Week Ten- August 4-10

Presented our preliminary results to Pakrasi Group and all of the advisors.
Ben started and tested new positive control and found that it did not fluoresce as expected and suspect a faulty RBS. Started design of primers to modify the RBS of the system.

School year started.
Rebstock group continued to use acetylene reduction assay to test nitrogen fixation activity of JM109 and WM1788 under known optimal conditions.
Meanwhile Rebstock group started to plan specific protocol and design for BioBricking the nif plasmid.
Ben starting cloning project to test whether switching the rbs to a comparable one would fix the EYFP expression in the light plasmids. He sent the clones in for sequencing. Ben also worked on the title and abstract for the iGEM September 1st deadline.