|
|
| Line 63: |
Line 63: |
| | start: new Date(2014,3,8), | | start: new Date(2014,3,8), |
| | description: 'Details: PCR Master mix with 1403/1404 primers. Used iGEM psB1C3. Ran on regular program PCR ~2HRS<br><br>Result: Products stored in the fridge<br><br>- Jeanne & Callum'}, | | description: 'Details: PCR Master mix with 1403/1404 primers. Used iGEM psB1C3. Ran on regular program PCR ~2HRS<br><br>Result: Products stored in the fridge<br><br>- Jeanne & Callum'}, |
| - | { title: 'construction of pus201, Gel purification: gel electrophoresis of pSB1C3 PCR ',
| |
| - | start: new Date(2014,4,8),
| |
| - | description: 'Details: Gel purification electrophoresis (60V, 400mA, 1hr). Stained in gel red??? for 30min<br><br>Result: missing gel??<br><br>- Jeanne & Callum'},
| |
| - | { title: 'Checking IntI1 from SamR's construct, Checking: SamR's digests on gel electrohoresis',
| |
| - | start: new Date(2014,11,8),
| |
| - | description: 'Details: Gel electrophoresis (Spe1, uncut) at 120V for 1hr. Stained in gel red for ~30min.<br><br>Result: [12.08 SamR gel]. Cut lanes travelled further than uncut, as expected.<br><br>Conclusion: NOTE:: notes at the end cannot be read<br><br>- Rokiah'},
| |
| - | { title: 'Making IntI1 for pUS203, Restriction Digest: extracting SamR's AraC-pBAD from pSB1C3',
| |
| - | start: new Date(2014,12,8),
| |
| - | description: 'Details: pSB1C3 with AraC-pBAD was incubated with *specific enzyme* at 80 C for 20 min. The enzyme was heat-killed<br><br>- andy'},
| |
| - | { title: 'Construction of pUS204, Restrction digestion of aeBlue gBlock',
| |
| - | start: new Date(2014,13,8),
| |
| - | description: 'Details: Restriction Digestion (EcoR1+Pst1)<br><br>Result: Yield: 1.6ng/uL<br><br>Conclusion: Enough yield to continue with ligation<br><br>- abi and tom'},
| |
| - | { title: 'Checking pUS201 construct, Plasmid mini-Prep: to confirm pUS201 in top10 colonies',
| |
| - | start: new Date(2014,20,8),
| |
| - | description: 'Details: Plasmid prep<br><br>Result: Yield: 3680.1ng/uL. 2.03 A260/280<br><br>Conclusion: A260/280 high ratio, may have contamination<br><br>- Callum'},
| |
| - | { title: 'Construction of pUS204, Ligation of pSB1C3 and aeBlue gBlock digestion products',
| |
| - | start: new Date(2014,27,8),
| |
| - | description: 'Details: Ligation using T4 ligase and products. Incubated for 1 hr at room temperature and them left overnight in 4C. Enzymes heat-killed at 65C.<br><br>- Tom'},
| |
| - | { title: 'Construction of pUS204, Transformaiton of ligated products into Top10 cells',
| |
| - | start: new Date(2014,0,9),
| |
| - | description: 'Details: Transformation and then incubated overnight at 37 C<br><br>- Abi and Tom'},
| |
| - | { title: 'Checking pUS201 construct, Re-checking the pUS201 by restriction digestion',
| |
| - | start: new Date(2014,3,9),
| |
| - | description: 'Details: Restriction digestion (EcoR1, NdeI, NdeI/BamHI, NdeI/HindIII)<br><br>'},
| |
| - | { title: 'construction of PUS204, Junction PCR to check whether pUS204 has been ligated successfully',
| |
| - | start: new Date(2014,8,9),
| |
| - | description: 'Details: Junction PCR with iGEM1407/iGEM1408 primers and Taq<br><br>Result: Products plated for…why?<br><br>- Tom'},
| |
| - | { title: 'Construction of pUS203, Checking: heat-killing and running a gel of products',
| |
| - | start: new Date(2014,9,9),
| |
| - | description: 'Details: Heat killed construct at 80 C for 20 min. Ran a gel electrophoresis of products wfor 100V for 1hr.<br><br>Result: Gel failed.<br><br>Conclusion: Gibson Assembly attempted anyway, gel fail was not indication of no construct<br><br>- Tom'},
| |
| - | { title: 'Construction of pUS201, Plating out Pus201 Gibson Assembly',
| |
| - | start: new Date(2014,9,9),
| |
| - | description: 'Details: Plated out GA products onto Amp plates.<br><br>- Tom'},
| |
| - | { title: 'Construction of pUS204, Junction PCR gel',
| |
| - | start: new Date(2014,9,9),
| |
| - | description: 'Details: Gel electrophoresis with PCR products (overnight pSB1C3/aeBlue cultures, 1hr growth cultures, no plasmid colony, no template control)<br><br>Result: ??<br><br>Conclusion: ??<br><br>'},
| |
| - | { title: 'Checkin pUS203, Patch plate Int1I-2 and SamR construct',
| |
| - | start: new Date(2014,10,9),
| |
| - | description: 'Details: GA product cultures transferred to patch plates using 25x "small" colonies and 25x"large" colonies.<br><br>Result: Colonies to be screened with junction PCR. Why patch plate?<br><br>Conclusion: ?<br><br>- ?'},
| |
| - | { title: 'Checking Pus203, Preperation of colony PCR of PUS203: of the "small" and "large" colonies on patch plate',
| |
| - | start: new Date(2014,11,9),
| |
| - | description: 'Details: PCR with Hs463a and Hs464 primers and Taq.<br><br>'},
| |
| - | { title: 'Checking Pus203, colony PCR of PUS203: of the "small" and "large" colonies on patch plate',
| |
| - | start: new Date(2014,14,9),
| |
| - | description: 'Details: PCR with Hs463a and Hs464 primers and Taq.<br><br>Result: Expected fragment size was 473bp. No data received. ????<br><br>Conclusion: ?? Re-do?<br><br>- rokiah'},
| |
| | ] | | ] |
| | }); | | }); |
"
