Team:Vanderbilt/Parts

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<h3> BBa_K1322231- Optimized Santalene Synthase</h3>
<h3> BBa_K1322231- Optimized Santalene Synthase</h3>
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BBa_K1322231 is a codon-optimized biobrick part encoding the gene for alpha-santalene synthase (EC 4.2.3.82). The enzyme catalyze the conversion of the common isoprenoid intermediate farnesyl pyrophosphate (FPP) into the sesquiterpene (+)-alpha-santelene in a single step. Traces of (-)-beta-santalene and bergamontene have previously been shown to be produced by this enzyme as well.
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<br><br>
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The gene, derived from a relative of the exotic sandalwood tree, has been demonstrated to produce functional terpene product in both yeast (Scalcinati et al 2012) and E. coli (data pending). This is possible due to several endogenous pathways that produce FPP as an intermediate, including the MEV and MEP pathways,
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<img src="https://static.igem.org/mediawiki/parts/0/0b/VU_San_psb_confirmation.jpg" align=right  width="300"  style="padding-bottom:0.5em; float:right" />
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In addition to being a prized fragrance, with what is often described as a warm, sweet woody scent, the sandalwood oil has been investigated for a number of other practical applications, including as a chemoprotective against carcinogenesis (Banaerjee, Ecavade, and Rao 1993) and inhibitors of viral reproduction (Koch et al 2008).
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We confirmed our part succesfully ligated into pSB1C3 by a diagnostic digest of miniprepped plasmid.
<h3> pVU14004 </h3>
<h3> pVU14004 </h3>
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<h3> Sabinene Synthase </h3>
<h3> Sabinene Synthase </h3>
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References: <br>Scalcinati et al.: Combined metabolic engineering of precursor and co-factor supply to increase α-santalene production by Saccharomyces cerevisiae. Microbial Cell Factories 2012 11:117<br>
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. Banerjee, Ecavade and Rao: Modulatory influence of sandalwood oil on mouse hepatic glutathione S-transferase activity and acid soluble sulphydryl level. Cancer Letters, 68 (1993) 105 - 109
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Koch et al: Inhibitory effect of essential oils against herpes simplex virus type 2. Phytomedicine 2008;15(1-2):71-8. <br>
BBa_K1322231
BBa_K1322231
BBa_K1322001
BBa_K1322001

Revision as of 00:35, 17 October 2014

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BBa_K1322231- Optimized Santalene Synthase

BBa_K1322231 is a codon-optimized biobrick part encoding the gene for alpha-santalene synthase (EC 4.2.3.82). The enzyme catalyze the conversion of the common isoprenoid intermediate farnesyl pyrophosphate (FPP) into the sesquiterpene (+)-alpha-santelene in a single step. Traces of (-)-beta-santalene and bergamontene have previously been shown to be produced by this enzyme as well.

The gene, derived from a relative of the exotic sandalwood tree, has been demonstrated to produce functional terpene product in both yeast (Scalcinati et al 2012) and E. coli (data pending). This is possible due to several endogenous pathways that produce FPP as an intermediate, including the MEV and MEP pathways,

In addition to being a prized fragrance, with what is often described as a warm, sweet woody scent, the sandalwood oil has been investigated for a number of other practical applications, including as a chemoprotective against carcinogenesis (Banaerjee, Ecavade, and Rao 1993) and inhibitors of viral reproduction (Koch et al 2008).

We confirmed our part succesfully ligated into pSB1C3 by a diagnostic digest of miniprepped plasmid.

pVU14004

As a shuttle vector, pVU14006 is capable of expression both in E. coli and S. cerevisiae. It has .... pVU14006

BBa_K1322001- All-RFC Compatible Fluorescent Oscillator

Sabinene Synthase



References:
Scalcinati et al.: Combined metabolic engineering of precursor and co-factor supply to increase α-santalene production by Saccharomyces cerevisiae. Microbial Cell Factories 2012 11:117
. Banerjee, Ecavade and Rao: Modulatory influence of sandalwood oil on mouse hepatic glutathione S-transferase activity and acid soluble sulphydryl level. Cancer Letters, 68 (1993) 105 - 109 Koch et al: Inhibitory effect of essential oils against herpes simplex virus type 2. Phytomedicine 2008;15(1-2):71-8.
BBa_K1322231 BBa_K1322001