Week Ten- August 4-10

Presented our preliminary results to Pakrasi Group and all of the advisors.
Ben started and tested new positive control and found that it did not fluoresce as expected and suspect a faulty RBS. Started design of primers to modify the RBS of the system.

School year started.
Continued cloning projects and troubleshooting.
Ben starting cloning for modified RBS positive control to test disfunctional reporter protein.
Started biobricking.
Caroline continued work with the positive control and started biobricking the nif cluster.
Richard continued Acetylene Reduction Assays
Ben confirmed that Ptet was the issue and swapped out tet promoters to a functional one. Cheryl helped Ben to swap out reporter genes into the light plasmids, and Ben successfully sent in two parts for biobricking.
Website started mass compiling information and formatting. Discovered Kompozer to format all the text. Worked on protocol page and notebook pages, waiting for data and graphs to fill pages.