Team:Vanderbilt/Project
From 2014.igem.org
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<a href="https://2014.igem.org/Team:Vanderbilt/Attributions"style="color:#CC9900"> Attributions </a><br> | <a href="https://2014.igem.org/Team:Vanderbilt/Attributions"style="color:#CC9900"> Attributions </a><br> | ||
<img src="https://static.igem.org/mediawiki/parts/0/0e/Attributions_page_clipart.jpg" width="180px"></td> | <img src="https://static.igem.org/mediawiki/parts/0/0e/Attributions_page_clipart.jpg" width="180px"></td> | ||
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<td align ="center"> <a href="https://2014.igem.org/Main_Page"> <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="55px"></a> </td> | <td align ="center"> <a href="https://2014.igem.org/Main_Page"> <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="55px"></a> </td> | ||
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Main essential oil: Cade oil<br> | Main essential oil: Cade oil<br> | ||
Applications: Antifungal, bactericidal, and antioxidant<br> | Applications: Antifungal, bactericidal, and antioxidant<br> | ||
- | + | Plant Species: <i>Gossypium hirsutum</i> (cotton) | |
+ | Synthase Gene: δ-cadenine synthase (E.C. 4.2.3.13) | ||
</td> | </td> | ||
<td align="center" valign="center"> | <td align="center" valign="center"> | ||
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Main essential oils: Rosemary and Cedar oil<br> | Main essential oils: Rosemary and Cedar oil<br> | ||
Applications: Insecticide, anti-inflammatory, and central nervous system depressant<br> | Applications: Insecticide, anti-inflammatory, and central nervous system depressant<br> | ||
- | + | Plant Species: <i>Picea abies</i> (norway spruce) | |
+ | Synthase Gene: carene synthase (E.C. 4.2.3.107) | ||
</td> | </td> | ||
<td align="center" valign="center"> | <td align="center" valign="center"> | ||
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Main essential oils: Hops oil<br> | Main essential oils: Hops oil<br> | ||
Applications: Culinary spice, and anti-inflamitory<br> | Applications: Culinary spice, and anti-inflamitory<br> | ||
- | + | Plant Species: <i>Zingiber zerumbet</i> (shampoo ginger) | |
+ | Synthase Gene: α-humulene synthase (E.C. 4.2.3.104) | ||
</td> | </td> | ||
</tr> | </tr> | ||
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Main essential oils: Thyme and Hops oil <br> | Main essential oils: Thyme and Hops oil <br> | ||
Applications: Fragrance, analgesic, and anti-inflamitory<br> | Applications: Fragrance, analgesic, and anti-inflamitory<br> | ||
- | + | Plant Species: <i>Perilla frutescens</i> (Green Shiso) | |
+ | Synthase Gene: myrcene synthase (E.C. 4.2.3.15) | ||
</td> | </td> | ||
<td align="center" valign="center"> | <td align="center" valign="center"> | ||
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Main essential oils: Lavender oil<br> | Main essential oils: Lavender oil<br> | ||
Applications: Fragrance, and insecticide<br> | Applications: Fragrance, and insecticide<br> | ||
- | + | Plant Species: <i>Mentha citrata</i> (lemon mint) | |
+ | Synthase Gene: (R)-linalool synthase (E.C. 4.2.3.26) | ||
</td> | </td> | ||
<td align="center" valign="center"> | <td align="center" valign="center"> | ||
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Main essential oils: Citrus and Coriander oil<br> | Main essential oils: Citrus and Coriander oil<br> | ||
Applications: Fragrance, and insecticide<br> | Applications: Fragrance, and insecticide<br> | ||
- | + | Plant Species Chosen: <i>Arabidopsis thaliana</i> (thale cress) | |
+ | Synthase Gene: (S)-linalool synthase (E.C. 4.2.3.25) | ||
</td> | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td align="center" valign="center"> | <td align="center" valign="center"> | ||
- | <img src=" | + | <img src="https://static.igem.org/mediawiki/parts/e/e8/VU_Sabinene.png" width="200" /> |
<br /> | <br /> | ||
- | + | <b>Sabinene</b><br><br> | |
+ | Main essential oils: Junier Coriander oil<br> | ||
+ | Applications: Spice, and ntimicrobial<br> | ||
+ | Plant Species Chosen: <i> Salvia officinalis</i> (sage) | ||
+ | Synthase Gene: (+)-sabinene synthase (E.C. 4.2.3.110) | ||
</td> | </td> | ||
<td align="center" valign="center"> | <td align="center" valign="center"> | ||
- | <img src=" | + | <img src="https://static.igem.org/mediawiki/parts/5/54/VU_Santalene.png" width="200" /> |
<br /> | <br /> | ||
- | + | <b>Santalene</b><br><br> | |
+ | Main essential oils: Sandalwood oil<br> | ||
+ | Applications: Fragrance, antiviral, and tumor-suppressant<br> | ||
+ | Plant Species Chosen: <i> Santalum album</i> (sandalwood tree) | ||
+ | Synthase Gene: α-santalene synthase (E.C. 4.2.3.82) | ||
</td> | </td> | ||
<td align="center" valign="center"> | <td align="center" valign="center"> | ||
- | <img src=" | + | <img src="https://static.igem.org/mediawiki/parts/8/8d/VU_Zingiberene.png" width="200" /> |
<br /> | <br /> | ||
- | + | <b>Zingiberene</b><br><br> | |
+ | Main essential oils: Ginger oil<br> | ||
+ | Applications: Flavoring and pesticide<br> | ||
+ | Plant Species Chosen: <i> Ocimum basilicum</i> (basil) | ||
+ | Synthase Gene: α-zingiberene synthase (E.C. 4.2.3.65) | ||
</td> | </td> | ||
</tr> | </tr> | ||
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<p> <font size="3" face="georgia"> | <p> <font size="3" face="georgia"> | ||
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+ | MOVE ALL RESULTS in terp table to table with column checking if plant successfully grown, genomic DNA successfully extracted, synthase gene successfully PCR isolated, plant RNA successfully extracted, synthase gene cDNA successfully reverse transcribed, gene successfully mutagenized, gene successfully expressed in E. coli or Yeast | ||
<figure> | <figure> |
Revision as of 02:05, 16 October 2014
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Introduction
The production of plant essential oils and their derivatives represents an over 9 billion dollar industry when considering just their applications in the food and fragrance industries 1. A staggering 23 million kilograms of citrus oil alone are produced worldwide each year. Up until only a couple decades ago, the production of these essential oils was done exclusively by chemical extraction from plant material. However, the sudden emergence of synthetic biology a versatile and efficient tool has the potential to transform this immense industry, the products of which nearly everyone will come in contact with on a daily basis.
References: 1. USDA Industrial Uses Reports. Essential Oils Widely Used in Flavors and Fragrances. September 1995. 2. Ajikumar PK, Tyo K, Carlsen S, Mucha O, Phon TH, Stephanopoulos G. Terpenoids: opportunities for biosynthesis of natural product drugs using engineered microorganisms. Mol Pharm. 2008;5(2):167-90. |
Project Goals
MOVE ALL RESULTS in terp table to table with column checking if plant successfully grown, genomic DNA successfully extracted, synthase gene successfully PCR isolated, plant RNA successfully extracted, synthase gene cDNA successfully reverse transcribed, gene successfully mutagenized, gene successfully expressed in E. coli or Yeast
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