Team:Vanderbilt/Notebook

From 2014.igem.org

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<div style="color:black;font-size:12pt">'''March 27th'''</div>
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<div style="color:black;font-size:11pt">'''March 27th'''</div>
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* Extracted genomic DNA from 100 mg of <i>Arabidopsis thalina </i> leaves using a Viogene DNA extraction kit
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* Extracted genomic DNA from 100 mg of <i>Arabidopsis thalina </i> leaves using a Viogene DNA extraction kit. Samples were labeled ZIN for zingiberene.
* Two samples were prepared and nanodropped. The concentration of the first was 2.6 ng/ul, and the second was 3.5 ng/ul of DNA, indicating a minimal yield of DNA.  
* Two samples were prepared and nanodropped. The concentration of the first was 2.6 ng/ul, and the second was 3.5 ng/ul of DNA, indicating a minimal yield of DNA.  
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<div style="color:black;font-size:11pt">'''March 30th'''</div>
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* Extracted genomic DNA from 100 mg of <i> Picea abies </i> needles using the same extraction kit and protocol. Samples were labeled CAR for carene.
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* Two samples were prepared and nanodropped. The concentration of the first was 0.9 ng/ul, and the second was 3.5 ng/ul of DNA, indicating a minimal yield of DNA.
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<div style="color:black;font-size:11pt">'''March 31th'''</div>
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* Ran a 0.6 % argarose gel on the DNA extracted from ZIN and CAR, as well as the column flow-through from the kit.
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* ZIN1, ZIN2, CAR1, and CAR2 all show a distinct DNA band above the highest rung on the DNA ladder (>10kb), showing the presence of DNA. No bands were seen on the kit flow-through. Indicates successful genomic extraction.
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<div style="color:black;font-size:12pt">'''August 24th'''</div>
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<div style="color:black;font-size:11pt">'''August 24th'''</div>
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<div style="color:black;font-size:11pt">'''August 24th'''</div>
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Revision as of 22:04, 4 October 2014


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Lab Notebook



Spring 2014

March 27th
  • Extracted genomic DNA from 100 mg of Arabidopsis thalina leaves using a Viogene DNA extraction kit. Samples were labeled ZIN for zingiberene.
  • Two samples were prepared and nanodropped. The concentration of the first was 2.6 ng/ul, and the second was 3.5 ng/ul of DNA, indicating a minimal yield of DNA.
March 30th
  • Extracted genomic DNA from 100 mg of Picea abies needles using the same extraction kit and protocol. Samples were labeled CAR for carene.
  • Two samples were prepared and nanodropped. The concentration of the first was 0.9 ng/ul, and the second was 3.5 ng/ul of DNA, indicating a minimal yield of DNA.
March 31th
  • Ran a 0.6 % argarose gel on the DNA extracted from ZIN and CAR, as well as the column flow-through from the kit.
  • ZIN1, ZIN2, CAR1, and CAR2 all show a distinct DNA band above the highest rung on the DNA ladder (>10kb), showing the presence of DNA. No bands were seen on the kit flow-through. Indicates successful genomic extraction.


Summer 2014

August 24th
  • Put text here


Fall 2014

August 24th
  • Put text here
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