcaggattc 3301 ccgttgagca ccgccaggtg cgaataaggg acagtgaaga aggaacaccc gctcgtgggt 3361 gggcctactt cacctatcct gcccggctga cgccgttgga tacaccaagg aaagtctaca 3421 cgaacccttt ggcaaaatcc tgtatatcgt gcgaaaaagg atggatatac cgaaaaaatc 3481 gctataatga ccccgaagca gggttatgca gcggaaaagg acccttgtcc aattaattat 3541 tagaaaaatt catccagcat cagatgaaat tgcagtttgt tcatatccgg attatcaatg 3601 ccatatttct gaaacagacg tttttgcagg ctcgggctaa attcgcccag gcagttccac 3661 agaatggcca gatcctgata acgatccgca atgcccacac ggcccacatc aatgcagcca 3721 atcagtttgc cttcatcgaa aatcaggtta tccaggctaa aatcgccgtg ggtcaccacg 3781 ctatccgggc taaacggcag cagtttatgc atttctttcc acacctgttc caccggccag 3841 ccgttacgtt catcatcaaa atcgctcgca tccaccaggc cgttgttcat acggctctgc 3901 gcctgggcca gacgaaacac acgatcgctg ttaaacgggc agttgcacac cggaatgcta 3961 tgcagacgac gcagaaacac ggccagcgca tccacaatgt tttcgccgct atccggatat 4021 tcttccagca cctgaaacgc ggttttgccc ggaatcgcgg tggtcagcag ccacgcatca 4081 tccggggtgc gaataaaatg tttaatggtc ggcagcggca taaattcggt cagccagttc 4141 agacgcacca tttcatcggt cacatcgttc gccacgctgc ctttgccatg tttcagaaac 4201 agttccggcg catccggttt gccatacaga cgataaatgg tcgcgccgct ctgacccacg 4261 ttatcacgcg cccatttata gccatacaga tccgcatcca tgttgctgtt cagacgcgga 4321 cggctacagc tcgtttcacg ctgaatatgg ctcataacac cccttgtatt actgtttatg 4381 taagcagaca gttttattgt tcatgatgat atatttttat cttgtgcaat gtaacatcag 4441 agattttgag acacaaattt //