Team:UT-Tokyo/CTCD/Content

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<li><a href="#">~~Contents1~~</a></li>

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<li><a href="Javascript:loadContent('Project-block','Project-1')">Introduction</a></li>

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<li><a href="#">~~Contents2~~</a></li>

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<li><a href="Javascript:loadContent('Project-block','Project-2')">System</a></li>

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<li><a href="Javascript:loadContent('Project-block','Project-3')">Application</a></li>

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<li><a href="#">~~Contents1</a></li>~~

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<li><a href="Javascript:loadContent('Result-block','Result-1')">miRNA-binding sites</a></li>

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<li><a href="Javascript:loadContent('Lab-block','Lab-2')">Protocol</a></li>

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<li><a href="#">~~Contents1</a></li>~~

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~~<li><a href="#">Contents2~~</a></li>

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<li><a href="Javascript:loadContent('Achievement-block','Achievement-1')">Judging Form</a></li>

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~~<li><a href="#">Contents2~~</a></li>

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~~<ul class="subContList">~~

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<li><a href="Javascript:loadContent('Attribution-block','Attribution-1')">Attribution</a></li>

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<li><a href="Javascript:loadContent('Attribution-block','Attribution-2')">Acknowledgement</a></li>

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<li><a href="Javascript:loadContent('Attribution-block','Attribution-3')">Sponsors</a></li>

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<li><a href="Javascript:loadContent('Humanpractice-block','Humanpractice-1')">Human Practice</a></li>

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<li><a href="Javascript:loadContent('Humanpractice-block','Humanpractice-2')">Ethics</a></li>

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~~<img src~~ = "~~https~~:~~//static.igem.org/mediawiki/2014/d/db/Sub_background.png~~" ~~class = "contTitle"/~~>

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<p>The word, "counter", will remind you the machine with which you can count the number of the objects, such as people and cars. Some people familiar with electronic circuits may remind the logic circuit. In any case, both are the memory device of the order of inputs and are important for our lives.<br /><br />Cellular counters also memorize the number of events. In nature, there are tellomere length regulation, cell aggregation and so on. <br />Ari et al constructed the cellular counter with synthetic biological approach, termed the riboregulated transcriptional cascade (RTC) counter[1].The state transition occurs after arabinose induction (Fig. 1). The system is regulated by riboregulators. The Biobrick part of this cellular counter has already existed, which was constructed by Tokyo-Nokogen 2009 and was named BBa_K225002, BBa_K25003 [2]. <br />In order to expand the function of this counter, we added reset system. The reset sysytem realizes the movement from any state to the initial state after the particular input and is expected to movement from down stream states to upstream states by respective inputs, which we regard as automaton. Our counter utilizes the regulation system of sigma factor and anti-σ factor as the key of its resetting mechanism. In addition to reset function, the number of states can be increased more easily, because what we have to think is only the combination of σ and anti-σ factor, that is, we do not have to find new polymerase. Moreover, because there is orthonal relation between sigma and anti-sigma factor, we can choose combinations which help us avoid crosstalk problem. <br /><br />Fig. 1 The concept of RTC counter. After first, second and third induction of arabinose, the state of cells moves from 0 to 1, 1 to 2 and 2 to 3, respectively.<br /><br />[1]Ari E. Friedland, et al., Science 324, 1199 (2009). <br />[2]https://2009.igem.org/Team:Tokyo-Nokogen/Parts</p>

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~~<img src~~ = "~~https~~:~~//static.igem.org/mediawiki/2014/e/e4/Sub_memory.png~~" ~~class = "contTitle" /~~>

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~~<h3>σ factor~~</h3>

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<p>σ factor is a subunit of RNA polymerase related to promoter recognitions. There are two types of sigma factors, one that is housekeeping (expressed constantly) and another that is expressed under some conditions. It is possible to deliver genes of sigma factors that are not derived from some species to another species, so we can use many kinds of σ factor. Which promoter RNA polymerase tends to bind to is decided by which σ factor RNA polymerase is bound. Especially some σ factors promote only transcription of a specific promoters. Therefore if we choose a set of sigma factors and promoters skillfully, we can control transcription without crosstalk. Here, “without crosstalk” means every σ factor in the set promotes only transcription of the cognate promoter.<br />Anti-~~σ is also a protein which is related to transcriptional control by σ factors. Anti~~-~~σ prevents σ factors from binding to RNA polymerase. Similarly as σ factor, there are many kinds of anti~~-~~σ and we can choose a set of σ factors, promoters, and anti~~-~~σs which has no crosstalk. In our project, we use such a set of σ factors, promoters, and anti-σs.</p~~>

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<~~h3>σ~~-~~memory construction</h3~~>

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<p>This is the construction of our σ-memory. If input A exists, σ factor is expressed. Then the positive feedback circuits of σ factor starts producing σ factor, and consequently σ factor will remain. Though if input B exists, anti-σ is expressed and the positive feedback circuits is inhibited. Both σ factor and anti-σ are subjects to degradation, so all of them are decomposed after some time and σ-memory returns to original state.<br />We can regard existence/absence of σ factor as 1/0 of memory, and this values of memory can switch by input A or input B. Using the promoter which is cognate to the σ factor, the information whether the value of memory is 1 or 0 can be derived. For example, consider the circuits on the right. The repotor is expressed if and only if σ-memory's value is 1 (i.e. σ factor exists).<~~br />In addition, no crosstalk sets of σ factors, promoters, and anti~~-~~σs enable us to make multi~~-σ-~~memory gene circuits. To make the explanation easier, consider the case in which E. coli has two σ memories, σA~~-memory and σB-memory. The value of σA-memory change from 0 to 1 if input A1 exists and change from 1 to 0 if inputs B1 exists. Also the value of σB-memory change from 0 to 1 if input A2 exists and change from 1 to 0 if input B2 exists. If the four input A1, B1, A2, and B2 have no crosstalk, σA-memory and σB-memory also have no crosstalk. For example, when input A1 exists, only the value of σA-memory change from 0 to 1 since σA promotes only transcription from PσA (promoter that is cognate to σA), and has no effect on σB. The same is true of input B1 and anti-σA (anti-σ that is cognate to σA). So it can be confidently said that E. coli has two σ memories.<br />Since this construction has a positive feedback, leakage of promoter may be a serious problem. If the promoter has leakage and σ factor is expressed when input A dose not exist, this error is enlarged by positive feedback. However if the leakage of anti-σ is satisfactory large, σ factor dose not produced from positive feedback when leak of σ factor occurs. Therefore leakage is not an obstacle of our project if we choose the promoter skillfully.

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<~~h3>σ~~-~~memory and automaton</h3>~~

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<p>Speaking easily, an automaton is referred to what has states and transition rules. Every state has a transition rule, say it is decided what is the next state if an input exists. For example, the automaton on the right has five states, 0, 1, 2, 3, 4, and transition rules are represented by arrows. If the current state is 0 and the input is b, the next state is 2. Transition rules having loops or feedbacks are allowed.<br />σ-memory can be considered an automaton. Every tuple of σ-memories' value (say, (1, 1, 0, 1, 0)) corresponds to a state. It is possible to use one σ-memory as an inputs of another σ-memory. For example, consider the gene circuit on the right.The value of σA-memory( a σ-memory which uses σA in the construction as a σ factor) change from 0 to 1 if arabinose exists, also the value of σB-memory change from 0 to 1 if the value of σA-memory is 1 and IPTG exists. (This condition can be written in terms of mathematical logic, namely AND(σA, IPTG)) These gene circuits can be considered as the automaton on the right. Gene circuits which work as logic circuits (says AND, OR, NAND, etc...) have been designed.</p>

