Team:Valencia UPV/Achievements

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<p><h3 class="hook" align="left"><a>Policy and Practices</a> > <a>Activities</a></h3></p><br/>
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<p><h3 class="hook" align="left"><a>Policy and Practices</a> > <a>Achievements</a></h3></p><br/>
<div align="center"><span class="coda"><roja>A</roja>chievements</span> </div><br/><br/>
<div align="center"><span class="coda"><roja>A</roja>chievements</span> </div><br/><br/>
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<p>As it can bee observed surfing our wiki, at the end of this long road we have accomplished many positive results:</p>
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<ul class="method">
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<li><a class="black-bold">A plant able to produce three insect sexual pheromones from moths to produce insects mating disruption, the Sexy Plant.:</a> <a href="http://2014.igem.org/Team:Valencia_UPV/Project/results/pheromone_analysis" class="normal-link-page">Results: Pheromone analysis</a>
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    <li>Obtained pheromones being among the most abundant plant organic volatile compounds.<span class="red-bold"> (Z)-11-hexadecen-1-ol</span> is certainly the most abundant one.</li>
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    <li>Successful and functional assembly of each of the pheromone biosynthetic genes with the plant constitutive promoter P35S. Also multigenic assembly of all three transcription units in a single plasmid. <a href="http://2014.igem.org/Team:Valencia_UPV/Project/results/constructs#biosyn" class="normal-link-page"> Results: Constructs-Biosynthesis</a></li>
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    <li>Proof of our produced pheromones-insect interaction. <a href="http://2014.igem.org/Team:Valencia_UPV/Project/eag" class="normal-link-page">Results: Electroantennography</a></li>
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</ul>
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</li>
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<ul class="method">
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<li><a class="black-bold">A broad understanding of plant metabolism, by adapting and exploitation of a genome-scale model of Arabidopsis thaliana primary metabolism able to help us theoretically optimize the pheromone production </a> <a href="http://2014.igem.org/Team:Valencia_UPV/Modeling/fba" class="normal-link-page"> Modeling: Pheromone Production</a>
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<li>Adapting the AraGEM genome-scale model to our pheromone production pathway by branching the flux of our precursor metabolite Palmitic acid (16:0).</li>
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<li>Identifying the principal: i) cell compartments and scenarios of the plant metabolism, ii) flux bounds and constraints for each scenario, and iii) the interactions between chemical reactions and substrates related to our pheromone. </li>
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<li>Exploring genetic conditions that could improve our pheromone production by Single gene Knock-out analysis. </li>
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<li>Obtaining optimal condition  for the coupled yield of pheromone and biomass production as a function of photons consumption.</li>
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</ul>
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</li>
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</p><br/><br/>
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<ul class="method">
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<li><a class="black-bold">A plant potentially able to release the produced pheromones into the environment.</a>
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<ul>
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    <li>Successful cloning of a trichome-specific promoter (PCPS2) from the genome of <i>N. tabacum</i> and subsequent assembly with GFP as a reporter. <a href="http://2014.igem.org/Team:Valencia_UPV/Project/results/constructs#phero" class="normal-link-page">Results: Constructs-Pheromone release</a></li>
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    <li>Proof of the specificity of this promoter by GFP fluorescence detection.  <a href="http://2014.igem.org/Team:Valencia_UPV/Project/results/trichome_expression" class="normal-link-page">Results: Trichome-specific expression</a></li>
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    <li>Assembly of each gene of the pheromones production pathway with PCPS2 promoter and multigenic assembly with all three transcription units in a single plasmid. <a href="http://2014.igem.org/Team:Valencia_UPV/Project/results/constructs#phero" class="normal-link-page">Results: Constructs-Pheromone release</a></a></li>
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</ul>
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</li>
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</p><br/><br/>
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<ul class="method">
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<li><a class="black-bold">A Simulation Environment for the pheromone diffusion and moth response behaviour to help us decide where and how much sexy plants we should put in the field. </a> <a href="http://2014.igem.org/Team:Valencia_UPV/Modeling/diffusion" class="normal-link-page"> Modeling: Pheromone Diffusion and Moths Response</a>
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<ul>
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<li>Implementing an approximation of the diffusion process by the heat diffusion equation and its numerical solution.</li>
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<li>Incorporating an approximation of the moth response behavior including female pheromone release into the environment, and male pheromone concentration sensitivity (that allows the male to follow the trace of females).</li>
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<li>Adding our sexy plants that produce and release pheromone triggering <i>mating disruption</i>.</li>
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<li>Designing a simulation platform available to community that is useful for exploration of parameters and scenarios related with chemical ecology models.</li>
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</ul>
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</li>
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</p>
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<br/><br/>
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<ul class="method">
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<li><a class="black-bold">A genetic switch able to control gene expression ready to be implemented in the plant.</a>
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<ul>
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    <li>Cloning of the coding sequence from the CUP2 transcription factor from S cerevisiae and assembly with the CaMV constitutive promoter P35S. <a href="http://2014.igem.org/Team:Valencia_UPV/Project/results/constructs#switch" class="normal-link-page">Results: Construct-Switch</a></li>
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    <li>Creation of the Cupper-responsive chimeric promoter and assembly with Firefly luciferase gene as a reporter, P19 as a gene silencing suppressor, and Renilla luciferase as control for Luciferase expression assay. <a href="http://2014.igem.org/Team:Valencia_UPV/Project/results/constructs#switch" class="normal-link-page">Results: Construct-Switch</a></li>
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    <li>Multigenic assembly comprising the CUP2 transcriptional unit with the chimeric promoter and reporter gene assembly.
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<a href="http://2014.igem.org/Team:Valencia_UPV/Project/results/constructs#switch" class="normal-link-page">Results: Construct-Switch</a></li>
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</ul>
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</li>
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</p><br/><br/>
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<ul class="method">
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<li><a class="black-bold">A sterile and dark purple plant safe for living beings and the environment.<a href="http://2014.igem.org/Team:Valencia_UPV/Project/modules/biosafety" class="normal-link-page">Biosafety</a>
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<ul>
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    <li>Multigenic assembly of two biosafety devices, comprising the Barnase (male-sterility) with one chromoprotein in each device, AmilCP or AmilGFP (identity preservation). <a href="http://2014.igem.org/Team:Valencia_UPV/Project/results/constructs#biosafe" class="normal-link-page"> Results: Constructs-Biosafety</a></li>
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    <li>Purple plant to preserve its identity expressing SlANT1 and SlJAF13 transcription factors. <a href="http://2014.igem.org/Team:Valencia_UPV/Project/results/biosafety" class="normal-link-page"> Results: Biosafety</a></li>
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</ul>
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</li>
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</p><br/><br/>
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<p>
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<a class="black-bold">Diffusion and communication of the project with involved experts and stakeholders.<a href="http://2014.igem.org/Team:Valencia_UPV/policy/overview" class="normal-link-page"> Policy and Practices</a></a>
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</p>
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Latest revision as of 03:52, 18 October 2014

Policy and Practices > Achievements


Achievements


As it can bee observed surfing our wiki, at the end of this long road we have accomplished many positive results: