Team:Yale

From 2014.igem.org

(Difference between revisions)
Line 45: Line 45:
</p></div></td>
</p></div></td>
</tr>
</tr>
-
 
-
<tr><td colspan="3" align="center">
 
-
<iframe src="http://www.youtube.com/embed/H6iWGC0SrHE"
 
-
  width="600"  height="450"></iframe></td></tr>
 
-
 
<tr><td colspan="3"><a href="https://2014.igem.org/Special:Upload" target="_blank"><img src="http://placehold.it/1100x100/060343/FFFFFFF/&text=Upload+Files"></a></td></tr>
<tr><td colspan="3"><a href="https://2014.igem.org/Special:Upload" target="_blank"><img src="http://placehold.it/1100x100/060343/FFFFFFF/&text=Upload+Files"></a></td></tr>

Revision as of 02:43, 18 October 2014

Biosynthesis of an Anti-biofouling Surface Binding Polymer with the 21st Amino Acid- L-DOPA


Producing a Novel Antimicrobial Surface-Binding Peptide Using an Improved T7 Expression System

Biofilm formation on surfaces is an issue in the medical field, naval industry, and other areas. We developed an anti-fouling peptide with two modular components: a mussel adhesion protein (MAP) anchor and LL-37, an antimicrobial peptide. MAPs can selectively attach to metal and organic surfaces via L-3,5-dihydroxyphenylalanine (L-DOPA), a nonstandard amino acid that was incorporated using a genetically recoded organism (GRO). Because this peptide is toxic to the GRO in which it is produced, we designed a better controlled inducible system that limits basal expression. This was achieved through a novel T7 riboregulation system that controls expression at both the transcriptional and translational levels. This improved system is a precise synthetic switch for the expression of cytotoxic substances in the already robust T7 system. Lastly, the antimicrobial surface-binding peptide was assayed for functionality.

Main Campus:
Molecular, Cellular & Developmental Biology
219 Prospect Street
P.O. Box 208103
New Haven, CT 06520
Phone: 203.432.3783
igem@yale.edu
natalie.ma@yale.edu (Graduate Advisor)
Copyright (c) 2014 Yale IGEM