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Template:Team:USyd-Australia/Calendar/Events List
From 2014.igem.org
(Difference between revisions)
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{ title: "Plasmid prep of psB6A1, Checking: finish digests, gel electrophoresis of digests", | { title: "Plasmid prep of psB6A1, Checking: finish digests, gel electrophoresis of digests", | ||
start: new Date(2014,6,10), | start: new Date(2014,6,10), | ||
| - | description: "Details: Gel electrophoresis (100V, 400mA, 30min)<br><br>Result: Gel broke. pSB6A1 yielded 5104.1ng/uL DNA & | + | description: "Details: Gel electrophoresis (100V, 400mA, 30min)<br><br>Result: Gel broke. pSB6A1 yielded 5104.1ng/uL DNA & (A260/280) 1.62.<br><br>Conclusion: Possible protein contamination to samples<br><br>- Abi"}, |
{ title: "Plasmid prep of pSB6A1, Re-doing digest", | { title: "Plasmid prep of pSB6A1, Re-doing digest", | ||
start: new Date(2014,6,11), | start: new Date(2014,6,11), | ||
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{ title: "Plasmid prep of pUS44, Re-doing digest", | { title: "Plasmid prep of pUS44, Re-doing digest", | ||
start: new Date(2014,6,17), | start: new Date(2014,6,17), | ||
| - | description: "Details: Restriction digest. Did the protocol change?<br><br>Conclusion: Nanospec: 395.5ng/mL DNA yield<br><br>- Abi & | + | description: "Details: Restriction digest. Did the protocol change?<br><br>Conclusion: Nanospec: 395.5ng/mL DNA yield<br><br>- Abi & Tom"}, |
{ title: "Construction of pUS201, PCR of pSB6A1 using iGEM401/402 primers ", | { title: "Construction of pUS201, PCR of pSB6A1 using iGEM401/402 primers ", | ||
start: new Date(2014,6,25), | start: new Date(2014,6,25), | ||
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{ title: "Construction of pUS204, Transformation: pUS204 into top10 cells", | { title: "Construction of pUS204, Transformation: pUS204 into top10 cells", | ||
start: new Date(2014,6,31), | start: new Date(2014,6,31), | ||
| - | description: "Details: Transformation (competent top10, pUS204). Incubated on AmpR plates for 2hrs<br><br>Result: Some AmpR colonies present, no blue colonies detected<br><br>Conclusion: pUS024 did not successfully transform, | + | description: "Details: Transformation (competent top10, pUS204). Incubated on AmpR plates for 2hrs<br><br>Result: Some AmpR colonies present, no blue colonies detected<br><br>Conclusion: pUS024 did not successfully transform, \"far unlikely positive control worked\" <br><br>- ?"}, |
{ title: "construction of pUS201, see notebook: second GA and transformation successful???", | { title: "construction of pUS201, see notebook: second GA and transformation successful???", | ||
start: new Date(2014,7,01), | start: new Date(2014,7,01), | ||
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{ title: "construction of pUS201, re-doing pSB1C3 PCR with iGEM1403/1404", | { title: "construction of pUS201, re-doing pSB1C3 PCR with iGEM1403/1404", | ||
start: new Date(2014,7,04), | start: new Date(2014,7,04), | ||
| - | description: "Details: PCR Master mix with 1403/1404 primers. Used iGEM psB1C3. Ran on regular program PCR ~2HRS<br><br>Result: Products stored in the fridge<br><br>- Jeanne & | + | description: "Details: PCR Master mix with 1403/1404 primers. Used iGEM psB1C3. Ran on regular program PCR ~2HRS<br><br>Result: Products stored in the fridge<br><br>- Jeanne & Callum"}, |
{ title: "construction of pus201, Gel purification: gel electrophoresis of pSB1C3 PCR ", | { title: "construction of pus201, Gel purification: gel electrophoresis of pSB1C3 PCR ", | ||
start: new Date(2014,7,05), | start: new Date(2014,7,05), | ||
| - | description: "Details: Gel purification electrophoresis (60V, 400mA, 1hr). Stained in gel red??? for 30min<br><br>Result: missing gel??<br><br>- Jeanne & | + | description: "Details: Gel purification electrophoresis (60V, 400mA, 1hr). Stained in gel red??? for 30min<br><br>Result: missing gel??<br><br>- Jeanne & Callum"}, |
{ title: "Checking pUS201 construct, Plasmid mini-Prep: to confirm pUS201 in top10 colonies", | { title: "Checking pUS201 construct, Plasmid mini-Prep: to confirm pUS201 in top10 colonies", | ||
start: new Date(2014,7,05), | start: new Date(2014,7,05), | ||
| - | description: "Details: Incubated overnight to finish later<br><br>Result: Yield 5.7ng/ul, 1.61 260/280<br><br>Conclusion: Yield too low to use<br><br>- Jeanne & | + | description: "Details: Incubated overnight to finish later<br><br>Result: Yield 5.7ng/ul, 1.61 260/280<br><br>Conclusion: Yield too low to use<br><br>- Jeanne & Callum"}, |
| - | { title: "Checking IntI1 from SamR | + | { title: "Checking IntI1 from SamR\'s construct, Restriction digest: SamR\'s pSB1C3+ARApBAD", |
start: new Date(2014,7,08), | start: new Date(2014,7,08), | ||
description: "Details: Restriction digest (EcoR1+Pst1), incubated at 37 C for 1hr<br><br>Result: Yield: 4.46 ug/uL. Stored in -20 C fridge<br><br>Conclusion: Yield much higher than estimate provided by SamR (50ng/uL). Useable<br><br>- Rokiah"}, | description: "Details: Restriction digest (EcoR1+Pst1), incubated at 37 C for 1hr<br><br>Result: Yield: 4.46 ug/uL. Stored in -20 C fridge<br><br>Conclusion: Yield much higher than estimate provided by SamR (50ng/uL). Useable<br><br>- Rokiah"}, | ||
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start: new Date(2014,7,12), | start: new Date(2014,7,12), | ||
description: "Details: Restriction Digest (uncut, EcoR1, HindIII, HindIII+BamHI)<br><br>Result: gel????? Results???<br><br>- ?"}, | description: "Details: Restriction Digest (uncut, EcoR1, HindIII, HindIII+BamHI)<br><br>Result: gel????? Results???<br><br>- ?"}, | ||
| - | { title: "Checking IntI1 from SamR | + | { title: "Checking IntI1 from SamR\'s construct, Checking: SamR\'s digests on gel electrohoresis", |
start: new Date(2014,7,12), | start: new Date(2014,7,12), | ||
description: "Details: Gel electrophoresis (Spe1, uncut) at 120V for 1hr. Stained in gel red for ~30min.<br><br>Result: [12.08 SamR gel]. Cut lanes travelled further than uncut, as expected.<br><br>Conclusion: NOTE:: notes at the end cannot be read<br><br>- Rokiah"}, | description: "Details: Gel electrophoresis (Spe1, uncut) at 120V for 1hr. Stained in gel red for ~30min.<br><br>Result: [12.08 SamR gel]. Cut lanes travelled further than uncut, as expected.<br><br>Conclusion: NOTE:: notes at the end cannot be read<br><br>- Rokiah"}, | ||
| - | { title: "Making IntI1 for pUS203, Restriction Digest: extracting SamR | + | { title: "Making IntI1 for pUS203, Restriction Digest: extracting SamR\'s AraC-pBAD from pSB1C3", |
start: new Date(2014,7,13), | start: new Date(2014,7,13), | ||
description: "Details: pSB1C3 with AraC-pBAD was incubated with *specific enzyme* at 80 C for 20 min. The enzyme was heat-killed<br><br>- andy"}, | description: "Details: pSB1C3 with AraC-pBAD was incubated with *specific enzyme* at 80 C for 20 min. The enzyme was heat-killed<br><br>- andy"}, | ||
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{ title: "Checkin pUS203, Patch plate Int1I-2 and SamR construct", | { title: "Checkin pUS203, Patch plate Int1I-2 and SamR construct", | ||
start: new Date(2014,8,11), | start: new Date(2014,8,11), | ||
| - | description: "Details: GA product cultures transferred to patch plates using 25x | + | description: "Details: GA product cultures transferred to patch plates using 25x \"small\" colonies and 25x\"large\" colonies.<br><br>Result: Colonies to be screened with junction PCR. Why patch plate?<br><br>Conclusion: ?<br><br>- ?"}, |
| - | { title: "Checking Pus203, Preperation of colony PCR of PUS203: of the | + | { title: "Checking Pus203, Preperation of colony PCR of PUS203: of the \"small\" and \"large\" colonies on patch plate", |
start: new Date(2014,8,12), | start: new Date(2014,8,12), | ||
description: "Details: PCR with Hs463a and Hs464 primers and Taq.<br><br>"}, | description: "Details: PCR with Hs463a and Hs464 primers and Taq.<br><br>"}, | ||
| - | { title: "Checking Pus203, colony PCR of PUS203: of the | + | { title: "Checking Pus203, colony PCR of PUS203: of the \"small\" and \"large\" colonies on patch plate", |
start: new Date(2014,8,15), | start: new Date(2014,8,15), | ||
description: "Details: PCR with Hs463a and Hs464 primers and Taq.<br><br>Result: Expected fragment size was 473bp. No data received. ????<br><br>Conclusion: ?? Re-do?<br><br>- rokiah"}, | description: "Details: PCR with Hs463a and Hs464 primers and Taq.<br><br>Result: Expected fragment size was 473bp. No data received. ????<br><br>Conclusion: ?? Re-do?<br><br>- rokiah"}, | ||
Revision as of 07:17, 14 October 2014
