Team:Yale

From 2014.igem.org

(Difference between revisions)
 
(58 intermediate revisions not shown)
Line 3: Line 3:
{{Template:Team:Yale2014/Templates/Header}}
{{Template:Team:Yale2014/Templates/Header}}
<html>
<html>
 +
<head>
 +
 +
</head>
<link href='http://fonts.googleapis.com/css?family=Montserrat:400,700' rel='stylesheet' type='text/css'>
<link href='http://fonts.googleapis.com/css?family=Montserrat:400,700' rel='stylesheet' type='text/css'>
<link href='http://fonts.googleapis.com/css?family=Open+Sans:400,700' rel='stylesheet' type='text/css'>
<link href='http://fonts.googleapis.com/css?family=Open+Sans:400,700' rel='stylesheet' type='text/css'>
<link href='http://fonts.googleapis.com/css?family=Roboto:400,700' rel='stylesheet' type='text/css'>
<link href='http://fonts.googleapis.com/css?family=Roboto:400,700' rel='stylesheet' type='text/css'>
-
 
-
 
<style>
<style>
#contentSub, #footer-box, #catlinks, #search-controls, #p-logo, .printfooter, .firstHeading,.visualClear {display: none;} /*-- hides default wiki settings --*/
#contentSub, #footer-box, #catlinks, #search-controls, #p-logo, .printfooter, .firstHeading,.visualClear {display: none;} /*-- hides default wiki settings --*/
-
*{
+
:not(.navmenubkg){
   font-family:'Open Sans';
   font-family:'Open Sans';
 +
}
 +
.navmenubkg {
 +
font-family:'Roboto';
 +
}
}
h1,h2,h3,h4,h5,h6{
h1,h2,h3,h4,h5,h6{
   font-family:'Roboto';
   font-family:'Roboto';
 +
}
 +
 +
#top-section{
 +
width:100%;
 +
margin-left:0;
 +
left:0;
 +
margin-bottom:5px;
 +
}
 +
 +
div#vidbutton {
 +
    position:absolute;
 +
    width:100px;
 +
    height:100px;
 +
    background-image:url('paper.gif');
 +
    left:720px;
 +
    top:550px;
}
}
</style>
</style>
<!--main content -->
<!--main content -->
-
<table width="70%" align="center" style="margin-top:44px">
+
<table width="1100px" align="center" style="margin-top:0px">
<!--welcome box -->
<!--welcome box -->
<tr>
<tr>
<td style="border:none;" colspan="3" align="center" height="150px">
<td style="border:none;" colspan="3" align="center" height="150px">
-
<h1 style="display:none">Biosynthesis of an Anti-biofouling Surface Binding Polymer with the 21st Amino Acid- L-Dopamine</h1>
+
<h1 style="display:none">Biosynthesis of an Anti-biofouling Surface Binding Polymer with the 21st Amino Acid- L-DOPA</h1>
<br>
<br>
-
<p> <img src="http://placehold.it/1100x328/004166/FFFFFFF/&text=Antimicrobial+Shielding" > </p>
+
<p> <img src="https://static.igem.org/mediawiki/2014/d/d7/Ampersand_for_Yale_iGEM-v2.png" width="1100"> <div id = "vidbutton"></div></p>
</td>
</td>
</tr>
</tr>
-
<tr> <td colspan="3"  height="5px"> </td></tr>
 