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~~<img src~~ = "~~https~~:~~//static.igem.org/mediawiki/2014/9/93/Sub_appli.png~~" ~~class = "contTitle" /~~>

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~~<h3>resettable counter by σ-memory~~</h3>

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<~~p>Since a counter is one kind of automata, σ~~-memory can be applied for making resettable counters. It can count the number of induction events of arabinose, and express a reporter correspond to each states. In addition, the count can be reset by IPTG induction.<br />2-~~counter is a counter that can count up to 2, and is an automaton that has three states, 0, 1, and 2. This automaton can be constructed by σ~~-~~memory as following. Two kinds of σ factor are necessary for constructing the 2~~-counter, so for the convenience we will call these two kinds of σ factors σA and σB. In state 0, both σA and σB do not exist (i.e. σA-memory and σB-memory is 0). In state 1, only σA exists and in states 2, both σ factors exist. The input is the induction of arabinose or IPTG, and the transition rules with it are showed in the Figure.<br />A gene circuit that realizes this automaton can be represented as following. The value of σA changes from 0 to 1 after arabinose induction (i.e. σA is expressed when arabinose exists). This change corresponds to the transition from state from 0 to 1.The value of σB-memory changes from 0 to 1 when both arabinose and σA exist. 【説明不足】Therefore the AND function is necessary. This change corresponds to the transition from state 1 to state 2. Both the value of σA-memory and σB-memory change from 1 to 0 after IPTG induction (i.e. anti-σA and σB is expressed when IPTG exist).</p>

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<~~h3>riboregulator</h3>~~

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~~<p>According to the discussion in 2~~-1, it is necessary to use AND function for the construction of a resettable counter. Therefore a riboregulator is used for the realization of the AND function.<br />A riboregulator is a post-transcriptional regulation system composed of two kinds of RNAs. Cis- repressed mRNA(crRNA) forms a stem-loop and its ribosomal binding site (RBS) is covered. Consequently the gene coded in cis-repressed mRNA isn't translated. However if trans-activating RNA (taRNA) exist, crRNA and taRNA are hybridized and RBS gets exposed and translation starts.<br />Namely, the gene coded in crRNA is expressed only when both crRNA and taRNA is transcripted. Therefore this system can be considered as the AND function. For example, consider the gene circuit, Plac-taRNA-d.term-Pbad-cr-RBS-GFP-d.term.(cr is the sequence in crRNA which binds to RBS) If and only if both IPTG and arabinose exists, GFP is expressed. Therefore, the riboregulator can combine two promoters and produce AND functions</p>

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~~<h3>resettable counter construction~~</h3>

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<p>The construction of our resettable counter is explained here.<br /><br />σ20 and σ11 are used as σA and σB as mentioned above respectively. The promoters correspond to σ20 and σ11 are Pσ20 and Pσ11, and the anti-~~σs are anti~~-~~20 and anti~~-~~11 respectively. GFP is used as a reporter. <br />At first, both the value of σ20~~-memory and σ11-memory are 0. Only the crRNA coding σ20, which is at downstream of constitutive promoter is translated. After the first induction of arabinose, taRNA at the downstream of PBAD is transcribed and the crRNA coding σ20 is translated, and the value of σ20-memory changes from 0 to 1. Since σ20 exists, crRNA coding σ11 at the downstream of Pσ20 is transcribed. After the second induction of arabinose, taRNA is transcribed and σ11 is expressed, and the value of σ11-memory changes from 0 to 1. At this time, GFP at the downstream of Pσ11 is expressed and we can check the count as 2.<br />After an induction of IPTG, anti-σ20 and σ11 are expressed, and the value of σ20-memory and σ11-memory will be 0. Therefore the count is reset.<br /><br />Since there are many kinds of pair of σ factors and cognate promoters which have no crosstalk, n-counter can be made by the same way. Besides, the construction of 2-counter can be simplified.<br />This simplified counter is made by only one σ factor. It works as the same way as original 2-counter unill 1 count. After 1 count, crRNA coding GFP is transcribed at the downstream of Pσ. Therefore when the next arabinose induction occurs, GFP is translated. This expression can be considered a report of 2 count. This simplified counter can be also extended to n count. Simplified counter can count up to larger numbers compared to the original counter even when the same number of σ factors are used, but cannot be reset from their final count. We did experiments on this simplified counter.</p>

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~~<!-- Project application -~~->

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<p class = "name">~~MASAKI ONO~~</p>

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<p class = "name">YOICHI IRIE</p>

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<dd>~~Masaki Ono~~</dd>

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<dd>Yoichi Irie</dd>

<dt>Belong to</dt>

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<dd>The University of Tokyo, ~~Literature, Sophmore~~</dd>

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<dd>The University of Tokyo, Arts and Sciences</dd>

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<dd>~~Modeling~~</dd>

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<dd>Team leader</dd>

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<p class ="comment">~~I want~~ to ~~be a doctor.~~</p>

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<p class ="comment">My dream is to make me robuster against stress caused by iGEM in synthetic biology!</p>

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<p class = "name">~~MASAKI ONO~~</p>

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<p class = "name" style="font-size:35px;">CRISTIAN DAVID PENA MARINEZ</p>

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<dd>~~Masaki Ono~~</dd>

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<dd>Cristian David Pena Martinez</dd>

<dt>Belong to</dt>

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<dd>~~The University~~ of ~~Tokyo~~, ~~Literature~~, ~~Sophmore~~</dd>

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<dd>Department of Regulatory Biology, Faculty of Science, Saitama University</dd>

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<dd>~~Modeling~~</dd>

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<dd>Design</dd>

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<p class ="comment">~~I want to be a doctor.~~</p>

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<p class ="comment">"The only concrete proof of the human existence is: poetry" - Luis Cardoza y Aragon</p>

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<dd>Masaki Ono</dd>

<dt>Belong to</dt>

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<dd>The University of Tokyo, ~~Literature, Sophmore</dd>~~

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<dd>The University of Tokyo, Arts and Sciences, Sophmore</dd>

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~~<dt>Job</dt>~~

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~~<dd>Modeling</dd>~~

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~~</dl>~~

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~~<p class ="comment">I want to be a doctor.</p>~~

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~~<p>iGEM UT-Tokyo</p>~~

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~~<img src = "https://static.igem.org/mediawiki/2014/9/98/Ui_close_40.png" class = "closebutton" />~~

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~~<div class = "member">~~

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~~<p class = "name">MASAKI ONO</p>~~

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~~<dl class = "profile">~~

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~~<dt>Name</dt>~~

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~~<dd>Masaki Ono</dd>~~

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~~<dt>Belong to</dt>~~

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~~<dd>The University of Tokyo, Literature~~, Sophmore</dd>

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<dd>Modeling</dd>

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<p class = "name">~~MASAKI ONO~~</p>

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<p class = "name">YUMENO KOGA</p>

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<dd>~~Masaki Ono~~</dd>

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<dd>Yumeno Koga</dd>

<dt>Belong to</dt>

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<dd>~~The University~~ of ~~Tokyo~~, ~~Literature~~, ~~Sophmore~~</dd>

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<dd>Department of Lifescience & Medical Bioscience, School of Advanced Science and Engineering, Waseda University</dd>

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<dd>~~Modeling~~</dd>

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<dd>Presenter</dd>

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<p class ="comment">I ~~want to be~~ a ~~doctor~~.</p>

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<p class ="comment">I'm a "wasejo".</p>

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<p class = "name">~~MASAKI ONO~~</p>

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<p class = "name">KENTO NAKAMURA</p>

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<dd>~~Masaki Ono~~</dd>

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<dd>Kento Nakamura</dd>

<dt>Belong to</dt>

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<dd>The University of Tokyo~~, Literature, Sophmore~~</dd>

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<dd>College of Arts and Sciences, The University of Tokyo</dd>

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<dd>~~Modeling~~</dd>

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<dd>Experimenter, Presenter</dd>

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<p class ="comment">I ~~want to be a doctor~~.</p>

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<p class ="comment">"I think, therefore I am" by R. Descartes<br />"I multiply, therefore I am" by E.coli <br />"I hope so..." by Experimenter</p>

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<p class = "name">~~MASAKI ONO~~</p>

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<p class = "name">SOH NAKASHIMA</p>

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<dd>~~Masaki Ono~~</dd>

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<dd>Soh Nakashima</dd>

<dt>Belong to</dt>

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<dd>The University of Tokyo~~, Literature, Sophmore~~</dd>

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<dd>College of Arts and Sciences, The University of Tokyo</dd>

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<dd>~~Modeling~~</dd>

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<dd>Experimenter</dd>

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<p class ="comment">~~I want to be~~ a ~~doctor~~.</p>

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<p class ="comment">follow me on Twitter(<a href="https://twitter.com/dolicas_" target="_blank" >@dolicas_</a>)</p>

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<p class = "name">~~MASAKI ONO~~</p>

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<p class = "name">MANABU NISHIURA</p>

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<dd>~~Masaki Ono~~</dd>

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<dd>Manabu Nishiura</dd>

<dt>Belong to</dt>

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<dd>The University of Tokyo, ~~Literature, Sophmore~~</dd>

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<dd>The University of Tokyo, Arts and Sciences</dd>

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<dd>Modeling</dd>

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<dd>Modeling,Presenter</dd>

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<p class ="comment">I ~~want to be~~ a ~~doctor~~.</p>

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<p class ="comment"> I am a Feynman Diagram.</p>

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<p class = "name">~~MASAKI ONO~~</p>

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<p class = "name">YOSHIKI OKESAKU</p>

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<dd>~~Masaki Ono~~</dd>

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<dd>Yoshiki Okesaku</dd>

<dt>Belong to</dt>

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<dd>The University of Tokyo~~, Literature, Sophmore~~</dd>

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<dd> Department of Physics, Graduate School of Science, The University of Tokyo</dd>

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<dd>~~Modeling~~</dd>

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<dd>Design(DOKATA)</dd>

</dl>

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<p class ="comment">I ~~want to be a doctor~~.</p>

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<p class ="comment">I am majoring in the MDS theory. The MDS theory can describe how our universe had begun and will end.</p>

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<p class = "name">~~MASAKI ONO~~</p>

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<p class = "name">HAJIME TAKEMURA</p>

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<dd>~~Masaki Ono~~</dd>

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<dd>Hajime Takemura</dd>

<dt>Belong to</dt>

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<dd>The University of Tokyo, ~~Literature~~, ~~Sophmore~~</dd>

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<dd>The University of Tokyo, Junior,Faculty of Pharmaceutical Science</dd>

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<dd>~~Modeling~~</dd>

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<dd>Experiment</dd>

</dl>

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<p class ="comment">I want to be a ~~doctor~~.</p>

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<p class ="comment">I want to make a new medicine.</p>

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<p class = "name">~~MASAKI ONO~~</p>

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<p class = "name">KENTARO TARA</p>

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<dd>~~Masaki Ono~~</dd>

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<dd>Kentaro Tara</dd>

<dt>Belong to</dt>

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<dd>The University of Tokyo~~, Literature, Sophmore~~</dd>

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<dd>Arts and Sciences, The University of Tokyo</dd>

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<dd>Modeling</dd>

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<dd>Experiment, Modeling</dd>

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<p class ="comment">~~I want to be a doctor~~.</p>

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<p class ="comment">Maps relax me.</p>

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<p class = "name">~~MASAKI ONO~~</p>

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<p class = "name">YUEWEN DING</p>

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<dd>~~Masaki Ono~~</dd>

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<dd>Yuewen Ding</dd>

<dt>Belong to</dt>

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<dd>The ~~University~~ of Tokyo~~, Literature, Sophmore~~</dd>

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<dd>Faculty of Pharmaceutical Science, The university of Tokyo</dd>

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<dd>~~Modeling~~</dd>

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<dd>Public relation</dd>

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<p class ="comment">~~I want to be a doctor~~.</p>

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<p class ="comment">Eating is sweeter than Life.</p>

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<p class = "name">~~MASAKI ONO~~</p>

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<p class = "name">YOSHIHIKO TOMOFUJI</p>

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<dd>~~Masaki Ono~~</dd>

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<dd>Yoshihiko Tomofuji</dd>

<dt>Belong to</dt>

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<dd>The ~~University~~ of Tokyo, ~~Literature, Sophmore~~</dd>

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<dd>The university of Tokyo, faculty of medicine</dd>

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<dd>~~Modeling~~</dd>

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</dl>

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<p class ="comment">I ~~want to be~~ a ~~doctor~~.</p>

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</div>

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<p class = "name">~~MASAKI ONO~~</p>

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<p class = "name">KOHTA TOSHIMITSU</p>

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<dd>~~Masaki Ono~~</dd>

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<dd>Kohta Toshimitsu</dd>

<dt>Belong to</dt>

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<dd>~~The University~~ of ~~Tokyo~~, ~~Literature, Sophmore~~</dd>

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<dd>Faculty of Pharmacy,　Keio University</dd>

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<dd>~~Modeling~~</dd>

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<dd>Adviser</dd>

</dl>

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<p class ="comment">I ~~want to be a doctor~~.</p>

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<p class ="comment">I'm so sick of pipetting.</p>

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<p class = "name">~~MASAKI ONO~~</p>

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<p class = "name">KEISUKE TSUKADA</p>

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<dd>~~Masaki Ono~~</dd>

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<dd>Keisuke Tsukada</dd>

<dt>Belong to</dt>

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<dd>The University of Tokyo~~, Literature, Sophmore~~</dd>

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<dd>Department of Biophysics and Biochemistry , Faculty of Science, The University of Tokyo</dd>

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<dd>Modeling</dd>

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<dd>Experiment, Modeling</dd>

</dl>

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<p class ="comment">~~I want to be a doctor.~~</p>

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<p class ="comment">itininnmaenokenkyuusyaninaretaraiina</p>

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<p class = "name">~~MASAKI ONO~~</p>

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<p class = "name">YUTO YAMANAKA</p>

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<dd>~~Masaki Ono~~</dd>

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<dd>Yuto Yamanaka</dd>

<dt>Belong to</dt>

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<dd>~~The University~~ of ~~Tokyo~~, ~~Literature~~, ~~Sophmore~~</dd>

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<dd>Department of Electrical Engineering and Bioscience, School of Advanced Science and Engineering, Waseda University</dd>

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<dd>~~Modeling~~</dd>

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<dd>Experimenter, Presenter</dd>

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</dl>

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<p class ="comment">I like Falcon tubes.</p>

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</div>

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+

<p>iGEM UT-Tokyo</p>

+

+

</div>

+

+

<p class = "name">SHUNSUKE SUMI</p>

+

+

+

+

<dd>Shunsuke Sumi</dd>

+

<dt>Belong to</dt>

+

<dd>Department of medicine, Faculty of medicine, The Jikei University School of Medicine</dd>

+

+

+

</dl>

+

<p class ="comment">a tree has no emotion.</p>

+

</div>

+

</div>

+

</div>

+

+

+

+

</div>

+

+

+

<p>iGEM UT-Tokyo</p>

+

+

</div>

+

+

<p class = "name">NARUKI YOSHIKAWA</p>

+

+

+

+

<dd>Naruki Yoshikawa</dd>

+

<dt>Belong to</dt>

+

<dd>College of Arts and Sciences, The University of Tokyo</dd>

+

+

<dd>Experimenter</dd>

+

</dl>

+

<p class ="comment">Three billion devices run Java, but my devices don't.</p>

+

</div>

+

</div>

+

</div>

+

+

+

+

</div>

+

+

+

<p>iGEM UT-Tokyo</p>

+

+

</div>

+

+

<p class = "name">NOBUHIRO HIURA</p>

+

+

+

+

<dd>Nobuhiro Hiura</dd>

+

<dt>Belong to</dt>

+

<dd>Department of Regulatory Biology, Faculty of Science, Saitama University</dd>

