Team:Valencia UPV/Achievements

From 2014.igem.org

(Difference between revisions)
 
(15 intermediate revisions not shown)
Line 16: Line 16:
<ul>
<ul>
-
     <li>Obtained pheromones being among the most abundant plant organic volatile compounds.<span class="red-bold">(Z)-11-hexadecen-1-ol</span> is certainly the most abundant one.</li>
+
     <li>Obtained pheromones being among the most abundant plant organic volatile compounds.<span class="red-bold"> (Z)-11-hexadecen-1-ol</span> is certainly the most abundant one.</li>
-
     <li>Successful and functional assembly of each of the pheromone biosynthetic genes with the plant constitutive promoter P35S. Also multigenic assembly of all three transcription units in a single plasmid. <a href="http://2014.igem.org/Team:Valencia_UPV/Project/results/contructs" class="normal-link-page">Results: Constructions-Biosynthesis</a></li>
+
     <li>Successful and functional assembly of each of the pheromone biosynthetic genes with the plant constitutive promoter P35S. Also multigenic assembly of all three transcription units in a single plasmid. <a href="http://2014.igem.org/Team:Valencia_UPV/Project/results/constructs#biosyn" class="normal-link-page"> Results: Constructs-Biosynthesis</a></li>
-
     <li>Proof of our produced pheromones-insect interaction. <a href="http://2014.igem.org/Team:Valencia_UPV/Project/results/contructs" class="normal-link-page">Results: Electroantennography</a></li>
+
     <li>Proof of our produced pheromones-insect interaction. <a href="http://2014.igem.org/Team:Valencia_UPV/Project/eag" class="normal-link-page">Results: Electroantennography</a></li>
</ul>
</ul>
</li>
</li>
Line 25: Line 25:
<ul class="method">
<ul class="method">
-
<li><a class="black-bold">A broad understanding of plant metabolism, by adapting and exploitation of a genome-scale model of Arabidopsis thaliana primary metabolism able to help us theoretically optimize the pheromone production </a> <a href="http://2014.igem.org/Team:Valencia_UPV/Modeling/fba" class="normal-link-page">Results: Pheromone analysis</a>Modeling: Pheromone Production</a>
+
<li><a class="black-bold">A broad understanding of plant metabolism, by adapting and exploitation of a genome-scale model of Arabidopsis thaliana primary metabolism able to help us theoretically optimize the pheromone production </a> <a href="http://2014.igem.org/Team:Valencia_UPV/Modeling/fba" class="normal-link-page"> Modeling: Pheromone Production</a>
<ul>
<ul>
-
<li>Adapting the AraGEM genome-scale model to our pheromone production pathway by branching the flux of our precursor metabolite Palmitic acic (16:0).</li>
+
<li>Adapting the AraGEM genome-scale model to our pheromone production pathway by branching the flux of our precursor metabolite Palmitic acid (16:0).</li>
<li>Identifying the principal: i) cell compartments and scenarios of the plant metabolism, ii) flux bounds and constraints for each scenario, and iii) the interactions between chemical reactions and substrates related to our pheromone. </li>
<li>Identifying the principal: i) cell compartments and scenarios of the plant metabolism, ii) flux bounds and constraints for each scenario, and iii) the interactions between chemical reactions and substrates related to our pheromone. </li>
<li>Exploring genetic conditions that could improve our pheromone production by Single gene Knock-out analysis. </li>
<li>Exploring genetic conditions that could improve our pheromone production by Single gene Knock-out analysis. </li>
Line 43: Line 43:
<ul>
<ul>
-
     <li>Successful cloning of a trichome-specific promoter (PCPS2) from the genome of N tabacum and subsequent assembly with GFP as a reporter. <a href="http://2014.igem.org/Team:Valencia_UPV/Project/results/constructs#tab2?display=block" class="normal-link-page">Results: Constructs-Pheromone release</a></li>
+
     <li>Successful cloning of a trichome-specific promoter (PCPS2) from the genome of <i>N. tabacum</i> and subsequent assembly with GFP as a reporter. <a href="http://2014.igem.org/Team:Valencia_UPV/Project/results/constructs#phero" class="normal-link-page">Results: Constructs-Pheromone release</a></li>
     <li>Proof of the specificity of this promoter by GFP fluorescence detection.  <a href="http://2014.igem.org/Team:Valencia_UPV/Project/results/trichome_expression" class="normal-link-page">Results: Trichome-specific expression</a></li>
     <li>Proof of the specificity of this promoter by GFP fluorescence detection.  <a href="http://2014.igem.org/Team:Valencia_UPV/Project/results/trichome_expression" class="normal-link-page">Results: Trichome-specific expression</a></li>
-
     <li>Assembly of each gene of the pheromones production pathway with PCPS2 promoter and multigenic assembly with all three transcription units in a single plasmid. <a href="http://2014.igem.org/Team:Valencia_UPV/Project/results/constructs#release" class="normal-link-page">Results: Constructs-Pheromone release</a></a></li>
+
     <li>Assembly of each gene of the pheromones production pathway with PCPS2 promoter and multigenic assembly with all three transcription units in a single plasmid. <a href="http://2014.igem.org/Team:Valencia_UPV/Project/results/constructs#phero" class="normal-link-page">Results: Constructs-Pheromone release</a></a></li>
</ul>
</ul>
</li>
</li>
Line 52: Line 52:
<ul class="method">
<ul class="method">
-
<li><a class="black-bold">A genetic switch able to control gene expression ready to be implemented in the plant. Results: Constructs-Switch</a>
+
<li><a class="black-bold">A Simulation Environment for the pheromone diffusion and moth response behaviour to help us decide where and how much sexy plants we should put in the field. </a> <a href="http://2014.igem.org/Team:Valencia_UPV/Modeling/diffusion" class="normal-link-page"> Modeling: Pheromone Diffusion and Moths Response</a>
 +
<ul>
 +
<li>Implementing an approximation of the diffusion process by the heat diffusion equation and its numerical solution.</li>
 +
<li>Incorporating an approximation of the moth response behavior including female pheromone release into the environment, and male pheromone concentration sensitivity (that allows the male to follow the trace of females).</li>
 +
<li>Adding our sexy plants that produce and release pheromone triggering <i>mating disruption</i>.</li>
 +
<li>Designing a simulation platform available to community that is useful for exploration of parameters and scenarios related with chemical ecology models.</li>
 +
</ul>
 +
</li>
 +
</p>
 +
<br/><br/>
 +
 
 +
 
 +
<ul class="method">
 +
<li><a class="black-bold">A genetic switch able to control gene expression ready to be implemented in the plant.</a>
<ul>
<ul>
     <li>Cloning of the coding sequence from the CUP2 transcription factor from S cerevisiae and assembly with the CaMV constitutive promoter P35S. <a href="http://2014.igem.org/Team:Valencia_UPV/Project/results/constructs#switch" class="normal-link-page">Results: Construct-Switch</a></li>
     <li>Cloning of the coding sequence from the CUP2 transcription factor from S cerevisiae and assembly with the CaMV constitutive promoter P35S. <a href="http://2014.igem.org/Team:Valencia_UPV/Project/results/constructs#switch" class="normal-link-page">Results: Construct-Switch</a></li>
-
     <li>Creation of the Cupper-responsive chimeric promoter and assembly with Firefly luciferase gene as a reporter, P19 as a gene silencing suppressor, and Renilla luciferase as control for Luciferase expression assay.<a href="http://2014.igem.org/Team:Valencia_UPV/Project/results/constructs#switch" class="normal-link-page">Results: Construct-Switch</a></li>
+
     <li>Creation of the Cupper-responsive chimeric promoter and assembly with Firefly luciferase gene as a reporter, P19 as a gene silencing suppressor, and Renilla luciferase as control for Luciferase expression assay. <a href="http://2014.igem.org/Team:Valencia_UPV/Project/results/constructs#switch" class="normal-link-page">Results: Construct-Switch</a></li>
     <li>Multigenic assembly comprising the CUP2 transcriptional unit with the chimeric promoter and reporter gene assembly.  
     <li>Multigenic assembly comprising the CUP2 transcriptional unit with the chimeric promoter and reporter gene assembly.  
<a href="http://2014.igem.org/Team:Valencia_UPV/Project/results/constructs#switch" class="normal-link-page">Results: Construct-Switch</a></li>
<a href="http://2014.igem.org/Team:Valencia_UPV/Project/results/constructs#switch" class="normal-link-page">Results: Construct-Switch</a></li>
Line 64: Line 77:
<ul class="method">
<ul class="method">
-
<li><a class="black-bold">A sterile and dark purple plant safe for living beings and the environment.<a href="http://2014.igem.org/Team:Valencia_UPV/Project/results/constructs#switch" class="normal-link-page">Biosafety</a>
+
<li><a class="black-bold">A sterile and dark purple plant safe for living beings and the environment.<a href="http://2014.igem.org/Team:Valencia_UPV/Project/modules/biosafety" class="normal-link-page">Biosafety</a>
<ul>
<ul>
-
     <li>Multigenic assembly of two biosafety devices, comprising the Barnase (male-sterility) with one chromoprotein in each device, AmilCP or AmilGFP (identity preservation). <a href="http://2014.igem.org/Team:Valencia_UPV/Project/results/constructs#biosafety" class="normal-link-page">Results: Constructs-Biosafety</a></li>
+
     <li>Multigenic assembly of two biosafety devices, comprising the Barnase (male-sterility) with one chromoprotein in each device, AmilCP or AmilGFP (identity preservation). <a href="http://2014.igem.org/Team:Valencia_UPV/Project/results/constructs#biosafe" class="normal-link-page"> Results: Constructs-Biosafety</a></li>
-
     <li>Purple plant to preserve its identity expressing ANT1 and JAF13 transcription factors. <a href="http://2014.igem.org/Team:Valencia_UPV/Project/results" class="normal-link-page">Results: Biosafety</a></li>
+
     <li>Purple plant to preserve its identity expressing SlANT1 and SlJAF13 transcription factors. <a href="http://2014.igem.org/Team:Valencia_UPV/Project/results/biosafety" class="normal-link-page"> Results: Biosafety</a></li>
</ul>
</ul>
Line 75: Line 88:
<p>
<p>
-
<a class="black-bold">Diffusion and communication of the project with involved experts and stakeholders.<a href="http://2014.igem.org/Team:Valencia_UPV/policy/activities" class="normal-link-page">Policy and Practices</a></a>  
+
<a class="black-bold">Diffusion and communication of the project with involved experts and stakeholders.<a href="http://2014.igem.org/Team:Valencia_UPV/policy/overview" class="normal-link-page"> Policy and Practices</a></a>  
</p>
</p>

Latest revision as of 03:52, 18 October 2014

Policy and Practices > Achievements


Achievements


As it can bee observed surfing our wiki, at the end of this long road we have accomplished many positive results: