http://2014.igem.org/wiki/index.php?title=Team:Valencia_UPV/Project/modules/release&feed=atom&action=historyTeam:Valencia UPV/Project/modules/release - Revision history2024-03-29T02:24:53ZRevision history for this page on the wikiMediaWiki 1.16.5http://2014.igem.org/wiki/index.php?title=Team:Valencia_UPV/Project/modules/release&diff=397429&oldid=prevAlrual at 03:28, 18 October 20142014-10-18T03:28:50Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>Specific expression of sex pheromone <a class="normal-link-page" href="https://2014.igem.org/Team:Valencia_UPV/Project/modules/biosynthesis">biosynthetic pathways</a> in glandular trichomes also decreased the disturbance in the plant’s metabolism. We accomplished specific expression in glandular trichomes under the regulation of the promoter PCPS2, from Nicotiana tabacum. PCPS2 naturally regulates transcription of a terpene synthase in glandular trichome cells with great specificity [3]. Expression in glandular trichomes is an elegant strategy to release pheromones into the air causing the minimum metabolic impact in the plant.</p><br/><br/><br/><br/></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>Specific expression of sex pheromone <a class="normal-link-page" href="https://2014.igem.org/Team:Valencia_UPV/Project/modules/biosynthesis">biosynthetic pathways</a> in glandular trichomes also decreased the disturbance in the plant’s metabolism. We accomplished specific expression in glandular trichomes under the regulation of the promoter PCPS2, from <ins class="diffchange diffchange-inline"><i></ins>Nicotiana tabacum<ins class="diffchange diffchange-inline"></i></ins>. PCPS2 naturally regulates transcription of a terpene synthase in glandular trichome cells with great specificity [3]. Expression in glandular trichomes is an elegant strategy to release pheromones into the air causing the minimum metabolic impact in the plant.</p><br/><br/><br/><br/></div></td></tr>
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</table>Alrualhttp://2014.igem.org/wiki/index.php?title=Team:Valencia_UPV/Project/modules/release&diff=229496&oldid=prevIvllobel at 15:28, 14 October 20142014-10-14T15:28:12Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>Specific expression of sex pheromone <a class="normal-link-page" href="https://2014.igem.org/Team:Valencia_UPV/Project/modules/biosynthesis">biosynthetic pathways</a> in glandular trichomes also decreased the disturbance in the plant’s metabolism. We accomplished specific expression in glandular trichomes under the regulation of the promoter PCPS2, from Nicotiana tabacum. PCPS2 naturally regulates transcription of a terpene synthase in glandular trichome cells with great specificity [3]. Expression in glandular trichomes is an elegant strategy to release pheromones into the air causing the minimum metabolic impact in the plant.</p><br/><br/><br/></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>Specific expression of sex pheromone <a class="normal-link-page" href="https://2014.igem.org/Team:Valencia_UPV/Project/modules/biosynthesis">biosynthetic pathways</a> in glandular trichomes also decreased the disturbance in the plant’s metabolism. We accomplished specific expression in glandular trichomes under the regulation of the promoter PCPS2, from Nicotiana tabacum. PCPS2 naturally regulates transcription of a terpene synthase in glandular trichome cells with great specificity [3]. Expression in glandular trichomes is an elegant strategy to release pheromones into the air causing the minimum metabolic impact in the plant.</p<ins class="diffchange diffchange-inline">><br/</ins>><br/><br/><br/></div></td></tr>
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</table>Ivllobelhttp://2014.igem.org/wiki/index.php?title=Team:Valencia_UPV/Project/modules/release&diff=229492&oldid=prevIvllobel at 15:28, 14 October 20142014-10-14T15:28:01Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>Specific expression of sex pheromone <a class="normal-link-page" href="https://2014.igem.org/Team:Valencia_UPV/Project/modules/biosynthesis">biosynthetic pathways</a> in glandular trichomes also decreased the disturbance in the plant’s metabolism. We accomplished specific expression in glandular trichomes under the regulation of the promoter PCPS2, from Nicotiana tabacum. PCPS2 naturally regulates transcription of a terpene synthase in glandular trichome cells with great specificity [3]. Expression in glandular trichomes is an elegant strategy to release pheromones into the air causing the minimum metabolic impact in the plant.</p><br/><br/></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>Specific expression of sex pheromone <a class="normal-link-page" href="https://2014.igem.org/Team:Valencia_UPV/Project/modules/biosynthesis">biosynthetic pathways</a> in glandular trichomes also decreased the disturbance in the plant’s metabolism. We accomplished specific expression in glandular trichomes under the regulation of the promoter PCPS2, from Nicotiana tabacum. PCPS2 naturally regulates transcription of a terpene synthase in glandular trichome cells with great specificity [3]. Expression in glandular trichomes is an elegant strategy to release pheromones into the air causing the minimum metabolic impact in the plant.</p<ins class="diffchange diffchange-inline">><br/</ins>><br/><br/></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p align="center"><strong>References</strong></p><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p align="center"><strong>References</strong></p><br/></div></td></tr>
</table>Ivllobelhttp://2014.igem.org/wiki/index.php?title=Team:Valencia_UPV/Project/modules/release&diff=228610&oldid=prevIvllobel at 14:40, 14 October 20142014-10-14T14:40:31Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p><h3 class="hook" align="left"><a>Project</a> > <a href="https://2014.igem.org/Team:Valencia_UPV/Project/modules">Modules</a> > <a>Pheromone Release</a> </h3></p><br/><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p><h3 class="hook" align="left"><a>Project</a> > <a href="https://2014.igem.org/Team:Valencia_UPV/Project/modules">Modules</a> > <a>Pheromone Release</a> </h3></p><br/><br/></div></td></tr>
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</table>Ivllobelhttp://2014.igem.org/wiki/index.php?title=Team:Valencia_UPV/Project/modules/release&diff=228609&oldid=prevIvllobel at 14:40, 14 October 20142014-10-14T14:40:23Z<p></p>
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</table>Ivllobelhttp://2014.igem.org/wiki/index.php?title=Team:Valencia_UPV/Project/modules/release&diff=228607&oldid=prevIvllobel at 14:40, 14 October 20142014-10-14T14:40:11Z<p></p>
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</table>Ivllobelhttp://2014.igem.org/wiki/index.php?title=Team:Valencia_UPV/Project/modules/release&diff=228571&oldid=prevIvllobel at 14:37, 14 October 20142014-10-14T14:37:48Z<p></p>
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</table>Ivllobelhttp://2014.igem.org/wiki/index.php?title=Team:Valencia_UPV/Project/modules/release&diff=228567&oldid=prevIvllobel at 14:37, 14 October 20142014-10-14T14:37:30Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Specific expression of sex pheromone <a class="normal-link-page" href="https://2014.igem.org/Team:Valencia_UPV/Project/modules/biosynthesis">biosynthetic pathways</a> in glandular trichomes also decreased the disturbance in the plant’s metabolism. We accomplished specific expression in glandular trichomes under the regulation of the promoter PCPS2, from Nicotiana tabacum. PCPS2 naturally regulates transcription of a terpene synthase in glandular trichome cells with great specificity [3]. Expression in glandular trichomes is an elegant strategy to release pheromones into the air causing the minimum metabolic impact in the plant.</p><br/><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Specific expression of sex pheromone <a class="normal-link-page" href="https://2014.igem.org/Team:Valencia_UPV/Project/modules/biosynthesis">biosynthetic pathways</a> in glandular trichomes also decreased the disturbance in the plant’s metabolism. We accomplished specific expression in glandular trichomes under the regulation of the promoter PCPS2, from Nicotiana tabacum. PCPS2 naturally regulates transcription of a terpene synthase in glandular trichome cells with great specificity [3]. Expression in glandular trichomes is an elegant strategy to release pheromones into the air causing the minimum metabolic impact in the plant.</p><br/><br/></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p align="center"><strong>References</strong></p><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p align="center"><strong>References</strong></p><br/></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li>3. Sallaud C, Giacalone C, Topfer R, Goepfert S, Bakaher N, et al. (2012) Characterization of two genes for the biosynthesis of the labdane diterpene Z-abienol in tobacco (Nicotiana tabacum) glandular trichomes. Plant J 72: 1-17.</li> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li>3. Sallaud C, Giacalone C, Topfer R, Goepfert S, Bakaher N, et al. (2012) Characterization of two genes for the biosynthesis of the labdane diterpene Z-abienol in tobacco (Nicotiana tabacum) glandular trichomes. Plant J 72: 1-17.</li> </div></td></tr>
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</table>Ivllobelhttp://2014.igem.org/wiki/index.php?title=Team:Valencia_UPV/Project/modules/release&diff=228534&oldid=prevIvllobel at 14:36, 14 October 20142014-10-14T14:36:04Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>Specific expression of sex pheromone <a class="normal<del class="diffchange diffchange-inline">-page</del>-link" href="https://2014.igem.org/Team:Valencia_UPV/Project/modules/biosynthesis">biosynthetic pathways</a> in glandular trichomes also decreased the disturbance in the plant’s metabolism. We accomplished specific expression in glandular trichomes under the regulation of the promoter PCPS2, from Nicotiana tabacum. PCPS2 naturally regulates transcription of a terpene synthase in glandular trichome cells with great specificity [3]. Expression in glandular trichomes is an elegant strategy to release pheromones into the air causing the minimum metabolic impact in the plant.