http://2014.igem.org/wiki/index.php?title=Team:Valencia_UPV/Project/modules/methodology/parts_construction&feed=atom&action=historyTeam:Valencia UPV/Project/modules/methodology/parts construction - Revision history2024-03-29T08:23:55ZRevision history for this page on the wikiMediaWiki 1.16.5http://2014.igem.org/wiki/index.php?title=Team:Valencia_UPV/Project/modules/methodology/parts_construction&diff=388234&oldid=prevIvllobel at 02:21, 18 October 20142014-10-18T02:21:50Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p><h3 class="hook" align="left"><a>Project</a> > <a href="https://2014.igem.org/Team:Valencia_UPV/Project/modules">Modules</a> > <a href="https://2014.igem.org/Team:Valencia_UPV/Project/modules/methodology">Methodology<del class="diffchange diffchange-inline"></a> > <a href="https://2014.igem.org/Team:Valencia_UPV/Project/modules/methodology#cloning">Cloning</del></a> > <a>Parts Construction</a></h3></p><br/><br/></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p><h3 class="hook" align="left"><a>Project</a> > <a href="https://2014.igem.org/Team:Valencia_UPV/Project/modules">Modules</a> > <a href="https://2014.igem.org/Team:Valencia_UPV/Project/modules/methodology">Methodology</a> > <a>Parts Construction</a></h3></p><br/><br/></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div align="center"><span class="coda"><roja>P</roja>arts <roja>C</roja>onstruction</span> </div><br/><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div align="center"><span class="coda"><roja>P</roja>arts <roja>C</roja>onstruction</span> </div><br/><br/></div></td></tr>
</table>Ivllobelhttp://2014.igem.org/wiki/index.php?title=Team:Valencia_UPV/Project/modules/methodology/parts_construction&diff=377478&oldid=prevIvllobel at 00:47, 18 October 20142014-10-18T00:47:37Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div align="center"><img src="https://static.igem.org/mediawiki/2014/e/e2/VUPV_biosafety_constr_omega_undercover.jpg" width="450px"></img></div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div align="center"><img src="https://static.igem.org/mediawiki/2014/e/e2/VUPV_biosafety_constr_omega_undercover.jpg" width="450px"></img></div><br/></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <div align="center"><p style="text-align: center; font-style: italic; font-size: 0.8em; width: 700px;"><span class="black-bold">Figure 2</span>.Construction of parts/devices <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1554004" class="normal-link-page">BBa_K1554004</a> and <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1554005" class="normal-link-page">BBa_K1554005</a>.</p></div><br/></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <div align="center"><p style="text-align: center; font-style: italic; font-size: 0.8em; width: 700px;"><span class="black-bold">Figure 2</span>.Construction of parts/devices <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1554004" class="normal-link-page">BBa_K1554004</a> and <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1554005" class="normal-link-page">BBa_K1554005</a>.</p></div<ins class="diffchange diffchange-inline">><br/></br></br></br</ins>><br/></div></td></tr>
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</table>Ivllobelhttp://2014.igem.org/wiki/index.php?title=Team:Valencia_UPV/Project/modules/methodology/parts_construction&diff=377422&oldid=prevIvllobel at 00:47, 18 October 20142014-10-18T00:47:12Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div align="center"><p style="text-align: center; font-style: italic; font-size: 0.8em; width: 700px;"><span class="black-bold">Figure 2</span>.Construction of parts/devices <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1554004" class="normal-link-page">BBa_K1554004</a> and <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1554005" class="normal-link-page">BBa_K1554005</a>.</p></div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div align="center"><p style="text-align: center; font-style: italic; font-size: 0.8em; width: 700px;"><span class="black-bold">Figure 2</span>.Construction of parts/devices <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1554004" class="normal-link-page">BBa_K1554004</a> and <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1554005" class="normal-link-page">BBa_K1554005</a>.</p></div><br/></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><a class="button-content" id="goto-left" align="center" href="https://2014.igem.org/Team:Valencia_UPV/Project/modules/methodology/flowchart"><strong>&larr; Go to Flowchart</strong></a></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><a class="button-content" id="goto-middle" align="center" href="https://2014.igem.org/Team:Valencia_UPV/Project/modules/methodology"><strong>Go to Methodology</strong></a></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><a class="button-content" id="goto-right" align="center" href="https://2014.igem.org/Team:Valencia_UPV/Project/modules/methodology/expression"><strong>Go to Transient Gene Expression &rarr;</strong></a></div></br></br></br><br/></ins></div></td></tr>
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</table>Ivllobelhttp://2014.igem.org/wiki/index.php?title=Team:Valencia_UPV/Project/modules/methodology/parts_construction&diff=360186&oldid=prevIvllobel at 22:10, 17 October 20142014-10-17T22:10:21Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p><h3 class="hook" align="left"><a>Project</a> > <a href="https://2014.igem.org/Team:Valencia_UPV/Project/modules">Modules</a> > <a href="https://2014.igem.org/Team:Valencia_UPV/Project/modules/methodology">Methodology</a> > <a href="https://2014.igem.org/Team:Valencia_UPV/Project/modules/methodology#cloning"><del class="diffchange diffchange-inline">Parts Construction</del></a> > <a><del class="diffchange diffchange-inline">Flowchart</del></a></h3></p><br/><br/></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p><h3 class="hook" align="left"><a>Project</a> > <a href="https://2014.igem.org/Team:Valencia_UPV/Project/modules">Modules</a> > <a href="https://2014.igem.org/Team:Valencia_UPV/Project/modules/methodology">Methodology</a> > <a href="https://2014.igem.org/Team:Valencia_UPV/Project/modules/methodology#cloning"><ins class="diffchange diffchange-inline">Cloning</ins></a> > <a><ins class="diffchange diffchange-inline">Parts Construction</ins></a></h3></p><br/><br/></div></td></tr>
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</table>Ivllobelhttp://2014.igem.org/wiki/index.php?title=Team:Valencia_UPV/Project/modules/methodology/parts_construction&diff=360152&oldid=prevIvllobel at 22:10, 17 October 20142014-10-17T22:10:00Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p><h3 class="hook" align="left"><a>Project</a> > <a><del class="diffchange diffchange-inline">Overview</del></a></h3></p><br/></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p><h3 class="hook" align="left"><a>Project</a> > <a <ins class="diffchange diffchange-inline">href="https://2014.igem.org/Team:Valencia_UPV/Project/modules"</ins>><ins class="diffchange diffchange-inline">Modules</a> > <a href="https://2014.igem.org/Team:Valencia_UPV/Project/modules/methodology">Methodology</a> > <a href="https://2014.igem.org/Team:Valencia_UPV/Project/modules/methodology#cloning">Parts Construction</a> > <a>Flowchart</ins></a></h3></p<ins class="diffchange diffchange-inline">><br/</ins>><br/></div></td></tr>
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</table>Ivllobelhttp://2014.igem.org/wiki/index.php?title=Team:Valencia_UPV/Project/modules/methodology/parts_construction&diff=359914&oldid=prevIvllobel at 22:07, 17 October 20142014-10-17T22:07:38Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>In addition, we designed a new vector that can be used to convert GoldenBraid multigenic assemblies into the BioBrick standard. This new vector is a modified pSB1A3 plasmid with two BsmBI recognition sites leaving upon BsmBI digestion overhangs that match the GoldenBraid 2.0 grammar (Figure 1). We called this new vector "omega undercover" as it works as the GoldenBraid 2.0 omega vector with a pSB1A3 backbone. This vector was sent to the Registry, so it can be used for future teams using Golden Gate based cloning strategies.</p><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>In addition, we designed a new vector that can be used to convert GoldenBraid multigenic assemblies into the BioBrick standard. This new vector is a modified pSB1A3 plasmid with two BsmBI recognition sites leaving upon BsmBI digestion overhangs that match the GoldenBraid 2.0 grammar (Figure 1). We called this new vector "omega undercover" as it works as the GoldenBraid 2.0 omega vector with a pSB1A3 backbone. This vector was sent to the Registry, so it can be used for future teams using Golden Gate based cloning strategies.</p><br/></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><div align="center"><img src="https://static.igem.org/mediawiki/2014/4/45/VUPVomega_undercover.jpg" alt="pheromone_pathway" title="Pheromone Pathway" width="450px"></img></div><br/></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> <div align="center"><p style="text-align: center; font-style: italic; font-size: 0.8em; width: 700px;"><span class="black-bold">Figure 1</span>."Omega undercover" vector.</p></div><br/></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Using the omega undercover plasmid we constructed the remaining 2 Parts that were sent to the Registry (Figure 2). These were two biosafety devices that consisted of two modules, a male sterility module (<a class="normal-link-page" href="https://2014.igem.org/Team:Valencia_UPV/Project/modules/biosafety">See Biosafety</a>) and an identity preservation module, expressing either <span class="blue-bold">yellow</span> or <span class="blue-bold">blue</span> chromoproteins.</p><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Using the omega undercover plasmid we constructed the remaining 2 Parts that were sent to the Registry (Figure 2). These were two biosafety devices that consisted of two modules, a male sterility module (<a class="normal-link-page" href="https://2014.igem.org/Team:Valencia_UPV/Project/modules/biosafety">See Biosafety</a>) and an identity preservation module, expressing either <span class="blue-bold">yellow</span> or <span class="blue-bold">blue</span> chromoproteins.</p><br/></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><div align="center"><img src="https://static.igem.org/mediawiki/2014/e/e2/VUPV_biosafety_constr_omega_undercover.jpg" width="450px"></img></div><br/></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> <div align="center"><p style="text-align: center; font-style: italic; font-size: 0.8em; width: 700px;"><span class="black-bold">Figure 2</span>.Construction of parts/devices <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1554004" class="normal-link-page">BBa_K1554004</a> and <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1554005" class="normal-link-page">BBa_K1554005</a>.</p></div><br/></ins></div></td></tr>
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</table>Ivllobelhttp://2014.igem.org/wiki/index.php?title=Team:Valencia_UPV/Project/modules/methodology/parts_construction&diff=359292&oldid=prevIvllobel at 22:01, 17 October 20142014-10-17T22:01:21Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p><h3 class="hook" align="left"><a>Project</a> > <a>Overview</a></h3></p><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p><h3 class="hook" align="left"><a>Project</a> > <a>Overview</a></h3></p><br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><div align="center"><span class="coda"><roja>P</roja><del class="diffchange diffchange-inline">roject </del><roja><del class="diffchange diffchange-inline">O</del></roja><del class="diffchange diffchange-inline">verview</del></span> </div><br/><br/></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><div align="center"><span class="coda"><roja>P</roja><ins class="diffchange diffchange-inline">arts </ins><roja><ins class="diffchange diffchange-inline">C</ins></roja><ins class="diffchange diffchange-inline">onstruction</ins></span> </div><br/><br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Our team used a Golden Gate based DNA cloning system, called GoldenBraid in order to construct the different DNA parts of our project. Unluckily, GoldenBraid 2.0 and BioBrick standard parts are different. The parts of the two systems have different flanking recognition sites and the overhangs obtained upon digestion are also different. Therefore, we needed a strategy to convert GoldenBraid standard parts to BioBricks.</p><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Our team used a Golden Gate based DNA cloning system, called GoldenBraid in order to construct the different DNA parts of our project. Unluckily, GoldenBraid 2.0 and BioBrick standard parts are different. The parts of the two systems have different flanking recognition sites and the overhangs obtained upon digestion are also different. Therefore, we needed a strategy to convert GoldenBraid standard parts to BioBricks.</p><br/></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>In addition, we designed a new vector that can be used to convert GoldenBraid multigenic assemblies into the BioBrick standard. This new vector is a modified pSB1A3 plasmid with two BsmBI recognition sites leaving upon BsmBI digestion overhangs that match the GoldenBraid 2.0 grammar (Figure 1). We called this new vector "omega undercover" as it works as the GoldenBraid 2.0 omega vector with a pSB1A3 backbone. This vector was sent to the Registry, so it can be used for future teams using Golden Gate based cloning strategies.</p><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>In addition, we designed a new vector that can be used to convert GoldenBraid multigenic assemblies into the BioBrick standard. This new vector is a modified pSB1A3 plasmid with two BsmBI recognition sites leaving upon BsmBI digestion overhangs that match the GoldenBraid 2.0 grammar (Figure 1). We called this new vector "omega undercover" as it works as the GoldenBraid 2.0 omega vector with a pSB1A3 backbone. This vector was sent to the Registry, so it can be used for future teams using Golden Gate based cloning strategies.</p><br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>Using the omega undercover plasmid we constructed the remaining 2 Parts that were sent to the Registry (Figure 2). These were two biosafety devices that consisted of two modules, a male sterility module (<del class="diffchange diffchange-inline">see </del>Biosafety) and an identity preservation module, expressing either yellow or blue chromoproteins.</p><br/></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>Using the omega undercover plasmid we constructed the remaining 2 Parts that were sent to the Registry (Figure 2). These were two biosafety devices that consisted of two modules, a male sterility module (<ins class="diffchange diffchange-inline"><a class="normal-link-page" href="https://2014.igem.org/Team:Valencia_UPV/Project/modules/biosafety">See </ins>Biosafety<ins class="diffchange diffchange-inline"></a></ins>) and an identity preservation module, expressing either <ins class="diffchange diffchange-inline"><span class="blue-bold"></ins>yellow<ins class="diffchange diffchange-inline"></span> </ins>or <ins class="diffchange diffchange-inline"><span class="blue-bold"></ins>blue<ins class="diffchange diffchange-inline"></span> </ins>chromoproteins.</p><br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td></tr>
</table>Ivllobelhttp://2014.igem.org/wiki/index.php?title=Team:Valencia_UPV/Project/modules/methodology/parts_construction&diff=359122&oldid=prevIvllobel: Created page with "{{:Team:Valencia_UPV/header}} <html> <div align="center"><div id="cn-box" align="justify"> <p><h3 class="hook" align="left"><a>Project</a> > <a>Overview</a></h3></p><br/> <div..."2014-10-17T21:59:28Z<p>Created page with "{{:Team:Valencia_UPV/header}} <html> <div align="center"><div id="cn-box" align="justify"> <p><h3 class="hook" align="left"><a>Project</a> > <a>Overview</a></h3></p><br/> <div..."</p>
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<p>Our team used a Golden Gate based DNA cloning system, called GoldenBraid in order to construct the different DNA parts of our project. Unluckily, GoldenBraid 2.0 and BioBrick standard parts are different. The parts of the two systems have different flanking recognition sites and the overhangs obtained upon digestion are also different. Therefore, we needed a strategy to convert GoldenBraid standard parts to BioBricks.</p><br/><br />
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<p>We sent 7 <a href="https://2014.igem.org/Team:Valencia_UPV/VUPV_Parts" class="normal-link-page">Parts</a> to the registry. Four of them, the coding sequences from <span class="blue-bold">AtrΔ11</span>, <span class="blue-bold">HarFAR</span> and <span class="blue-bold">EaDAcT</span> (Biosynthesis) and the <span class="blue-bold">TA29</span> promoter (Biosafety) were converted to the Biobrick standard in collaboration with the NRP-UEA-Norwich team, who very kindly provided us with their <a class="normal-link-page" href="https://2014.igem.org/Team:NRP-UEA-Norwich/Project_Mo-Flipper">Mo-Flippers</a>. These were modified pSB1C3 plasmids altered to include GoldenBraid restriction enzymes recognition sites.</p><br/><br />
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<p>In addition, we designed a new vector that can be used to convert GoldenBraid multigenic assemblies into the BioBrick standard. This new vector is a modified pSB1A3 plasmid with two BsmBI recognition sites leaving upon BsmBI digestion overhangs that match the GoldenBraid 2.0 grammar (Figure 1). We called this new vector "omega undercover" as it works as the GoldenBraid 2.0 omega vector with a pSB1A3 backbone. This vector was sent to the Registry, so it can be used for future teams using Golden Gate based cloning strategies.</p><br/><br />
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<p>Using the omega undercover plasmid we constructed the remaining 2 Parts that were sent to the Registry (Figure 2). These were two biosafety devices that consisted of two modules, a male sterility module (see Biosafety) and an identity preservation module, expressing either yellow or blue chromoproteins.</p><br/><br />
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