Team:XMU-China/Safety

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<img src="https://static.igem.org/mediawiki/2014/a/a6/Xmu_background_veir.jpg" width="100%" height="100%"/>
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</div>
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<!--welcome box -->
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<div id="warp_navbar_main">
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<tr>
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<td style="border:1px solid black;" colspan="3" align="center" height="150px" bgColor=#FF404B>
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<div id="nav_bar_frame" >
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<h1 >WELCOME TO iGEM 2014! </h1>
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<img class="navbar_frame" id="navbar_frame_left" src="https://static.igem.org/mediawiki/2014/c/cf/Xmu_navbar_left04.png" />
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<p>Your team has been approved and you are ready to start the iGEM season!
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<img class="navbar_frame" id="navbar_frame_right" src="https://static.igem.org/mediawiki/2014/8/88/Xmu_navbar_right02.png" />
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<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
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<br>
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:XMU-China/Safety&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
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<a href="#"><span class="nav_bar" id="nav_bar_01"> </span></a>
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<a href="#" class="nav_bar_full" id="nav_bar_full_01">Project</a>
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<tr> <td colspan="3" height="5px"> </td></tr>
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<ul class="navbar_sub">
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<img class="navbar_sub_x" id="navbar_sub_x_01" src="https://static.igem.org/mediawiki/2014/4/4e/Xmu_navbar_sub_01.png" />
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<tr>  
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<li><a href="https://2014.igem.org/Team:XMU-China/Project_Background">Background</a></li>
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<li><a href="https://2014.igem.org/Team:XMU-China/Project_ConicCurve">Conic Curve</a></li>
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<!--navigation menu -->
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<li><a href="https://2014.igem.org/Team:XMU-China/Project_Application">Application</a></li>
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<td align="center" colspan="3">
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<li><a href="https://2014.igem.org/Team:XMU-China/Project_PSystem">P System</a></li>
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<li><a href="https://2014.igem.org/Team:XMU-China/Project_Modelling">Modelling</a></li>
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<table  width="100%">
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<li><a href="https://2014.igem.org/Team:XMU-China/Project_FutureWork">Future Work</a></li>
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<tr heigth="15px"></tr>
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<li><a href="https://2014.igem.org/Team:XMU-China/Project_Interlab">Interlab</a></li>
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<tr heigth="75px">
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</ul>
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</li>
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<li id="navbar_main_02" class="navbar_main">
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<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
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<a href="#"><span class="nav_bar" id="nav_bar_02"> </span></a>
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<a href="https://2014.igem.org/Team:XMU-China"style="color:#000000">Home </a> </td>
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<a href="#" class="nav_bar_full" id="nav_bar_full_02" style="letter-spacing:-2px;">Notebook</a>
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<ul class="navbar_sub">
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<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>  
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<img class="navbar_sub_x" id="navbar_sub_x_02" src="https://static.igem.org/mediawiki/2014/e/e0/Xmu_navbar_sub_02.png" />
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<a href="https://2014.igem.org/Team:XMU-China/Team"style="color:#000000"> Team </a> </td>
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<li><a href="https://2014.igem.org/Team:XMU-China/Notebook_Protocol">Protocol</a></li>
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<li><a href="https://2014.igem.org/Team:XMU-China/Notebook_Labnotes">Lab notes</a></li>
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<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>  
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<li><a href="https://2014.igem.org/Team:XMU-China/Notebook_Calendar">Calendar</a></li>
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<a href="https://igem.org/Team.cgi?year=2014&team_name=XMU-China"style="color:#000000"> Official Team Profile </a></td>
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</ul>
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</li>
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<li id="navbar_main_03" class="navbar_main">
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<a href="https://2014.igem.org/Team:XMU-China/Project"style="color:#000000"> Project</a></td>
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<a href="#"><span class="nav_bar" id="nav_bar_03"> </span></a>
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<a href="#" class="nav_bar_full" id="nav_bar_full_03">Judging</a>
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<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>  
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<ul class="navbar_sub">
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<a href="https://2014.igem.org/Team:XMU-China/Parts"style="color:#000000"> Parts</a></td>
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<img class="navbar_sub_x" id="navbar_sub_x_03" src="https://static.igem.org/mediawiki/2014/7/7a/Xmu_navbar_sub_03.png" />
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<li><a href="https://2014.igem.org/Team:XMU-China/Judging_Parts">Parts</a></li>
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<li><a href="https://2014.igem.org/Team:XMU-China/Judging_Achievements">Achievements</a></li>
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<a href="https://2014.igem.org/Team:XMU-China/Modeling"style="color:#000000"> Modeling</a></td>
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</ul>
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</li>
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<li id="navbar_main_04" class="navbar_main">
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<a href="https://2014.igem.org/Team:XMU-China/Notebook"style="color:#000000"> Notebook</a></td>
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<a href="#"><span class="nav_bar" id="nav_bar_04"> </span></a>
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<a href="#" class="nav_bar_full" id="nav_bar_full_04">Team</a>
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<td style="border:1px solid black;" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>  
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<ul class="navbar_sub">
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<a href="https://2014.igem.org/Team:XMU-China/Safety"style=" color:#000000"> Safety </a></td>
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<img class="navbar_sub_x" id="navbar_sub_x_04" src="https://static.igem.org/mediawiki/2014/1/1f/Xmu_navbar_sub_04.png" />
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<li><a href="https://2014.igem.org/Team:XMU-China/Team_Members">Members</a></li>
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<td style="border:1px solid black;" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>  
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<li><a href="https://2014.igem.org/Team:XMU-China/Team_Attribution">Attribution</a></li>
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<a href="https://2014.igem.org/Team:XMU-China/Attributions"style="color:#000000"> Attributions </a></td>
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</ul>
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</li>
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<li id="navbar_main_05" class="navbar_main">
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<td align ="center"> <a href="https://2014.igem.org/Main_Page"> <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="55px"></a> </td>
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<a href="#"><span class="nav_bar" id="nav_bar_05"> </span></a>
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</tr>
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<a href="#" class="nav_bar_full" id="nav_bar_full_05">P&amp;P</a>
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<ul class="navbar_sub">
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<img class="navbar_sub_x" id="navbar_sub_x_05" src="https://static.igem.org/mediawiki/2014/d/de/Xmu_navbar_sub_05.png" />
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</tr>
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<li><a href="https://2014.igem.org/Team:XMU-China/PP_Overview">Overview</a></li>
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</tr>
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<li><a href="https://2014.igem.org/Team:XMU-China/PP_Newsletter">Newsletter</a></li>
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</td>
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<li><a href="https://2014.igem.org/Team:XMU-China/PP_Communication">Communication</a></li>
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<li><a href="https://2014.igem.org/Team:XMU-China/PP_Popularization">Popularization</a></li>
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<tr> <td colspan="3" height="15px"> </td></tr>
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<li><a href="https://2014.igem.org/Team:XMU-China/PP_Assistance">Assistance</a></li>
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<tr><td bgColor="#e7e7e7" colspan="3" height="1px"> </tr>
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<li id="navbar_sub_Acknowledgements"><a href="https://2014.igem.org/Team:XMU-China/PP_Acknowledgements">Acknowledgements</a></li>
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<tr> <td colspan="3" height="5px"> </td></tr>
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</ul>
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</li>
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<li id="navbar_main_06" class="navbar_main">
-
<!--safety content-->
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<a href="https://2014.igem.org/Team:XMU-China/Safety"><span class="nav_bar" id="nav_bar_06"> </span></a>
-
<tr><td > <h3> Welcome! </h3></td>
+
<a href="https://2014.igem.org/Team:XMU-China/Safety" class="nav_bar_full" id="nav_bar_full_06">Safety</a>
-
<td ></td >
+
</li>
-
<td > <h3> Timeline</h3></td>
+
</ul>
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</tr>
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</div>
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</div>
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<tr>
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</div>
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<td width="45%"  valign="top">  
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<!--Safety-->
-
<p> Visit the <a href="https://2014.igem.org/Safety" >Safety Hub</a> to see this year's safety requirements. The Safety Hub is the central page for everything related to safety in iGEM. You can also go there to learn about general biosafety topics, and how to think about the future implications of your project.</p>
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<div id="Safety">
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<div id="Safety_wd">
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<p style="text-align: center; font-size: 21px;">
-
 
+
    <span style="font-size: 29px;">SAFETY</span>
-
<br>
+
</p>
-
<h3> Edit this page!</h3>
+
<br/>
-
<p>
+
<p style="font-size: 16px;">
-
Please use this page to write about anything related to safety in your project. <!--Be sure to talk about both
+
    <span style="font-size: 20px;font-weight: 700;">General Laboratory Safety</span>
-
<ul>
+
</p>
-
<li> <a href=" ">Learn about lab Safety for Today</a></li>  
+
<hr width="100%" size="2">
-
<li> <a href="">Learn about Safety for the future of your project.</a></li>  
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<br/>
-
</ul>
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<p style="text-align: justify; text-indent: -24px; font-size: 16px; margin-left: 24px; font-weight: 700;">
-
-->
+
    <span style="font-family: Calibri;font-weight: 700;">-</span></span><span style="font-size: 16px;">What </span><span style="font-size: 16px;">measures </span><span style="font-size: 16px;">does school take</span><span style="font-size: 16px;"> to guarantee the safety </span><span style="font-size: 16px;">in</span><span style="font-size: 16px;"> laboratories?</span>
 +
</p>
 +
<p style="font-size: 16px;">
 +
    &nbsp;
 +
</p>
 +
<p style="text-align: justify; text-indent: -24px; font-size: 16px; margin-left: 24px; styleName: Normal;">
 +
    <span style="font-family: Calibri;">-</span><span style="font-family: Calibri;"> </span><span style="font-size: 16px;">School </span><span style="font-size: 16px;">has its own biosafety rules that are regulated by the Ministry of Environment Protection, P. R. China and our institution.</span><span style="font-size: 16px;"> And each</span><span style="font-size: 16px;"> </span><span style="font-size: 16px;">laboratory safety standards</span><span style="font-size: 16px;"> </span><span style="font-size: 16px;">are implemented strictly.</span>
</p>
</p>
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+
<p style="text-align: justify; text-indent: -24px; font-size: 16px; margin-left: 24px; styleName: Normal;">
-
<h3> Your Lab </h3>
+
    <span style="font-family: Calibri;">-</span><span style="font-family: Calibri;"> </span><span style="font-size: 16px;">Students are required to participate in the chemical experimental courses of safety and environmental protection. Students learn atrategies that can deal with unexpected incidents. Final exam is
-
 
+
-
 
+
-
<p> Use this section to tell us about your laboratory. Where is it located? What sort of equipment do you use every day? Have you decorated it for the summer? How do you look wearing a lab coat? Take pictures! Show off your space! </p>
+
-
<!--
+
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<gallery>
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Image:Example2_Lab_1.png|The building our lab is in!
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Image:Example2_Lab_2.png|The inside of our lab!
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Image:Example2_Lab_3.png|Team Member 3 doing an experiment
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Image:Example2_Lab_4.png|Working in biosafety cabinets
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Image:Example2_Lab_5.png|Team all gloved up and ready for work!
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Image:Example2_Lab_6.png|Equipment that we use to do SCIENCE!
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Image:Example2_Lab_7.png|We decorated this part of our lab
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Image:Example2_Lab_8.png|Whatever else you want
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</gallery>-->
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<td></td>
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<td width="45%"  valign="top">  
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<ul>
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<li> <b>Now :</b> Read the <a href="https://2014.igem.org/Safety">Safety Hub </a> and learn about safety in iGEM. Ask questions by emailing safety at <i> igem DOT org </i>. </li>
+
-
<li><b>Now - Jamboree:</b> Complete <b>Check-Ins</b> and receive approval before acquiring and using certain materials in your lab</li>
+
-
<li><b>Now - Wiki Freeze:</b> Edit this Safety page to tell us about what you're doing</li>
+
-
<li><b>June 9: </b>Submit the About Our Lab form.</li>
+
-
<li><b>Let us know by June 25 </b>if you will need an extension on the Preliminary Version, or your Preliminary Version will be significantly incomplete.</li>
+
-
<li><b>June 30: </b>Submit the Preliminary Version of the <b>Safety Form</b>.</li>
+
-
<li>Participate in Virtual Open Office Hours to ask questions and discuss safety topics (exact date to be determined).</li>
+
-
<li><b>September 1:</b> Submit the Final Version of the Safety Form.</li>
+
-
<li><b>October: </b> Wiki freeze (exact date to be determined)</li>
+
-
<li><b>October 30 - November 3: </b>GIANT JAMBOREE!</li>
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</ul>
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</td>
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</tr>
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<tr>
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 +
School requires each student to participate in the chemical experimental courses of safety and environmental protection. In the courses, teachers introduce students the matters needing attention and strategies&nbsp;in&nbsp;dealing&nbsp;with the unexpected incidents&nbsp;through various case. Eventually each have to take the course exam, </span><span style="font-size: 16px;">only to meet the requirements of students before they can enter the lab and experimentize.</span>
 +
</p>
 +
<p style="text-align: justify; text-indent: -24px; font-size: 16px; margin-left: 24px; styleName: Normal;">
 +
    <span style="font-family: Calibri;">-</span><span style="font-family: Calibri;"> </span><span style="font-size: 16px;">All laboratories are</span><span style="font-size: 16px;"> equipped with safety experimental equipment</span><span style="font-size: 16px;">s</span><span style="font-size: 16px;"> and relevant person in charge</span><span style="font-size: 16px;">.</span>
 +
</p>
 +
<p style="text-align: justify; text-indent: 32px; font-size: 16px; styleName: Normal;">
 +
    &nbsp;
 +
</p>
 +
<p style="text-align: justify; text-indent: 32px; font-size: 16px; styleName: Normal;">
 +
    &nbsp;
 +
</p>
 +
<p style="text-align: justify; text-indent: -24px; font-size: 16px; margin-left: 24px;font-weight: 700;">
 +
    <span style="font-family: Calibri;">-</span><span style="font-family: Calibri;"> </span><span style="font-size: 16px;">W</span><span style="font-size: 16px;">hat special measures</span><span style="font-size: 16px;"> do you take to</span><span style="font-size: 16px;"> </span><span style="font-size: 16px;">guarantee</span><span style="font-size: 16px;"> </span><span style="font-size: 16px;">the safety of </span><span style="font-size: 16px;">experiment</span><span style="font-size: 16px;">ers i</span><span style="font-size: 16px;">n view of the </span><span style="font-size: 16px;">experiments related to iGEM</span><span style="font-size: 16px;">?</span>
 +
</p>
 +
<p style="font-size: 16px;">
 +
    &nbsp;
 +
</p>
 +
<p style="text-align: justify; text-indent: -24px; font-size: 16px; margin-left: 24px; styleName: Normal;">
 +
    <span style="font-family: Calibri;">-</span><span style="font-family: Calibri;"> </span><span style="font-size: 16px;">Every member of the team</span><span style="font-size: 16px;"> </span><span style="font-size: 16px;">has to take the experimental test</span><span style="font-size: 16px;">, </span><span style="font-size: 16px;">which </span><span style="font-size: 16px;">iGEM tutors and postgraduate </span><span style="font-size: 16px;">assigned the</span><span style="font-size: 16px;"> topic. </span><span style="font-size: 16px;">Only the one who pass the exam </span><span style="font-size: 16px;">will be a</span><span style="font-size: 16px;">llowed to enter the laboratory and start</span><span style="font-size: 16px;"> experiment</span><span style="font-size: 16px;">s. And </span><span style="font-size: 16px;">there will be a graduate student </span><span style="font-size: 16px;">give </span><span style="font-size: 16px;">us</span><span style="font-size: 16px;"> a demonstration</span><span style="font-size: 16px;"> and help new players</span><span style="font-size: 16px;"> to correct the mistakes in </span><span style="font-size: 16px;">the experiment.</span>
 +
</p>
 +
<p style="text-align: justify; text-indent: -24px; font-size: 16px; margin-left: 24px; styleName: Normal;">
 +
    <span style="font-family: Calibri;">-</span><span style="font-family: Calibri;"> </span><span style="font-size: 16px;">E</span><span style="font-size: 16px;">ach </span><span style="font-size: 16px;">member</span><span style="font-size: 16px;"> shall formulate detailed experimental plan before the experiment, and </span><span style="font-size: 16px;">get </span><span style="font-size: 16px;">familiar with the experimental process, master the characteristics of reagent</span><span style="font-size: 16px;">s</span><span style="font-size: 16px;"> before the experiment. During the experiment, all the players need </span><span style="font-size: 16px;">take</span><span style="font-size: 16px;"> fully protective measures, including </span><span style="font-size: 16px;">wearing </span><span style="font-size: 16px;">experimental suits, goggles, gloves and masks.</span>
 +
</p>
 +
<p style="font-size: 16px;">
 +
    &nbsp;
 +
</p>
 +
<br/>
 +
<p style="font-size: 16px;">
 +
    <span style="font-size: 20px;font-weight: 700;">Project Safety</span>
 +
</p>
 +
<hr width="100%" size="2">
 +
<br/>
 +
<p style="text-align: justify; text-indent: -24px; font-size: 16px; margin-left: 24px; font-weight: 700;">
 +
    <span style="font-family: Calibri;">-</span><span style="font-family: Calibri;"> </span><span style="font-size: 16px;">Whether you use the </span><span style="font-size: 16px;">reagent</span><span style="font-size: 16px;">s with potential </span><span style="font-size: 16px;">hazard</span><span style="font-size: 16px;">?</span>
 +
</p>
 +
<p style="font-size: 16px;">
 +
    &nbsp;
 +
</p>
 +
<p style="text-align: justify; text-indent: -24px; font-size: 16px; margin-left: 24px; styleName: Normal;">
 +
    <span style="font-family: Calibri;">-</span><span style="font-family: Calibri;"> </span><span style="font-size: 16px;">Strain</span><span style="font-size: 16px;">s</span><span style="font-size: 16px;"> used in the experiments are </span><span style="font-size: 16px; font-style: italic;">E. coli</span><span style="font-size: 16px;"> </span><span style="font-size: 16px;">(</span><span style="font-size: 16px;">k)</span><span style="font-size: 16px;"> </span><span style="font-size: 16px;">which is</span><span style="font-size: 16px;"> non-pathogenic bacteria</span><span style="font-size: 16px;">.</span>
 +
</p>
 +
<p style="text-align: justify; text-indent: -24px; font-size: 16px; margin-left: 24px; styleName: Normal;">
 +
    <span style="font-family: Calibri;">-</span><span style="font-family: Calibri;"> </span><span style="font-size: 16px;">This year we improve the n</span><span style="font-size: 16px;">ucleic acid gel stain</span><span style="font-size: 16px;"> in the experiment, replacing the EB (e</span><span style="font-size: 16px;">thidium bromide</span><span style="font-size: 16px;">) with </span><span style="font-size: 16px;">SYBR Green I</span><span style="font-size: 16px;">. Although </span><span style="font-size: 16px;">SYBR Green I</span><span style="font-size: 16px;"> has potential </span><span style="font-size: 16px;">hazard</span><span style="font-size: 16px;">, the c</span><span style="font-size: 16px;">arcinogenicity </span><span style="font-size: 16px;">is </span><span style="font-size: 16px;">less than </span><span style="font-size: 16px;">EB.</span>
 +
</p>
 +
<p style="font-size: 16px;">
 +
    &nbsp;
 +
</p>
 +
<p style="font-size: 16px;">
 +
    &nbsp;
 +
</p>
 +
<p style="text-align: justify; text-indent: -24px; font-size: 16px; margin-left: 24px;font-weight: 700;">
 +
    <span style="font-family: Calibri;">-</span><span style="font-family: Calibri;"> </span><span style="font-size: 16px;">Did </span><span style="font-size: 16px;">the experiments</span><span style="font-size: 16px;"> pollute the environment?</span>
 +
</p>
 +
<p style="text-align: justify; text-indent: -24px; font-size: 16px; margin-left: 24px; styleName: Normal;">
 +
    <span style="font-family: Calibri;">-</span><span style="font-family: Calibri;"> </span><span style="font-size: 16px;">To avoid this potential biological pollution to environment, we gathered all culture fluid and culture medium with bacteria and we processed them together to ensure </span><span style="font-size: 16px;">that none of them leaked from our laboratory through sewer and dustbin. </span>
 +
</p>
 +
<p style="text-align: justify; text-indent: -24px; font-size: 16px; margin-left: 24px; styleName: Normal;">
 +
    <span style="font-family: Calibri;">-</span><span style="font-family: Calibri;"> </span><span style="font-size: 16px;">We handled all the biological materials, wastes and equipment strictly following the BSL-1 requirements. All these things that have physically contacted with biological samples were bleached by autoclave before being discarded or reused.</span>
 +
</p>
 +
<p style="text-align: justify; text-indent: 0em; font-size: 16px; margin-left: 24px; styleName: Normal;">
 +
    &nbsp;
 +
</p>
 +
<p style="text-align: justify; text-indent: -24px; font-size: 16px; margin-left: 24px;font-weight: 700;">
 +
    <span style="font-family: Calibri;">-</span><span style="font-family: Calibri;"> </span><span style="font-size: 16px;">What measures do you take to </span><span style="font-size: 16px;">guarantee</span><span style="font-size: 16px;"> the strains </span><span style="font-size: 16px;">won’t leak in the project</span><span style="font-size: 16px;">?</span>
 +
</p>
 +
<p style="font-size: 16px;">
 +
    &nbsp;
 +
</p>
 +
<p style="text-align: justify; text-indent: -24px; font-size: 16px; margin-left: 24px; styleName: Normal;">
 +
    <span style="font-family: Calibri;">-</span><span style="font-family: Calibri;"> </span><span style="font-size: 16px;">We designed a </span><span style="font-size: 16px;">black hole system</span><span style="font-size: 16px;"> which can kill the</span><span style="font-size: 16px;"> bacteria</span><span style="font-size: 16px;"> with </span><span style="font-size: 16px;">hyperosmotic pressure</span><span style="font-size: 16px;">. In the gene circuit, the higher </span><span style="font-size: 16px;">osmolality</span><span style="font-size: 16px;"> </span><span style="font-size: 16px;">result</span><span style="font-size: 16px;">s</span><span style="font-size: 16px;"> in stronger expression strength of Pomp</span><span style="font-size: 16px;">. Then t</span><span style="font-size: 16px;">he moving tendency of reprogrammed CL-1 will orient to the hyperosmotic pressure spot.</span><span style="font-size: 14px;"> </span><span style="font-size: 16px;">When the bacteria move into where the osmotic pressur</span><span style="font-size: 16px;">e reaches to the critical value, </span><span style="font-size: 16px;">the bacteria </span><span style="font-size: 16px;">will </span><span style="font-size: 16px;">suicide finally.</span><span style="font-size: 16px;"> </span><span style="font-size: 16px;">The sources to create hyperosmotic pressure are cheap, accessible, environmentally friendly.</span>
 +
</p>
 +
<p style="text-align: justify; text-indent: 0em; font-size: 16px; margin-left: 24px; styleName: Normal;">
 +
    &nbsp;
 +
</p>
 +
<p style="text-align: justify; text-indent: 0em; font-size: 16px; margin-left: 24px; styleName: Normal;">
 +
    &nbsp;
 +
</p>
 +
<br/>
 +
<p style="font-size: 16px;">
 +
    <span style="font-size: 20px;font-weight: 700;">Supplementary</span>
 +
</p>
 +
<hr width="100%" size="2">
 +
<br/>
 +
<p style="font-size: 16px;">
 +
    <span style="font-size: 16px;">When a freshman comes to our College of Chemisty and Chemical E</span><span style="font-size: 16px;">ngineering in Xiamen University</span><span style="font-size: 16px;">,</span><span style="font-size: 16px;"> </span><span style="font-size: 16px;">, there i</span><span style="font-size: 16px;">s a story about &quot;C3H3&quot; for them</span><span style="font-size: 16px;">,</span><span style="font-size: 16px;"> </span><span style="font-size: 16px;">which was advocated by Prof. Jiaxi Lu, a famous Chinese quantum chemist. &quot;C3H3&quot; consists of three phrases, Clear Head, Clever Hand and Clean Habit. We think it is very useful when we do synthetic biological experiments, let alone talking about safety in genetic engineering.</span>
 +
</p>
 +
<p style="font-size: 16px;">
 +
    &nbsp;
 +
</p>
 +
<p style="font-size: 16px;">
 +
    <span style="font-size: 16px;">Firstly, before doing a genetic engineering experiment, you should keep your head clear. Concretely speaking, you need to find out the safety level of biological materials used in your experiment, including microorganisms, reagents and your constructed gene circuits. Keep every tiny experimental detail in your mind. This can help you avoid many potential safety hazards in your experiment.</span>
 +
</p>
 +
<p style="font-size: 16px;">
 +
    &nbsp;
 +
</p>
 +
<p style="font-size: 16px;">
 +
    <span style="font-size: 16px;">In addition, to make your hands clever means to perform a experiment skillfully. For example, when making a gel for SDS-PAGE, it is necessary to use acrylamide, which has strong carcinogenicity. So you have to pay much attention when mixing it and pouring the mixture into a mold to agglomerate.</span>
 +
</p>
 +
<p style="font-size: 16px;">
 +
    &nbsp;
 +
</p>
 +
<p style="font-size: 16px;">
 +
    <span style="font-size: 16px;">Last but not least, a clean habit is totally useful not only in your lab but also in your life. Imaging that, a mass, dirty and disorder lab, maybe some tattered gloves or broken, flasks, which have been contacted with highly toxic reagents are hidden below the experiment rig. Many risks would arise if there&#39;s unclean experimental habit. A clean habit is more than a combination of clear head and clever hands. It&#39;s a kind of decorous attitude to your experiments and a neat working atmosphere you create by yourself. A clean and good habit can improve your whole life.</span>
 +
</p>
 +
<p style="font-size: 16px;">
 +
    &nbsp;
 +
</p>
 +
<p style="font-size: 16px;">
 +
    <span style="font-size: 16px;">So this is our &quot;C3H3&quot;. We sincerely hope this proposal can improve safety in synthetic </span><span style="font-size: 16px;">biology and genetic engineering.</span>
 +
</p>
 +
<p style="font-size: 16px;">
 +
    &nbsp;
 +
</p>
 +
<p style="font-size: 16px;">
 +
    <span style="font-size: 16px;">Furthermore, we have another reasonable suggestion that every iGEM team should make a data table or survey results about public biosafety or laboratory safety and security of the projects. Every team can submit a survey report attached with questionnaire and analyzing data to the iGEM safety committee. More details are to be discussed.</span>
 +
</p>
 +
<p style="font-size: 30px;">
 +
    &nbsp;
 +
</p>
 +
</div>
 +
</div>
 +
 +
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{{:Team:XMU-China/footer}}

Latest revision as of 03:43, 18 October 2014

side_bar

SAFETY


General Laboratory Safety


-What measures does school take to guarantee the safety in laboratories?

 

- School has its own biosafety rules that are regulated by the Ministry of Environment Protection, P. R. China and our institution. And each laboratory safety standards are implemented strictly.

- Students are required to participate in the chemical experimental courses of safety and environmental protection. Students learn atrategies that can deal with unexpected incidents. Final exam is School requires each student to participate in the chemical experimental courses of safety and environmental protection. In the courses, teachers introduce students the matters needing attention and strategies in dealing with the unexpected incidents through various case. Eventually each have to take the course exam, only to meet the requirements of students before they can enter the lab and experimentize.

- All laboratories are equipped with safety experimental equipments and relevant person in charge.

 

 

- What special measures do you take to guarantee the safety of experimenters in view of the experiments related to iGEM?

 

- Every member of the team has to take the experimental test, which iGEM tutors and postgraduate assigned the topic. Only the one who pass the exam will be allowed to enter the laboratory and start experiments. And there will be a graduate student give us a demonstration and help new players to correct the mistakes in the experiment.

- Each member shall formulate detailed experimental plan before the experiment, and get familiar with the experimental process, master the characteristics of reagents before the experiment. During the experiment, all the players need take fully protective measures, including wearing experimental suits, goggles, gloves and masks.

 


Project Safety


- Whether you use the reagents with potential hazard?

 

- Strains used in the experiments are E. coli (k) which is non-pathogenic bacteria.

- This year we improve the nucleic acid gel stain in the experiment, replacing the EB (ethidium bromide) with SYBR Green I. Although SYBR Green I has potential hazard, the carcinogenicity is less than EB.

 

 

- Did the experiments pollute the environment?

- To avoid this potential biological pollution to environment, we gathered all culture fluid and culture medium with bacteria and we processed them together to ensure that none of them leaked from our laboratory through sewer and dustbin.

- We handled all the biological materials, wastes and equipment strictly following the BSL-1 requirements. All these things that have physically contacted with biological samples were bleached by autoclave before being discarded or reused.

 

- What measures do you take to guarantee the strains won’t leak in the project?

 

- We designed a black hole system which can kill the bacteria with hyperosmotic pressure. In the gene circuit, the higher osmolality results in stronger expression strength of Pomp. Then the moving tendency of reprogrammed CL-1 will orient to the hyperosmotic pressure spot. When the bacteria move into where the osmotic pressure reaches to the critical value, the bacteria will suicide finally. The sources to create hyperosmotic pressure are cheap, accessible, environmentally friendly.

 

 


Supplementary


When a freshman comes to our College of Chemisty and Chemical Engineering in Xiamen University, , there is a story about "C3H3" for them, which was advocated by Prof. Jiaxi Lu, a famous Chinese quantum chemist. "C3H3" consists of three phrases, Clear Head, Clever Hand and Clean Habit. We think it is very useful when we do synthetic biological experiments, let alone talking about safety in genetic engineering.

 

Firstly, before doing a genetic engineering experiment, you should keep your head clear. Concretely speaking, you need to find out the safety level of biological materials used in your experiment, including microorganisms, reagents and your constructed gene circuits. Keep every tiny experimental detail in your mind. This can help you avoid many potential safety hazards in your experiment.

 

In addition, to make your hands clever means to perform a experiment skillfully. For example, when making a gel for SDS-PAGE, it is necessary to use acrylamide, which has strong carcinogenicity. So you have to pay much attention when mixing it and pouring the mixture into a mold to agglomerate.

 

Last but not least, a clean habit is totally useful not only in your lab but also in your life. Imaging that, a mass, dirty and disorder lab, maybe some tattered gloves or broken, flasks, which have been contacted with highly toxic reagents are hidden below the experiment rig. Many risks would arise if there's unclean experimental habit. A clean habit is more than a combination of clear head and clever hands. It's a kind of decorous attitude to your experiments and a neat working atmosphere you create by yourself. A clean and good habit can improve your whole life.

 

So this is our "C3H3". We sincerely hope this proposal can improve safety in synthetic biology and genetic engineering.

 

Furthermore, we have another reasonable suggestion that every iGEM team should make a data table or survey results about public biosafety or laboratory safety and security of the projects. Every team can submit a survey report attached with questionnaire and analyzing data to the iGEM safety committee. More details are to be discussed.

 

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