Team:Valencia UPV/VUPV Parts

From 2014.igem.org

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<p>Four of them could be sent thanks to the collaboration from the <a class="normal-link-page" href="http://2014.igem.org/Team:NRP-UEA-Norwich">NRP-UEA-Norwich</a> team, who very kindly provided us with their MoFlippers. These were the parts coding sequences for <a class="normal-link-page" href="http://parts.igem.org/Part:BBa_K1554001">AtrΔ11</a>, <a class="normal-link-page" href="http://parts.igem.org/Part:BBa_K1554002">HarFAR</a> and <a class="normal-link-page" href="http://parts.igem.org/Part:BBa_K1554003">EaDAcT</a> and the <a class="normal-link-page" href="http://parts.igem.org/Part:BBa_K1554000">TA29</a> promoter.</p><br/>
<p>Four of them could be sent thanks to the collaboration from the <a class="normal-link-page" href="http://2014.igem.org/Team:NRP-UEA-Norwich">NRP-UEA-Norwich</a> team, who very kindly provided us with their MoFlippers. These were the parts coding sequences for <a class="normal-link-page" href="http://parts.igem.org/Part:BBa_K1554001">AtrΔ11</a>, <a class="normal-link-page" href="http://parts.igem.org/Part:BBa_K1554002">HarFAR</a> and <a class="normal-link-page" href="http://parts.igem.org/Part:BBa_K1554003">EaDAcT</a> and the <a class="normal-link-page" href="http://parts.igem.org/Part:BBa_K1554000">TA29</a> promoter.</p><br/>
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<p>We designed other vector which can be used to assemble two transcriptional units in alpha. It has two BsmBI sites which leave overhangs that match with GoldenBraid 2.0 grammar. We call them <a class="normal-link-page" href="http://parts.igem.org/Part:BBa_K1554006">"omega undercover"</a> as it works in a similar way of the GoldenBraid 2.0 omega vector but with a pSB1A3 backbone.</p><br/>
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<p>We also designed a vector that has de backbone of the Biobrick vector pSB1A3 but compatible with GoldenBraid 2.0. It contains two BsmBI restriction sites which leave the required overhangs to perform a GoldenBraid ligation of two transcriptional units in alpha vectors. We call it <a class="normal-link-page" href="http://parts.igem.org/Part:BBa_K1554006">"omega undercover"</a> as it works in a similar way of the GoldenBraid 2.0 omega vector but with a pSB1A3 backbone.</p><br/>
<div align="center"><img src="http://2014.igem.org/wiki/images/4/45/VUPVomega_undercover.jpg" alt="omega_undercover"></img></div><br/>
<div align="center"><img src="http://2014.igem.org/wiki/images/4/45/VUPVomega_undercover.jpg" alt="omega_undercover"></img></div><br/>
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<p>Using the omega undercover plasmid we constructed the two biosafety devices which consisted on an identity preservation module, expressing either <a class="normal-link-page" href="http://parts.igem.org/Part:BBa_K1554004">yellow</a> or <a class="normal-link-page" href="http://parts.igem.org/Part:BBa_K1554005">blue</a> chromoprotein, and a male sterility module.</p><br/>
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<p>Using the omega undercover plasmid, we constructed the two biosafety devices which consist on an identity preservation module, expressing either <a class="normal-link-page" href="http://parts.igem.org/Part:BBa_K1554004">yellow</a> or <a class="normal-link-page" href="http://parts.igem.org/Part:BBa_K1554005">blue</a> chromoprotein, and a male sterility module.</p><br/>
<div align="center"><img src="http://2014.igem.org/wiki/images/e/e2/VUPV_biosafety_constr_omega_undercover.jpg" alt="omega_undercover2"></img></div><br/><br/><br/><br/>
<div align="center"><img src="http://2014.igem.org/wiki/images/e/e2/VUPV_biosafety_constr_omega_undercover.jpg" alt="omega_undercover2"></img></div><br/><br/><br/><br/>

Revision as of 17:52, 15 October 2014

Achievements > Parts


Parts

Our team sent 7 BioBrick Parts to the Registry.


Four of them could be sent thanks to the collaboration from the NRP-UEA-Norwich team, who very kindly provided us with their MoFlippers. These were the parts coding sequences for AtrΔ11, HarFAR and EaDAcT and the TA29 promoter.


We also designed a vector that has de backbone of the Biobrick vector pSB1A3 but compatible with GoldenBraid 2.0. It contains two BsmBI restriction sites which leave the required overhangs to perform a GoldenBraid ligation of two transcriptional units in alpha vectors. We call it "omega undercover" as it works in a similar way of the GoldenBraid 2.0 omega vector but with a pSB1A3 backbone.


omega_undercover

Using the omega undercover plasmid, we constructed the two biosafety devices which consist on an identity preservation module, expressing either yellow or blue chromoprotein, and a male sterility module.


omega_undercover2




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