<!-- end welcome box -->
<!-- end welcome box -->
-
<tr>  
+
<tr><td colspan="3"><div class = "well" style="margin-bottom:10px"><p> <strong><center>Producing a Novel Antimicrobial Surface-Binding Peptide Using an Improved T7 Expression System</center></strong><p>
 +
Biofilm formation on surfaces is an issue in the medical field, naval industry, and other areas. We developed an anti-fouling peptide with two modular components: a mussel adhesion protein (MAP) anchor and LL-37, an antimicrobial peptide. MAPs can selectively attach to metal and organic surfaces via L-3,5-dihydroxyphenylalanine (L-DOPA), a nonstandard amino acid that was incorporated using a genetically recoded organism (GRO).  Because this peptide is toxic to the GRO in which it is produced, we designed a better controlled inducible system that limits basal expression. This was achieved through a novel T7 riboregulation system that controls expression at both the transcriptional and translational levels.  This improved system is a precise synthetic switch for the expression of cytotoxic substances in the already robust T7 system. Lastly, the antimicrobial surface-binding peptide was assayed for functionality.
 +
</p></div></td>
 +
</tr>
 +
<tr><div id = "show"><td colspan="3" style="padding-bottom:10px"><center><img src="https://static.igem.org/mediawiki/2014/5/56/Ampersand.png" width="150"></center></td></div></tr>
-
<tr> <td colspan="3" height="15px"> </td></tr>
+
<tr><td colspan="3"><a href="https://2014.igem.org/Team:Yale/Project"><img src="
-
<tr><td bgColor="#e7e7e7" colspan="3" height="1px"> </tr>
+
https://static.igem.org/mediawiki/2014/f/f8/Yale-Overview.png" width="1100"></a></td></tr>
-
<tr> <td colspan="3"  height="5px"> </td></tr>
+
 +
<tr><td colspan="3"><a href="https://2014.igem.org/Team:Yale/Team"><img src="https://static.igem.org/mediawiki/2014/5/50/Yale-Team.png"  width="1100"></a></td></tr>
-
<!--requirements section -->
+
<tr><td colspan="3"><a href="https://2014.igem.org/Team:Yale/Outreach"><img src="https://static.igem.org/mediawiki/2014/0/08/Yale_Outreach.pngwidth="1100"></a></td></tr>
-
<tr><td colspan="3"> <h3> Requirements </h3></td></tr>
+
-
<tr>
+
-
<td width="45%valign="top">  
+
-
<p> Please be sure to keep these links, your audience will want to find your: </p>
+
<tr><td colspan="3"><a href="https://2014.igem.org/Team:Yale/Interlab"><img src="https://static.igem.org/mediawiki/2014/3/3c/Yale_Interlab.png"  width="1100"></a></td></tr>
-
<!-- Links to other team pages -->
+
<tr><td colspan="3"><a href="https://2014.igem.org/Team:Yale/Notebook"><img src="https://static.igem.org/mediawiki/2014/2/2c/Yale_Notebook.png" width="1100"></a></td></tr>
-
<ul>
+
-
<li><a href="https://2014.igem.org/Team:Yale">Home</a> </li>
+
-
<li><a href="https://2014.igem.org/Team:Yale/Team">Team</a> </li>
+
-
<li><a href="https://igem.org/Team.cgi?year=2013&team_name=Yale">Official Team Profile</a> </li>
+
-
<li><a href="https://2014.igem.org/Team:Yale/Project">Project</a> </li>
+
-
<li><a href="https://2014.igem.org/Team:Yale/Parts">Parts</a> </li>
+
-
<li><a href="https://2014.igem.org/Team:Yale/Modeling">Modeling</a> </li>
+
-
<li><a href="https://2014.igem.org/Team:Yale/Notebook">Notebook</a> </li>
+
-
<li><a href="https://2014.igem.org/Team:Yale/Safety">Safety</a> </li>
+
-
<li><a href="https://2014.igem.org/Team:Yale/Attributions">Attributions</a> </li>
+
-
</ul>
+
<tr><td colspan="3"><a href="https://2014.igem.org/Team:Yale/Achievements"><img src="https://static.igem.org/mediawiki/2014/2/29/Yale_Achivements.png"  width="1100"></a></td></tr>
-
</td>
 
-
<td > </td>
 
-
<td width="45%">
 
-
<p>There are a few wiki requirements teams must follow:</p>
 
-
<ul>
 
-
<li>All pages, images and files must be hosted on the <a href ="https://2014.igem.org/Special:Upload">  2014.igem.org server</a>. </li>
 
-
<li>All pages must be created under the team’s name space.</li>
 
-
<li>As part of your documentation, keep the links from the menu to the left. </li>
 
-
<li>Do not use flash in wiki code. </li>
 
-
<li>The <a href="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png"> iGEM logo </a> should be placed on the upper part of every page and should link to <a href="https://2014.igem.org/Main_Page">2014.igem.org</a>.</li>
 
-
</ul>
 
-
<p>Visit the <a href="https://2014.igem.org/Wiki_How-To"> Wiki How To page </a> for a complete list of requirements, tips and other useful information. </p>
 
-
</td>
 
-
</tr>
 
-
<tr> <td colspan="3"  height="15px"> </td></tr>
 
-
<tr><td bgColor="#e7e7e7" colspan="3" height="1"> </tr>
 
-
<!--tips  -->
+
</table>
-
<tr><td colspan="3" > <h3> Tips  </h3></td></tr>
+
-
<tr>
+
<div width="1100px" align="center" >
-
<td width="45%" valign="top">
+
-
<p>We are currently working on providing teams with some easy to use design templates.
+
-
<br> In the meantime you can also view other team wikis for inspiration! Here are some very good examples</p>
+
-
<ul>
 
-
<li> <a href="https://2013.igem.org/Team:SDU-Denmark/"> 2013 SDU Denmark </a> </li>
 
-
<li> <a href="https://2013.igem.org/Team:SYSU-China">2013 SYSU China</a> </li>
 
-
<li> <a href="https://2013.igem.org/Team:Shenzhen_BGIC_ATCG"> 2013 Shenxhen BGIG ATCG </a></li>
 
-
<li> <a href="https://2013.igem.org/Team:Colombia_Uniandes">2013 Colombia Unianades </a></li>
 
-
<li> <a href="https://2013.igem.org/Team:Lethbridge">2013 Lethbridge</a></li>
 
-
</ul>
 
-
 
-
<p>For a full wiki list, you can visit <a href="https://igem.org/Team_Wikis?year=2013">iGEM 2013 web sites </a> and <a href="https://igem.org/Team_Wikis?year=2012">iGEM 2012 web sites</a>  lists. </p>
 
-
</td>
 
-
 
-
<td> </td>
 
-
<td width="45%">
 
-
 
-
<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
 
-
 
-
<ul>
 
-
<li>State your accomplishments! Tell people what you have achieved from the start. </li>
 
-
<li>Be clear about what you are doing and what you plan to do.</li>
 
-
<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
 
-
<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
 
-
<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
 
-
<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="">iGEM 2013 calendar</a> </li>
 
-
<li>Have lots of fun! </li>
 
-
</ul>
 
-
</br>
 
-
</td>
 
-
</tr>
 
-
</table>
 
 +
</div>
 +
</html>
{{Template:Team:Yale2014/Templates/Footer}}
{{Template:Team:Yale2014/Templates/Footer}}

Latest revision as of 03:37, 18 October 2014

Biosynthesis of an Anti-biofouling Surface Binding Polymer with the 21st Amino Acid- L-DOPA


Producing a Novel Antimicrobial Surface-Binding Peptide Using an Improved T7 Expression System

Biofilm formation on surfaces is an issue in the medical field, naval industry, and other areas. We developed an anti-fouling peptide with two modular components: a mussel adhesion protein (MAP) anchor and LL-37, an antimicrobial peptide. MAPs can selectively attach to metal and organic surfaces via L-3,5-dihydroxyphenylalanine (L-DOPA), a nonstandard amino acid that was incorporated using a genetically recoded organism (GRO). Because this peptide is toxic to the GRO in which it is produced, we designed a better controlled inducible system that limits basal expression. This was achieved through a novel T7 riboregulation system that controls expression at both the transcriptional and translational levels. This improved system is a precise synthetic switch for the expression of cytotoxic substances in the already robust T7 system. Lastly, the antimicrobial surface-binding peptide was assayed for functionality.

Main Campus:
Molecular, Cellular & Developmental Biology
219 Prospect Street
P.O. Box 208103
New Haven, CT 06520
Phone: 203.432.3783
igem@yale.edu
natalie.ma@yale.edu (Graduate Advisor)
Copyright (c) 2014 Yale IGEM