Team:Warwick/Interlab
From 2014.igem.org
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<li style="list-style-type: none;"><a href="#Introduction"><span class="tocnumber"> 1. </span><span class="toctext"> Introduction </span></a></li> | <li style="list-style-type: none;"><a href="#Introduction"><span class="tocnumber"> 1. </span><span class="toctext"> Introduction </span></a></li> | ||
- | <li style="list-style-type: none;"><a href="#Timeline"><span class="tocnumber">2. </span><span class="toctext"> The experimental timeline </span></a></li> | + | <li style="list-style-type: none;"><a href="#Timeline"><span class="tocnumber"> 2. </span><span class="toctext"> The experimental timeline </span></a></li> |
- | <li style="list-style-type: none;"><a href="#Protocols"><span class="tocnumber">3. </span><span class="toctext"> Protocols and methodology </span></a></li> | + | <li style="list-style-type: none;"><a href="#Protocols"><span class="tocnumber"> 3. </span><span class="toctext"> Protocols and methodology </span></a></li> |
- | <li style="list-style-type: none;><a href="#Measurements"><span class="tocnumber">4. </span><span class="toctext"> Measurements and results </span></a></li> | + | <li style="list-style-type: none;><a href="#Measurements"><span class="tocnumber"> 4. </span><span class="toctext"> Measurements and results </span></a></li> |
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- | < | + | <h1 id="Introduction"> Introduction </h1> <br> |
- | <p> It is suggested that a team conduct the interlab study as a preamble to the main event. However, we are the first example of an iGEM team at Warwick, and coalesced rather late in the day. Hence we decided just to dedicate | + | <p> |
+ | The interlab study is an attempt by iGEM HQ to conduct a comparative analysis of the different methods employed by the varied and diverse teams internationally to arrive at useful data. A key problem in science is ascertaining absolute measurements; there is no point in one measuring the fluorescence of some given part, only to arrive at arbitrary units whose meaning to other scientists is near zero. Standards must be set, in order to embue our results with any useful meaning. The interlab study is a step towards that ultimate goal of blanket standardisation in the synthetic biological context. | ||
+ | </p> | ||
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+ | <p> | ||
+ | It is suggested that a team conduct the interlab study as a preamble to the main event. However, we are the first example of an iGEM team at Warwick, and coalesced rather late in the day. Hence we decided just to dedicate three members of the team to pursuing it in parallel to our other endeavours, as a side quest. It was primarily undertaken by <b> Dan Goss </b>, <b> Waqar Yousaf </b> and <b> Chelsey Tye </b>, with support from advisers <b> Will Rostain </b> and <b> Sian Davies </b>. | ||
+ | </p> | ||
<h1 id="Timeline"> The experimental timeline </h1> | <h1 id="Timeline"> The experimental timeline </h1> | ||
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<h1 id="Protocols"> Protocols and methodology </h1> | <h1 id="Protocols"> Protocols and methodology </h1> | ||
- | < | + | <p> Many of the protocols mentioned above which we used were harvested straight from the iGEM website, but we also used content from previous iGEM teams and instructions packaged with kits. The specific materials and procedures can be accessed through clicking the relevant hyperlink </p> |
<li> <a href="http://parts.igem.org/Help:Protocols/Transformation">Transformation </a> </li> | <li> <a href="http://parts.igem.org/Help:Protocols/Transformation">Transformation </a> </li> | ||
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- | <h1 id="Measurements"> Measurements and results </ | + | <h1 id="Measurements"> Measurements and results </h1> |
Revision as of 10:01, 16 October 2014
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Introduction
The interlab study is an attempt by iGEM HQ to conduct a comparative analysis of the different methods employed by the varied and diverse teams internationally to arrive at useful data. A key problem in science is ascertaining absolute measurements; there is no point in one measuring the fluorescence of some given part, only to arrive at arbitrary units whose meaning to other scientists is near zero. Standards must be set, in order to embue our results with any useful meaning. The interlab study is a step towards that ultimate goal of blanket standardisation in the synthetic biological context.
It is suggested that a team conduct the interlab study as a preamble to the main event. However, we are the first example of an iGEM team at Warwick, and coalesced rather late in the day. Hence we decided just to dedicate three members of the team to pursuing it in parallel to our other endeavours, as a side quest. It was primarily undertaken by Dan Goss , Waqar Yousaf and Chelsey Tye , with support from advisers Will Rostain and Sian Davies .
The experimental timeline
Timeline of when protocols were run and measurements were taken is listed belowThe remit of the interlab study boils down to constructing and characterising, albeit minimally, three devices. They share a lot of similarities, and the objective is obviously not to create some wacky new form of life, but to measure well characterised and well understood parts in order to measure the measuring equipment, as it were. I will quickly describe the nature of these devices.
Protocols and methodology
Many of the protocols mentioned above which we used were harvested straight from the iGEM website, but we also used content from previous iGEM teams and instructions packaged with kits. The specific materials and procedures can be accessed through clicking the relevant hyperlink