</p><br/><br/></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>Specific expression of sex pheromone <a class="normal-link<ins class="diffchange diffchange-inline">-page</ins>" href="https://2014.igem.org/Team:Valencia_UPV/Project/modules/biosynthesis">biosynthetic pathways</a> in glandular trichomes also decreased the disturbance in the plant’s metabolism. We accomplished specific expression in glandular trichomes under the regulation of the promoter PCPS2, from Nicotiana tabacum. PCPS2 naturally regulates transcription of a terpene synthase in glandular trichome cells with great specificity [3]. Expression in glandular trichomes is an elegant strategy to release pheromones into the air causing the minimum metabolic impact in the plant.</p><br/><br/></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p align="center"><strong>References</strong></p><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p align="center"><strong>References</strong></p><br/></div></td></tr>
</table>Ivllobelhttp://2014.igem.org/wiki/index.php?title=Team:Valencia_UPV/Project/modules/release&diff=228529&oldid=prevIvllobel at 14:35, 14 October 20142014-10-14T14:35:49Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>We use sex pheromones to avoid sexual encounter in a strategy called mating disruption. However, producing the pheromone is not enough for this strategy to be effective, the pheromone must be released into the air. Sex pheromones concentration in the air has to reach high enough levels to cause mating disruption.</p><br/><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>We use sex pheromones to avoid sexual encounter in a strategy called mating disruption. However, producing the pheromone is not enough for this strategy to be effective, the pheromone must be released into the air. Sex pheromones concentration in the air has to reach high enough levels to cause mating disruption.</p><br/><br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>Even though pheromones are volatile compound, they still need to find a way out of the plant. For that reason, we decided to produce pheromones in epidermal organs called glandular trichomes to release volatile compounds into the air [1]. These organs are located in the surface of aerial parts of many higher plant species and contain higher concentration of secondary metabolites than an average cell in the plant (up to 15% of dry weight) [2] (Structure in figure 1). This characteristics make glandular trichomes great organs to release volatile compounds.</p><br/><br/></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>Even though pheromones are volatile compound, they still need to find a way out of the plant. For that reason, we decided to produce pheromones in epidermal organs called <ins class="diffchange diffchange-inline"><span class="black-bold"></ins>glandular trichomes<ins class="diffchange diffchange-inline"></span> </ins>to release volatile compounds into the air [1]. These organs are located in the surface of aerial parts of many higher plant species and contain higher concentration of secondary metabolites than an average cell in the plant (up to 15% of dry weight) [2] (Structure in figure 1). This characteristics make glandular trichomes great organs to release volatile compounds.</p><br/><br/></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>Specific expression of sex pheromone biosynthetic pathways in glandular trichomes also decreased the disturbance in the plant’s metabolism. We accomplished specific expression in glandular trichomes under the regulation of the promoter PCPS2, from Nicotiana tabacum. PCPS2 naturally regulates transcription of a terpene synthase in glandular trichome cells with great specificity [3]. Expression in glandular trichomes is an elegant strategy to release pheromones into the air causing the minimum metabolic impact in the plant.</p><br/><br/></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>Specific expression of sex pheromone <ins class="diffchange diffchange-inline"><a class="normal-page-link" href="https://2014.igem.org/Team:Valencia_UPV/Project/modules/biosynthesis"></ins>biosynthetic pathways<ins class="diffchange diffchange-inline"></a> </ins> in glandular trichomes also decreased the disturbance in the plant’s metabolism. We accomplished specific expression in glandular trichomes under the regulation of the promoter PCPS2, from Nicotiana tabacum. PCPS2 naturally regulates transcription of a terpene synthase in glandular trichome cells with great specificity [3]. Expression in glandular trichomes is an elegant strategy to release pheromones into the air causing the minimum metabolic impact in the plant.</p><br/><br/></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p align="center"><strong>References</strong></p><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p align="center"><strong>References</strong></p><br/></div></td></tr>
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