+

+

<dd>Experimenter</dd>

+

</dl>

+

<p class ="comment">Let's go to Saitama, Japan!</p>

+

</div>

+

</div>

+

</div>

+

+

+

+

</div>

+

+

+

<p>iGEM UT-Tokyo</p>

+

+

</div>

+

+

<p class = "name">HIROKI TSUBOI</p>

+

+

+

+

<dd>Hiroki Tsuboi</dd>

+

<dt>Belong to</dt>

+

<dd>Waseda University, School of Fundamental Science and Engineering, Computer Science and Engineering</dd>

+

+

+

</dl>

+

<p class ="comment">After wiki freeze, I will make other Web Site</p>

+

</div>

+

</div>

+

</div>

+

+

+

+

</div>

+

+

+

<p>iGEM UT-Tokyo</p>

+

+

</div>

+

+

<p class = "name">SHIGETAKA KOBARI</p>

+

+

+

+

<dd>Shigetaka Kobari</dd>

+

<dt>Belong to</dt>

+

<dd>The university of Tokyo, faculty of medicine</dd>

+

+

+

</dl>

+

<p class ="comment">a tree has no emotion.</p>

+

</div>

+

</div>

+

</div>

+

+

+

+

</div>

+

+

+

<p>iGEM UT-Tokyo</p>

+

+

</div>

+

+

<p class = "name">MASATO ISHIKAWA</p>

+

+

+

+

<dd>Masato Ishikawa</dd>

+

<dt>Belong to</dt>

+

<dd>College of Arts and Sciences, The University of Tokyo</dd>

+

+

<dd>Presenter</dd>

+

</dl>

+

<p class ="comment">I want to study pharmacy.</p>

+

</div>

+

</div>

+

</div>

+

+

+

+

</div>

+

+

+

<p>iGEM UT-Tokyo</p>

+

+

</div>

+

+

<p class = "name">MASAYUKI OSAWA</p>

+

+

+

+

<dd>Masayuki Osawa</dd>

+

<dt>Belong to</dt>

+

<dd>Department of medicine, Faculty of medicine, The Jikei University School of Medicine</dd>

+

+

<dd>Woodkeeper</dd>

+

</dl>

+

<p class ="comment">I take care of crazy tree.</p>

+

</div>

+

</div>

+

</div>

+

+

+

+

</div>

+

+

+

<p>iGEM UT-Tokyo</p>

+

+

</div>

+

+

<p class = "name">TAKEHUMI YOSHIKAWA</p>

+

+

+

+

<dd>Takehumi Yoshikawa</dd>

+

<dt>Belong to</dt>

+

<dd>Department of Bioinformatics and Systems Biology, Faculty of Science, The University of Tokyo</dd>

+

+

<dd>Experiment Leader</dd>

+

</dl>

+

+

</div>

+

</div>

+

</div>

+

+

+

+

</div>

+

+

+

<p>iGEM UT-Tokyo</p>

+

+

</div>

+

+

<p class = "name">ATSUKI ITO</p>

+

+

+

+

<dd>Atsuki Ito</dd>

+

<dt>Belong to</dt>

+

<dd>Department of Bioscience, Faculty of Engineering,Tokyo University of Science</dd>

+

+

<dd>Experimenter</dd>

+

</dl>

+

<p class ="comment">kimuwaipu,azinai...</p>

+

</div>

+

</div>

+

</div>

+

+

+

+

</div>

+

+

+

<p>iGEM UT-Tokyo</p>

+

+

</div>

+

+

<p class = "name">PROF.WAKAMOTO</p>

+

+

+

+

<dd>Yuichi Wakamoto</dd>

+

<dt>Belong to</dt>

+

<dd>Center for Complex Systems Biology The University of Tokyo, Dapartment of Basic Science, Graduate School of Arts and Sciences, The University of Tokyo</dd>

+

+

<dd>Instructor</dd>

</dl>

-

~~<p class ="comment">I want to be a doctor.</p>~~

</div>

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</div>

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</div>

@@ Line 8: / Line 8: @@
 <li id="Project"><a href="Javascript:open('Project')"><img src="https://static.igem.org/mediawiki/2014/4/42/LinkCTC_projecton.png" class="contList" alt="https://static.igem.org/mediawiki/2014/7/73/LinkCTC_projectopen.png"></a>
 <ul class="subContList">
-<li><a href="#">Contents1</a></li>
+<li><a href="Javascript:loadContent('Project-block','Project-1')">Introduction</a></li>
-<li><a href="#">Contents2</a></li>
+<li><a href="Javascript:loadContent('Project-block','Project-2')">System</a></li>
+<li><a href="Javascript:loadContent('Project-block','Project-3')">Application</a></li>
 </ul>
 </li>
 <li id="Result"><a href="Javascript:open('Result')"><img src="https://static.igem.org/mediawiki/2014/1/11/LinkCTC_resulton.png" class="contList" alt="https://static.igem.org/mediawiki/2014/8/83/LinkCTC_resultopen.png"></a>
 <ul class="subContList">
-<li><a href="#">Contents1</a></li>
+<li><a href="Javascript:loadContent('Result-block','Result-1')">miRNA-binding sites</a></li>
-<li><a href="#">Contents2</a></li>
 </ul>
 </li>
 <li id="Lab"><a href="Javascript:open('Lab')"><img src="https://static.igem.org/mediawiki/2014/6/6d/LinkCTC_labon.png" class="contList" alt="https://static.igem.org/mediawiki/2014/d/de/LinkCTC_labopen.png"></a>
 <ul class="subContList">
-<li><a href="#">Contents1</a></li>
+<li><a href="Javascript:loadContent('Lab-block','Lab-1')">Parts</a></li>
-<li><a href="#">Contents2</a></li>
+<li><a href="Javascript:loadContent('Lab-block','Lab-2')">Protocol</a></li>
+<li><a href="Javascript:loadContent('Lab-block','Lab-3')">Lab Note</a></li>
 </ul>
 </li>
 <li id="Modeling"><a href="Javascript:open('Modeling')"><img src="https://static.igem.org/mediawiki/2014/4/42/LinkCTC_modelingon.png" class="contList" alt="https://static.igem.org/mediawiki/2014/5/56/LinkCTC_modelingopen.png"></a>
 <ul class="subContList">
-<li><a href="#">Contents1</a></li>
+<li><a href="Javascript:loadContent('Modeling-block','Modeling-1')">BBa_K747096</a></li>
-<li><a href="#">Contents2</a></li>
 </ul>
 </li>
-<li id="Achieve"><a href="Javascript:open('Achieve')"><img src="https://static.igem.org/mediawiki/2014/8/88/LinkCTC_achieon.png" class="contList" alt="https://static.igem.org/mediawiki/2014/4/41/LinkCTC_achieopen.png"></a>
+<li id="Achievement"><a href="Javascript:open('Achievement')"><img src="https://static.igem.org/mediawiki/2014/8/88/LinkCTC_achieon.png" class="contList" alt="https://static.igem.org/mediawiki/2014/4/41/LinkCTC_achieopen.png"></a>
 <ul class="subContList">
-<li><a href="#">Contents1</a></li>
+<li><a href="Javascript:loadContent('Achievement-block','Achievement-1')">Judging Form</a></li>
-<li><a href="#">Contents2</a></li>
 </ul>
 </li>
-<li id="Safety"><a href="Javascript:open('Safety')"><img src="https://static.igem.org/mediawiki/2014/9/96/LinkCTC_safetyon.png" class="contList" alt="https://static.igem.org/mediawiki/2014/9/93/LinkCTC_safetyopen.png"></a>
+<li id="Safety"><a href="Javascript:loadContent('Safety-block','top')"><img src="https://static.igem.org/mediawiki/2014/9/96/LinkCTC_safetyon.png" class="contList"></a>
-<ul class="subContList">
-<li><a href="#">Contents1</a></li>
-<li><a href="#">Contents2</a></li>
-</ul>
 </li>
 <li id="Attribution"><a href="Javascript:open('Attribution')"><img src="https://static.igem.org/mediawiki/2014/3/38/LinkCTC_attributionon.png" class="contList" alt="https://static.igem.org/mediawiki/2014/9/92/LinkCTC_attributionopen.png"></a>
 <ul class="subContList">
-<li><a href="#">Contents1</a></li>
+<li><a href="Javascript:loadContent('Attribution-block','Attribution-1')">Attribution</a></li>
-<li><a href="#">Contents2</a></li>
+<li><a href="Javascript:loadContent('Attribution-block','Attribution-2')">Acknowledgement</a></li>
+<li><a href="Javascript:loadContent('Attribution-block','Attribution-3')">Sponsors</a></li>
 </ul>
 </li>
 <li id="Humanpractice"><a href="Javascript:open('Humanpractice')"><img src="https://static.igem.org/mediawiki/2014/5/5e/LinkCTC_humanon.png" class="contList" alt="https://static.igem.org/mediawiki/2014/c/c9/LinkCTC_humanopen.png"></a>
 <ul class="subContList">
-<li><a href="#">Contents1</a></li>
+<li><a href="Javascript:loadContent('Humanpractice-block','Humanpractice-1')">Human Practice</a></li>
-<li><a href="#">Contents2</a></li>
+<li><a href="Javascript:loadContent('Humanpractice-block','Humanpractice-2')">Ethics</a></li>
 </ul>
 </li>
-<li id="Team"><a href="Javascript:loadContent('Team-top')"><img src="https://static.igem.org/mediawiki/2014/8/83/LinkCTC_teamon.png" class="contList" alt="https://static.igem.org/mediawiki/2014/3/31/LinkCTC_teamopen.png"></a>
+<li id="Team"><a href="Javascript:loadContent('Team-top','top')"><img src="https://static.igem.org/mediawiki/2014/8/83/LinkCTC_teamon.png" class="contList" alt="https://static.igem.org/mediawiki/2014/3/31/LinkCTC_teamopen.png"></a>
 <ul class="subContList">
 <li><a href="#">Contents1</a></li>
@@ Line 63: / Line 59: @@
 </div>
 <div id="contentsBody">
-	<img src="https://static.igem.org/mediawiki/2014/7/7d/Igemut-tokyo2014.png" id="RightTop" />
+	<a href="https://igem.org/Main_Page" target="_blank"><img src="https://static.igem.org/mediawiki/2014/7/7d/Igemut-tokyo2014.png" id="RightTop" /></a>
 	<div class="pageContentsBox">
 		<div id="pageContents">
+			<div id = "loading-img" style="display:block">
+			<img src="https://static.igem.org/mediawiki/2014/2/24/Loading-35.gif" width="100px" style="margin-left:400px;padding-top:100px;" />
+			</div>
 			<!-- Projectここから -->
 			<div id = "Project-block" style = "display:none">
-				<!-- Project background -->
+			</div>
-				<div id = "Project-1">
+			<!-- Result -->
-				<img src = "https://static.igem.org/mediawiki/2014/d/db/Sub_background.png" class = "contTitle"/>
+			<div id = "Result-block" style = "display:none">
-				<p>The word, "counter", will remind you the machine with which you can count the number of the objects, such as people and cars. Some people familiar with electronic circuits may remind the logic circuit. In any case, both are the memory device of the order of inputs and  are important for our lives.<br /><br />Cellular counters also memorize the number of events. In nature, there are tellomere length regulation, cell aggregation and so on. <br />Ari et al constructed the cellular counter with synthetic biological approach, termed the riboregulated transcriptional cascade (RTC) counter[1].The state transition occurs after arabinose induction (Fig. 1). The system is regulated by riboregulators. The Biobrick part of this cellular counter has already existed, which was constructed by Tokyo-Nokogen 2009 and was named BBa_K225002, BBa_K25003 [2]. <br />In order to expand the function of this counter, we added reset system. The reset sysytem realizes the movement from any state to the initial state after the particular input and is expected to movement from down stream states to upstream states by respective inputs, which we regard as automaton. Our counter utilizes the regulation system of sigma factor and anti-σ factor as the key of its resetting mechanism. In addition to reset function, the number of states can be increased more easily, because what we have to think is only the combination of σ and anti-σ factor, that is, we do not have to find new polymerase. Moreover, because there is orthonal relation between sigma and anti-sigma factor, we can choose combinations which help us avoid crosstalk problem. <br /><br />Fig. 1 The concept of RTC counter. After first, second and third induction of arabinose, the state of cells moves from 0 to 1, 1 to 2 and 2 to 3, respectively.<br /><br />[1]Ari E. Friedland, et al., Science 324, 1199 (2009). <br />[2]https://2009.igem.org/Team:Tokyo-Nokogen/Parts</p>
+			</div>
-				</div>
+			<!-- Lab Data -->
-				<!-- Project background -->
+			<div id = "Lab-block" style = "display:none;">
-				<!-- Project σ-memory -->
+			</div>
-				<div id = "Project-2">
+			<!-- Modeling -->
-				<img src = "https://static.igem.org/mediawiki/2014/e/e4/Sub_memory.png" class = "contTitle" />
+			<div id = "Modeling-block" style = "display:none;">
-				<h3>σ factor</h3>
+			</div>
-				<p>σ factor is a subunit of RNA polymerase related to promoter recognitions. There are two types of sigma factors, one that is housekeeping (expressed constantly) and another that is expressed under some conditions. It is possible to deliver genes of sigma factors that are not derived from some species to another species, so we can use many kinds of σ factor. Which promoter RNA polymerase tends to bind to is decided by which σ factor RNA polymerase is bound. Especially some σ factors promote only transcription of a specific promoters. Therefore if we choose a set of sigma factors and promoters skillfully, we can control transcription without crosstalk. Here, “without crosstalk” means every σ factor in the set promotes only transcription of the cognate promoter.<br />Anti-σ is also a protein which is related to transcriptional control by σ factors. Anti-σ prevents σ factors from binding to RNA polymerase. Similarly as σ factor, there are many kinds of anti-σ and we can choose a set of σ factors, promoters, and anti-σs which has no crosstalk. In our project, we use such a set of σ factors, promoters, and anti-σs.</p>
+			<!-- Achievement -->
-				<h3>σ-memory construction</h3>
+			<div id = "Achievement-block" style = "display:none;">
-				<p>This is the construction of our σ-memory. If input A exists, σ factor is expressed. Then the positive feedback circuits of σ factor starts producing σ factor, and consequently σ factor will remain. Though if input B exists, anti-σ is expressed and the positive feedback circuits is inhibited. Both σ factor and anti-σ are subjects to degradation, so all of them are decomposed after some time and σ-memory returns to original state.<br />We can regard existence/absence of σ factor as 1/0 of memory, and this values of memory can switch by input A or input B. Using the promoter which is cognate to the σ factor, the information whether the value of memory is 1 or 0 can be derived. For example, consider the circuits on the right. The repotor is expressed if and only if σ-memory's value is 1 (i.e. σ factor exists).<br />In addition, no crosstalk sets of σ factors, promoters, and anti-σs enable us to make  multi-σ-memory gene circuits. To make the explanation easier, consider the case in which E. coli has two σ memories, σA-memory and σB-memory. The value of σA-memory change from 0 to 1 if input A1 exists and change from 1 to 0 if inputs B1 exists. Also the value of σB-memory change from 0 to 1 if input A2 exists and change from 1 to 0 if input B2 exists. If the four input A1, B1, A2, and B2 have no crosstalk, σA-memory and σB-memory also have no crosstalk. For example, when input A1 exists, only the value of σA-memory change from 0 to 1 since σA promotes only transcription from PσA (promoter that is cognate to σA), and has no effect on σB. The same is true of input B1 and anti-σA (anti-σ that is cognate to σA). So it can be confidently said that E. coli has two σ memories.<br />Since this construction has a positive feedback, leakage of promoter may be a serious problem. If the promoter has leakage and σ factor is expressed when input A dose not exist, this error is enlarged by positive feedback. However if the leakage of anti-σ is satisfactory large, σ factor dose not produced from positive feedback when leak of σ factor occurs. Therefore leakage is not an obstacle of our project if we choose the promoter skillfully.
+			</div>
-				<h3>σ-memory and automaton</h3>
+			<!-- Safety -->
-				<p>Speaking easily, an automaton is referred to what has states and transition rules. Every state has a transition rule, say it is decided what is the next state if an input exists. For example, the automaton on the right has five states, 0, 1, 2, 3, 4, and transition rules are represented by arrows. If the current state is 0 and the input is b, the next state is 2. Transition rules having loops or feedbacks are allowed.<br />σ-memory can be considered an automaton. Every tuple of σ-memories' value (say, (1, 1, 0, 1, 0)) corresponds to a state. It is possible to use one σ-memory as an inputs of another σ-memory. For example, consider the gene circuit on the right.The value of σA-memory( a σ-memory which uses σA in the construction as a σ factor)  change from 0 to 1 if arabinose exists, also the value of σB-memory change from 0 to 1 if the value of σA-memory is 1 and IPTG exists. (This condition can be written in terms of mathematical logic, namely AND(σA, IPTG)) These gene circuits can be considered as the automaton on the right. Gene circuits which work as logic circuits (says AND, OR, NAND, etc...) have been designed.</p>
+			<div id = "Safety-block" style = "display:none;">
-				</div>
+			</div>
-				<!-- Project σ-memory -->
+			<!-- Attribution -->
-				<!-- Project Application -->
+			<div id = "Attribution-block" style = "display:none;">
-				<div id = "Project-3">
+			</div>
-				<img src = "https://static.igem.org/mediawiki/2014/9/93/Sub_appli.png" class = "contTitle" />
+			<!-- Human Practice -->
+			<div id = "Humanpractice-block" style = "display:none;">
-				<h3>resettable counter by σ-memory</h3>
-				<p>Since a counter is one kind of automata, σ-memory can be applied for making resettable counters. It can count the number of induction events of arabinose, and express a reporter correspond to each states. In addition, the count can be reset by IPTG induction.<br />2-counter is a counter that can count up to 2, and is an automaton that has three states, 0, 1, and 2. This automaton can be constructed by σ-memory as following. Two kinds of σ factor are necessary for constructing the 2-counter, so for the convenience we will call these two kinds of σ factors σA and σB. In state 0, both σA and σB do not exist (i.e. σA-memory and σB-memory is 0). In state 1, only σA exists and in states 2, both σ factors exist. The input is the induction of arabinose or IPTG, and the transition rules with it are showed in the Figure.<br />A gene circuit that realizes this automaton can be represented as following. The value of σA changes from 0 to 1 after arabinose induction (i.e. σA is expressed when arabinose exists). This change corresponds to the transition from state from 0 to 1.The value of σB-memory changes from 0 to 1 when both arabinose and σA exist. 【説明不足】Therefore the AND function is necessary. This change corresponds to the transition from state 1 to state 2. Both the value of σA-memory and σB-memory change from 1 to 0 after IPTG induction (i.e. anti-σA and σB is expressed when IPTG exist).</p>
-				<h3>riboregulator</h3>
-				<p>According to the discussion in 2-1, it is necessary to use AND function for the construction of a resettable counter. Therefore a riboregulator is used for the realization of the AND function.<br />A riboregulator is a post-transcriptional regulation system composed of two kinds of RNAs. Cis- repressed mRNA(crRNA) forms a stem-loop and its ribosomal binding site (RBS) is covered. Consequently the gene coded in cis-repressed mRNA isn't translated. However if trans-activating RNA (taRNA) exist, crRNA and taRNA are hybridized and RBS gets exposed and translation starts.<br />Namely, the gene coded in crRNA is expressed only when both crRNA and taRNA is transcripted. Therefore this system can be considered as the AND function. For example, consider the gene circuit, Plac-taRNA-d.term-Pbad-cr-RBS-GFP-d.term.(cr is the sequence in crRNA which binds to RBS) If and only if both IPTG and arabinose exists, GFP is expressed. Therefore, the riboregulator can combine two promoters and produce AND functions</p>
-				<h3>resettable counter construction</h3>
-				<p>The construction of our resettable counter is explained here.<br /><br />σ20 and σ11 are used as σA and σB as mentioned above respectively. The promoters correspond to σ20 and σ11 are Pσ20 and Pσ11, and the anti-σs are anti-20 and anti-11 respectively. GFP is used as a reporter. <br />At first, both the value of σ20-memory and σ11-memory are 0. Only the crRNA coding σ20, which is at downstream of constitutive promoter is translated. After the first induction of arabinose, taRNA at the downstream of PBAD is transcribed and the crRNA coding σ20 is translated, and the value of σ20-memory changes from 0 to 1. Since σ20 exists, crRNA coding σ11 at the downstream of Pσ20 is transcribed. After the second induction of arabinose, taRNA is transcribed and σ11 is expressed, and the value of σ11-memory changes from 0 to 1. At this time, GFP at the downstream of Pσ11 is expressed and we can check the count as 2.<br />After an induction of IPTG, anti-σ20 and σ11 are expressed, and the value of σ20-memory and σ11-memory will be 0. Therefore the count is reset.<br /><br />Since there are many kinds of pair of σ factors and cognate promoters which have no crosstalk, n-counter can be made by the same way. Besides, the construction of 2-counter can be simplified.<br />This simplified counter is made by only one σ factor. It works as the same way as original 2-counter unill 1 count. After 1 count, crRNA coding GFP is transcribed at the downstream of Pσ. Therefore when the next arabinose induction occurs, GFP is translated. This expression can be considered a report of 2 count. This simplified counter can be also extended to n count. Simplified counter can count up to larger numbers compared to the original counter even when the same number of σ factors are used, but cannot be reset from their final count. We did experiments on this simplified counter.</p>
-				</div>
-				<!-- Project application -->
 			</div>
 			<!-- Teamここから -->
@@ Line 113: / Line 103: @@
 						</div>
 						<div class = "member">
-							<p class = "name">MASAKI ONO</p>
+							<p class = "name">YOICHI IRIE</p>
 							<img src = "https://static.igem.org/mediawiki/2014/6/6a/Irie_comb.png" class ="member-detail" />
 							<dl class = "profile">
 								<dt>Name</dt>
-								<dd>Masaki Ono</dd>
+								<dd>Yoichi Irie</dd>
 								<dt>Belong to</dt>
-								<dd>The University of Tokyo, Literature, Sophmore</dd>
+								<dd>The University of Tokyo, Arts and Sciences</dd>
 								<dt>Job</dt>
-								<dd>Modeling</dd>
+								<dd>Team leader</dd>
 							</dl>
-							<p class ="comment">I want to be a doctor.</p>
+							<p class ="comment">My dream is to make me robuster against stress caused by iGEM in synthetic biology!</p>
 						</div>
 					</div>
@@ Line 137: / Line 127: @@
 						</div>
 						<div class = "member">
-							<p class = "name">MASAKI ONO</p>
+							<p class = "name" style="font-size:35px;">CRISTIAN DAVID PENA MARINEZ</p>
 							<img src = "https://static.igem.org/mediawiki/2014/f/f1/Chris_comb.png" class ="member-detail" />
 							<dl class = "profile">
 								<dt>Name</dt>
-								<dd>Masaki Ono</dd>
+								<dd>Cristian David Pena Martinez</dd>
 								<dt>Belong to</dt>
-								<dd>The University of Tokyo, Literature, Sophmore</dd>
+								<dd>Department of Regulatory Biology, Faculty of Science, Saitama University</dd>
 								<dt>Job</dt>
-								<dd>Modeling</dd>
+								<dd>Design</dd>
 							</dl>
-							<p class ="comment">I want to be a doctor.</p>
+							<p class ="comment">"The only concrete proof of the human existence is: poetry" - Luis Cardoza y Aragon</p>
 						</div>
 					</div>
@@ Line 167: / Line 157: @@
 								<dd>Masaki Ono</dd>
 								<dt>Belong to</dt>
-								<dd>The University of Tokyo, Literature, Sophmore</dd>
+								<dd>The University of Tokyo, Arts and Sciences, Sophmore</dd>
-								<dt>Job</dt>
-								<dd>Modeling</dd>
-							</dl>
-							<p class ="comment">I want to be a doctor.</p>
-						</div>
-					</div>
-				</div>
-				<div class = "element-item">
-					<div class = "thumbnail">
-						<img src = "https://static.igem.org/mediawiki/2014/a/a1/Ishikawa.png" class ="member-thumbnail" />
-					</div>
-					<div class = "detail">
-						<div class = "meta">
-							<p>iGEM UT-Tokyo</p>
-							<img src = "https://static.igem.org/mediawiki/2014/9/98/Ui_close_40.png" class = "closebutton" />
-						</div>
-						<div class = "member">
-							<p class = "name">MASAKI ONO</p>
-							<img src = "https://static.igem.org/mediawiki/2014/7/78/Cocoa_comb.png" class ="member-detail" />
-							<dl class = "profile">
-								<dt>Name</dt>
-								<dd>Masaki Ono</dd>
-								<dt>Belong to</dt>
-								<dd>The University of Tokyo, Literature, Sophmore</dd>
 								<dt>Job</dt>
 								<dd>Modeling</dd>
@@ Line 209: / Line 175: @@
 						</div>
 						<div class = "member">
-							<p class = "name">MASAKI ONO</p>
+							<p class = "name">YUMENO KOGA</p>
 							<img src = "https://static.igem.org/mediawiki/2014/6/63/Koga_comb.png" class ="member-detail" />
 							<dl class = "profile">
 								<dt>Name</dt>
-								<dd>Masaki Ono</dd>
+								<dd>Yumeno Koga</dd>
 								<dt>Belong to</dt>
-								<dd>The University of Tokyo, Literature, Sophmore</dd>
+								<dd>Department of Lifescience & Medical Bioscience, School of Advanced Science and Engineering, Waseda University</dd>
 								<dt>Job</dt>
-								<dd>Modeling</dd>
+								<dd>Presenter</dd>
 							</dl>
-							<p class ="comment">I want to be a doctor.</p>
+							<p class ="comment">I'm a  "wasejo".</p>
 						</div>
 					</div>
@@ Line 233: / Line 199: @@
 						</div>
 						<div class = "member">
-							<p class = "name">MASAKI ONO</p>
+							<p class = "name">KENTO NAKAMURA</p>
 							<img src = "https://static.igem.org/mediawiki/2014/8/87/Nakamura_comb.png" class ="member-detail" />
 							<dl class = "profile">
 								<dt>Name</dt>
-								<dd>Masaki Ono</dd>
+								<dd>Kento Nakamura</dd>
 								<dt>Belong to</dt>
-								<dd>The University of Tokyo, Literature, Sophmore</dd>
+								<dd>College of Arts and Sciences, The University of Tokyo</dd>
 								<dt>Job</dt>
-								<dd>Modeling</dd>
+								<dd>Experimenter, Presenter</dd>
 							</dl>
-							<p class ="comment">I want to be a doctor.</p>
+							<p class ="comment">"I think, therefore I am" by R. Descartes<br />"I multiply, therefore I am" by E.coli <br />"I hope so..." by Experimenter</p>
 						</div>
 					</div>
@@ Line 257: / Line 223: @@
 						</div>
 						<div class = "member">
-							<p class = "name">MASAKI ONO</p>
+							<p class = "name">SOH NAKASHIMA</p>
 							<img src = "https://static.igem.org/mediawiki/2014/7/74/Dolicas_comb.png" class ="member-detail" />
 							<dl class = "profile">
 								<dt>Name</dt>
-								<dd>Masaki Ono</dd>
+								<dd>Soh Nakashima</dd>
 								<dt>Belong to</dt>
-								<dd>The University of Tokyo, Literature, Sophmore</dd>
+								<dd>College of Arts and Sciences, The University of Tokyo</dd>
 								<dt>Job</dt>
-								<dd>Modeling</dd>
+								<dd>Experimenter</dd>
 							</dl>
-							<p class ="comment">I want to be a doctor.</p>
+							<p class ="comment">follow me on Twitter(<a href="https://twitter.com/dolicas_" target="_blank" >@dolicas_</a>)</p>
 						</div>
 					</div>
@@ Line 281: / Line 247: @@
 						</div>
 						<div class = "member">
-							<p class = "name">MASAKI ONO</p>
+							<p class = "name">MANABU NISHIURA</p>
