Team:Valencia UPV/Achievements

From 2014.igem.org

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<p>As it can bee observed surfing our wiki, at the end of this long road we have accomplished many positive results:</p>
<p>As it can bee observed surfing our wiki, at the end of this long road we have accomplished many positive results:</p>
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A plant able to produce three insect sexual pheromones from moths to produce insects mating disruption, the Sexy Plant. <a href="http://2014.igem.org/Team:Valencia_UPV/Project/results/pheromone_analysis" class="normal-link-page">Results: Pheromone analysis</a>
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<ul class="method">
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<li><a class="black-bold">A plant able to produce three insect sexual pheromones from moths to produce insects mating disruption, the Sexy Plant.:</a> <a href="http://2014.igem.org/Team:Valencia_UPV/Project/results/pheromone_analysis" class="normal-link-page">Results: Pheromone analysis</a></a>
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<ul>
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    <li>Obtained pheromones being among the most abundant plant organic volatile compounds.<span class="red-bold">(Z)-11-hexadecen-1-ol</span> is certainly the most abundant one.</li>
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    <li>Successful and functional assembly of each of the pheromone biosynthetic genes with the plant constitutive promoter P35S. Also multigenic assembly of all three transcription units in a single plasmid. <a href="http://2014.igem.org/Team:Valencia_UPV/Project/results/contructs" class="normal-link-page">Results: Constructions-Biosynthesis</a></li>
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    <li>Proof of our produced pheromones-insect interaction. <a href="http://2014.igem.org/Team:Valencia_UPV/Project/results/contructs" class="normal-link-page">Results: Electroantennography</a></li>
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</ul>
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</li>
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</p><br/><br/>
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<p>
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Obtained pheromones being among the most abundant plant organic volatile compounds.<span class="red-bold">(Z)-11-hexadecen-1-ol</span> is certainly the most abundant one.  
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</p>
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<ul class="method">
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<p>
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<li><a class="black-bold">A plant potentially able to release the produced pheromones into the environment.</a>
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Successful and functional assembly of each of the pheromone biosynthetic genes with the plant constitutive promoter P35S. Also multigenic assembly of all three transcription units in a single plasmid. <a href="http://2014.igem.org/Team:Valencia_UPV/Project/results/contructs" class="normal-link-page">Results: Constructions-Biosynthesis</a>
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</p>
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    <li>Successful cloning of a trichome-specific promoter (PCPS2) from the genome of N tabacum and subsequent assembly with GFP as a reporter. <a href="http://2014.igem.org/Team:Valencia_UPV/Project/results/constructs#tab2?display=block" class="normal-link-page">Results: Constructs-Pheromone release</a></li>
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Proof of our produced pheromones-insect interaction. <a href="http://2014.igem.org/Team:Valencia_UPV/Project/results/contructs" class="normal-link-page">Results: Electroantennography</a>
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    <li>Proof of the specificity of this promoter by GFP fluorescence detection. <a href="http://2014.igem.org/Team:Valencia_UPV/Project/results/trichome_expression" class="normal-link-page">Results: Trichome-specific expression</a></li>
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</p>
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    <li>Assembly of each gene of the pheromones production pathway with PCPS2 promoter and multigenic assembly with all three transcription units in a single plasmid. <a href="http://2014.igem.org/Team:Valencia_UPV/Project/results/constructs#release" class="normal-link-page">Results: Constructs-Pheromone release</a></a></li>
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</ul>
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</li>
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</p><br/><br/>
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<ul class="method">
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<li><a class="black-bold">A genetic switch able to control gene expression ready to be implemented in the plant. Results: Constructs-Switch</a>
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<ul>
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    <li>Cloning of the coding sequence from the CUP2 transcription factor from S cerevisiae and assembly with the CaMV constitutive promoter P35S. <a href="http://2014.igem.org/Team:Valencia_UPV/Project/results/constructs#switch" class="normal-link-page">Results: Construct-Switch</a></li>
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    <li>Creation of the Cupper-responsive chimeric promoter and assembly with Firefly luciferase gene as a reporter, P19 as a gene silencing suppressor, and Renilla luciferase as control for Luciferase expression assay.<a href="http://2014.igem.org/Team:Valencia_UPV/Project/results/constructs#switch" class="normal-link-page">Results: Construct-Switch</a></li>
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    <li>Multigenic assembly comprising the CUP2 transcriptional unit with the chimeric promoter and reporter gene assembly.
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<a href="http://2014.igem.org/Team:Valencia_UPV/Project/results/constructs#switch" class="normal-link-page">Results: Construct-Switch</a></li>
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</ul>
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</li>
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</p><br/><br/>
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<ul class="method">
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<li><a class="black-bold">A sterile and dark purple plant safe for living beings and the environment.<a href="http://2014.igem.org/Team:Valencia_UPV/Project/results/constructs#switch" class="normal-link-page">Biosafety</a>
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<ul>
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    <li>Multigenic assembly of two biosafety devices, comprising the Barnase (male-sterility) with one chromoprotein in each device, AmilCP or AmilGFP (identity preservation). <a href="http://2014.igem.org/Team:Valencia_UPV/Project/results/constructs#biosafety" class="normal-link-page">Results: Constructs-Biosafety</a></li>
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    <li>Purple plant to preserve its identity expressing ANT1 and JAF13 transcription factors. <a href="http://2014.igem.org/Team:Valencia_UPV/Project/results" class="normal-link-page">Results: Biosafety</a></li>
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</ul>
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</li>
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</p><br/><br/>
<p>
<p>
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A plant potentially able to release the produced pheromones into the environment.
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<a class="black-bold">Diffusion and communication of the project with involved experts and stakeholders.<a href="http://2014.igem.org/Team:Valencia_UPV/policy/activities" class="normal-link-page">Policy and Practices</a></a>  
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</p>
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<p>
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Successful cloning of a trichome-specific promoter (PCPS2) from the genome of N tabacum and subsequent assembly with GFP as a reporter. <a href="http://2014.igem.org/Team:Valencia_UPV/Project/results/constructs#tab2?display=block" class="normal-link-page">Results: Constructs-Pheromone release</a>
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</p>
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<p>
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Proof of the specificity of this promoter by GFP fluorescence detection.  <a href="http://2014.igem.org/Team:Valencia_UPV/Project/results/trichome_expression" class="normal-link-page">Results: Trichome-specific expression</a>
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</p>
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<p>
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Assembly of each gene of the pheromones production pathway with PCPS2 promoter and multigenic assembly with all three transcription units in a single plasmid. <a href="http://2014.igem.org/Team:Valencia_UPV/Project/results/constructs#release" class="normal-link-page">Results: Constructs-Pheromone release</a>
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</p>
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<br/><br/>
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<p>
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A genetic switch able to control gene expression ready to be implemented in the plant. Results: Constructs-Switch
+
-
</p>
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-
<p>
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Cloning of the coding sequence from the CUP2 transcription factor from S cerevisiae and assembly with the CaMV constitutive promoter P35S. <a href="http://2014.igem.org/Team:Valencia_UPV/Project/results/constructs#switch" class="normal-link-page">Results: Construct-Switch</a>
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</p>
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<p>
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Creation of the Cupper-responsive chimeric promoter and assembly with Firefly luciferase gene as a reporter, P19 as a gene silencing suppressor, and Renilla luciferase as control for Luciferase expression assay.<a href="http://2014.igem.org/Team:Valencia_UPV/Project/results/constructs#switch" class="normal-link-page">Results: Construct-Switch</a>
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</p>
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<p>
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Multigenic assembly comprising the CUP2 transcriptional unit with the chimeric promoter and reporter gene assembly.
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<a href="http://2014.igem.org/Team:Valencia_UPV/Project/results/constructs#switch" class="normal-link-page">Results: Construct-Switch</a>
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</p>
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<br/><br/>
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<p>
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A sterile and dark purple plant safe for living beings and the environment.<a href="http://2014.igem.org/Team:Valencia_UPV/Project/results/constructs#switch" class="normal-link-page">Biosafety</a>
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</p>
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<p>
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Multigenic assembly of two biosafety devices, comprising the Barnase (male-sterility) with one chromoprotein in each device, AmilCP or AmilGFP (identity preservation). <a href="http://2014.igem.org/Team:Valencia_UPV/Project/results/constructs#biosafety" class="normal-link-page">Results: Constructs-Biosafety</a>
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</p>
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<p>
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Purple plant to preserve its identity expressing ANT1 and JAF13 transcription factors. <a href="http://2014.igem.org/Team:Valencia_UPV/Project/results" class="normal-link-page">Results: Biosafety</a>
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</p>
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<br/><br/>
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<p>
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Diffusion and communication of the project with involved experts and stakeholders.<a href="http://2014.igem.org/Team:Valencia_UPV/policy/activities" class="normal-link-page">Policy and Practices</a>  
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</p>
</p>

Revision as of 19:58, 17 October 2014

Policy and Practices > Achievements


Achievements


As it can bee observed surfing our wiki, at the end of this long road we have accomplished many positive results: