Team:Vanderbilt/Project

From 2014.igem.org

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bullet list of key points to mention in full description
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bullet list of key points to mention in full description:
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General:
- opening about how in plants as in no other kingdom of life, evolutionary pressure has directed the sculpting of incredibly meticulous pathways for the synthesis of organic compounds
- opening about how in plants as in no other kingdom of life, evolutionary pressure has directed the sculpting of incredibly meticulous pathways for the synthesis of organic compounds
- the exactly precise machinery for organosynthesis found among plants provide an exceptional platform for biological engineering
- the exactly precise machinery for organosynthesis found among plants provide an exceptional platform for biological engineering
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- ability to synthesize wide range of complex molecules with enormous human importance, from fragrances and flavorings to pesticides and pharmaceuticals
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- ability to synthesize wide range of complex molecules with enormous human importance, from fragrances and flavorings to pesticides and pharmaceuticals (see Verporte 1999)
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- idea of the project is to start along the path toward harnessing biotechnology as a novel means to produce these previous compounds on an industrial scale
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- project could have future applications in transforming the food industry (better nutrition, better tasting alternatives to fattening/unhealthy flavorings, improve quality generally), better the environment(use of safe and natural insecticides and fungicides derived from plant extracts, protect at risk plant species from harvesting), and health (range of drug candidates currently being explored for everything from cancer treatment to improved aseptics against infections)
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Methods:
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- exploit GPP/FPP pathway found naturally in yeast as starting material. Improves efficiency by eliminating rate-limiting steps and offers greater flexibility in manipulation to improve yields  (Farhi 2011)
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- go over all advantages of plasmid vector that was designed- bacterial and eukaryotic promotors, floxed elements, linearizable for homologous recombination, multiple resistance markers, MCS, etc.
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- innovation over previous years' teams of integrating into yeast genome and making diploid to improve yield of product
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- note how the project was designed, with large groups working in the lab and staged/tiered work structure is moving closer to a mass-production approach
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Revision as of 01:54, 29 July 2014



WELCOME TO iGEM 2014!

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Project Description

Content

Tell us more about your project. Give us background. Use this as the abstract of your project. Be descriptive but concise (1-2 paragraphs)


References

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You can use these subtopics to further explain your project

  1. Overall project summary
  2. Project Details
  3. Materials and Methods
  4. The Experiments
  5. Results
  6. Data analysis
  7. Conclusions

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