Team:Warwick/Interlab

From 2014.igem.org

(Difference between revisions)
Line 12: Line 12:
'''DAN'S WORK'''
'''DAN'S WORK'''
 +
 +
The Interlab study is a voluntary project, new this year, which exists separate to each team's core work, and is an opportunity for iGEM HQ to understand how much variability exists between the practices and measurement methodologies of teams from across the globe. We decided to participate not only to support this objective, but also out of interest and as a means of diversification; the greater the number of strands along which we work, the less damaging any particular failure would be. It is suggested as a pre-summer activity but we only began in late July so it necessarily ran parallel with our principal work.
 +
 +
The brief was thus: construct and measure three specific devices, all available in full or part form in the kit plates distributed this year

Revision as of 14:05, 20 August 2014

WAQ'S WORK

We are taking part in the interlab study, where we will obtain and measure fluorescence data from three specific genetic devices expressing Green fluorescent protein (GFP) in E. coli . To view how we are going to do this experimentally and obtained results, click on the buttons below.

DAY ONE

Transformation of E. coli with devices following protocols found here (link to Notebook section)

DAY TWO

Transformations worked, going to regrow culture and then digest, ligate!

DAN'S WORK

The Interlab study is a voluntary project, new this year, which exists separate to each team's core work, and is an opportunity for iGEM HQ to understand how much variability exists between the practices and measurement methodologies of teams from across the globe. We decided to participate not only to support this objective, but also out of interest and as a means of diversification; the greater the number of strands along which we work, the less damaging any particular failure would be. It is suggested as a pre-summer activity but we only began in late July so it necessarily ran parallel with our principal work.

The brief was thus: construct and measure three specific devices, all available in full or part form in the kit plates distributed this year