Team:Warwick/Interlab

From 2014.igem.org

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'''WAQ'S WORK'''
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<h1> The Interlab Study </h1> <br> <br>
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We are taking part in the interlab study, where we will obtain and measure fluorescence data from three specific genetic devices expressing Green fluorescent protein (GFP) in E. coli . To view how we are going to do this experimentally and obtained results, click on the buttons below.
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<h2> Introduction </h2>
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DAY ONE
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<p> I read the interlab study as an attempt by iGEM HQ to conduct a comparative analysis of the different methods employed by the varied and diverse teams internationally to arrive at useful data. A key problem in science is ascertaining absolute measurements; there is no point in one measuring the fluorescence of some given part, only to arrive at arbitrary units whose value for other scientists is near zero. Standards must be set, in order to embue our results with any useful meaning. Although its motivations are shrouded in mystery, I percieve the interlab study as the first step towards that ultimate goal of blanket standardisation in a synthetic biological context. </p> <br> <br>
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Transformation of E. coli with devices following protocols found here (link to Notebook section)
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<p> It is suggested that a team conduct the interlab study as a preamble to the main event. However, we are the first example of an iGEM team at Warwick, and coalesced rather late in the day. Hence we decided just to dedicate two members of the team to pursuing it in parallel to our other endeavours, as a sort of side quest. </p> <br> <br>
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DAY TWO
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<h2> The Brief </h2>
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Transformations worked, going to regrow culture and then digest, ligate!
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<p> The brief was thus: construct three given devices in plasmid format, all of which express green fluorescent protein (GFP), transfect them into E.coli and finally measure the fluorescence of these bacteria, using whatever technique you deem reasonable.
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'''DAN'S WORK'''
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The Interlab study is a voluntary project, new this year, which exists separate to each team's core work, and is an opportunity for iGEM HQ to understand how much variability exists between the practices and measurement methodologies of teams from across the globe. We decided to participate not only to support this objective, but also out of interest and as a means of diversification; the greater the number of strands along which we work, the less damaging any particular failure would be. It is suggested as a pre-summer activity but we only began in late July so it necessarily ran parallel with our principal work.
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The brief was thus: construct three given devices in plasmid format, all of which express green fluorescent protein (GFP), transfect them into E.coli and finally measure the fluorescence of these bacteria, using whatever technique you deem reasonable.
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Revision as of 13:24, 28 August 2014

The Interlab Study



Introduction

I read the interlab study as an attempt by iGEM HQ to conduct a comparative analysis of the different methods employed by the varied and diverse teams internationally to arrive at useful data. A key problem in science is ascertaining absolute measurements; there is no point in one measuring the fluorescence of some given part, only to arrive at arbitrary units whose value for other scientists is near zero. Standards must be set, in order to embue our results with any useful meaning. Although its motivations are shrouded in mystery, I percieve the interlab study as the first step towards that ultimate goal of blanket standardisation in a synthetic biological context.



It is suggested that a team conduct the interlab study as a preamble to the main event. However, we are the first example of an iGEM team at Warwick, and coalesced rather late in the day. Hence we decided just to dedicate two members of the team to pursuing it in parallel to our other endeavours, as a sort of side quest.



The Brief

The brief was thus: construct three given devices in plasmid format, all of which express green fluorescent protein (GFP), transfect them into E.coli and finally measure the fluorescence of these bacteria, using whatever technique you deem reasonable.

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