 							<img src = "https://static.igem.org/mediawiki/2014/2/22/Nishiura_comb.png" class ="member-detail" />
 							<dl class = "profile">
 								<dt>Name</dt>
-								<dd>Masaki Ono</dd>
+								<dd>Manabu Nishiura</dd>
 								<dt>Belong to</dt>
-								<dd>The University of Tokyo, Literature, Sophmore</dd>
+								<dd>The University of Tokyo, Arts and Sciences</dd>
 								<dt>Job</dt>
-								<dd>Modeling</dd>
+								<dd>Modeling,Presenter</dd>
 							</dl>
-							<p class ="comment">I want to be a doctor.</p>
+							<p class ="comment"> I am a Feynman Diagram.</p>
 						</div>
 					</div>
@@ Line 305: / Line 271: @@
 						</div>
 						<div class = "member">
-							<p class = "name">MASAKI ONO</p>
+							<p class = "name">YOSHIKI OKESAKU</p>
 							<img src = "https://static.igem.org/mediawiki/2014/d/d0/Oke_comb.png" class ="member-detail" />
 							<dl class = "profile">
 								<dt>Name</dt>
-								<dd>Masaki Ono</dd>
+								<dd>Yoshiki Okesaku</dd>
 								<dt>Belong to</dt>
-								<dd>The University of Tokyo, Literature, Sophmore</dd>
+								<dd> Department of Physics, Graduate School of Science, The University of Tokyo</dd>
 								<dt>Job</dt>
-								<dd>Modeling</dd>
+								<dd>Design(DOKATA)</dd>
 							</dl>
-							<p class ="comment">I want to be a doctor.</p>
+							<p class ="comment">I am majoring in the MDS theory. The MDS theory can describe how our universe had begun and will end.</p>
 						</div>
 					</div>
@@ Line 329: / Line 295: @@
 						</div>
 						<div class = "member">
-							<p class = "name">MASAKI ONO</p>
+							<p class = "name">HAJIME TAKEMURA</p>
 							<img src = "https://static.igem.org/mediawiki/2014/3/35/Takemura_comb.png" class ="member-detail" />
 							<dl class = "profile">
 								<dt>Name</dt>
-								<dd>Masaki Ono</dd>
+								<dd>Hajime Takemura</dd>
 								<dt>Belong to</dt>
-								<dd>The University of Tokyo, Literature, Sophmore</dd>
+								<dd>The University of Tokyo, Junior,Faculty of Pharmaceutical  Science</dd>
 								<dt>Job</dt>
-								<dd>Modeling</dd>
+								<dd>Experiment</dd>
 							</dl>
-							<p class ="comment">I want to be a doctor.</p>
+							<p class ="comment">I want to make a new medicine.</p>
 						</div>
 					</div>
@@ Line 353: / Line 319: @@
 						</div>
 						<div class = "member">
-							<p class = "name">MASAKI ONO</p>
+							<p class = "name">KENTARO TARA</p>
 							<img src = "https://static.igem.org/mediawiki/2014/e/ef/Tara_comb.png" class ="member-detail" />
 							<dl class = "profile">
 								<dt>Name</dt>
-								<dd>Masaki Ono</dd>
+								<dd>Kentaro Tara</dd>
 								<dt>Belong to</dt>
-								<dd>The University of Tokyo, Literature, Sophmore</dd>
+								<dd>Arts and Sciences, The University of Tokyo</dd>
 								<dt>Job</dt>
-								<dd>Modeling</dd>
+								<dd>Experiment, Modeling</dd>
 							</dl>
-							<p class ="comment">I want to be a doctor.</p>
+							<p class ="comment">Maps relax me.</p>
 						</div>
 					</div>
@@ Line 377: / Line 343: @@
 						</div>
 						<div class = "member">
-							<p class = "name">MASAKI ONO</p>
+							<p class = "name">YUEWEN DING</p>
 							<img src = "https://static.igem.org/mediawiki/2014/0/0e/Tei_comb.png" class ="member-detail" />
 							<dl class = "profile">
 								<dt>Name</dt>
-								<dd>Masaki Ono</dd>
+								<dd>Yuewen Ding</dd>
 								<dt>Belong to</dt>
-								<dd>The University of Tokyo, Literature, Sophmore</dd>
+								<dd>Faculty of Pharmaceutical  Science, The university of Tokyo</dd>
 								<dt>Job</dt>
-								<dd>Modeling</dd>
+								<dd>Public relation</dd>
 							</dl>
-							<p class ="comment">I want to be a doctor.</p>
+							<p class ="comment">Eating is sweeter than Life.</p>
 						</div>
 					</div>
@@ Line 401: / Line 367: @@
 						</div>
 						<div class = "member">
-							<p class = "name">MASAKI ONO</p>
+							<p class = "name">YOSHIHIKO TOMOFUJI</p>
 							<img src = "https://static.igem.org/mediawiki/2014/2/21/Tomohuji_comb.png" class ="member-detail" />
 							<dl class = "profile">
 								<dt>Name</dt>
-								<dd>Masaki Ono</dd>
+								<dd>Yoshihiko Tomofuji</dd>
 								<dt>Belong to</dt>
-								<dd>The University of Tokyo, Literature, Sophmore</dd>
+								<dd>The university of Tokyo, faculty of medicine</dd>
 								<dt>Job</dt>
-								<dd>Modeling</dd>
+								<dd>E.coli</dd>
 							</dl>
-							<p class ="comment">I want to be a doctor.</p>
+							<p class ="comment">I'm a E.coli.</p>
 						</div>
 					</div>
@@ Line 425: / Line 391: @@
 						</div>
 						<div class = "member">
-							<p class = "name">MASAKI ONO</p>
+							<p class = "name">KOHTA TOSHIMITSU</p>
 							<img src = "https://static.igem.org/mediawiki/2014/7/7c/Toshimitsu_comb.png" class ="member-detail" />
 							<dl class = "profile">
 								<dt>Name</dt>
-								<dd>Masaki Ono</dd>
+								<dd>Kohta Toshimitsu</dd>
 								<dt>Belong to</dt>
-								<dd>The University of Tokyo, Literature, Sophmore</dd>
+								<dd>Faculty of Pharmacy,　Keio University</dd>
 								<dt>Job</dt>
-								<dd>Modeling</dd>
+								<dd>Adviser</dd>
 							</dl>
-							<p class ="comment">I want to be a doctor.</p>
+							<p class ="comment">I'm so sick of pipetting.</p>
 						</div>
 					</div>
@@ Line 449: / Line 415: @@
 						</div>
 						<div class = "member">
-							<p class = "name">MASAKI ONO</p>
+							<p class = "name">KEISUKE TSUKADA</p>
 							<img src = "https://static.igem.org/mediawiki/2014/0/0b/Tsukada_comb.png" class ="member-detail" />
 							<dl class = "profile">
 								<dt>Name</dt>
-								<dd>Masaki Ono</dd>
+								<dd>Keisuke Tsukada</dd>
 								<dt>Belong to</dt>
-								<dd>The University of Tokyo, Literature, Sophmore</dd>
+								<dd>Department of Biophysics and Biochemistry , Faculty of Science, The University of Tokyo</dd>
 								<dt>Job</dt>
-								<dd>Modeling</dd>
+								<dd>Experiment, Modeling</dd>
 							</dl>
-							<p class ="comment">I want to be a doctor.</p>
+							<p class ="comment">itininnmaenokenkyuusyaninaretaraiina</p>
 						</div>
 					</div>
@@ Line 473: / Line 439: @@
 						</div>
 						<div class = "member">
-							<p class = "name">MASAKI ONO</p>
+							<p class = "name">YUTO YAMANAKA</p>
 							<img src = "https://static.igem.org/mediawiki/2014/2/26/Yamanaka_comb.png" class ="member-detail" />
 							<dl class = "profile">
 								<dt>Name</dt>
-								<dd>Masaki Ono</dd>
+								<dd>Yuto Yamanaka</dd>
 								<dt>Belong to</dt>
-								<dd>The University of Tokyo, Literature, Sophmore</dd>
+								<dd>Department of Electrical Engineering and Bioscience, School of Advanced Science and Engineering, Waseda University</dd>
 								<dt>Job</dt>
-								<dd>Modeling</dd>
+								<dd>Experimenter, Presenter</dd>
+							</dl>
+							<p class ="comment">I like Falcon tubes.</p>
+						</div>
+					</div>
+				</div>
+				<div class = "element-item">
+					<div class = "thumbnail">
+						<img src = "https://static.igem.org/mediawiki/2014/3/3e/Sumi.png" class ="member-thumbnail" />
+					</div>
+					<div class = "detail">
+						<div class = "meta">
+							<p>iGEM UT-Tokyo</p>
+							<img src = "https://static.igem.org/mediawiki/2014/9/98/Ui_close_40.png" class = "closebutton" />
+						</div>
+						<div class = "member">
+							<p class = "name">SHUNSUKE SUMI</p>
+							<img src = "https://static.igem.org/mediawiki/2014/e/e8/Sumi_comb.png" class ="member-detail" />
+							<dl class = "profile">
+								<dt>Name</dt>
+								<dd>Shunsuke Sumi</dd>
+								<dt>Belong to</dt>
+								<dd>Department of medicine, Faculty of medicine, The Jikei University School of Medicine</dd>
+								<dt>Job</dt>
+								<dd>tree</dd>
+							</dl>
+							<p class ="comment">a tree has no emotion.</p>
+						</div>
+					</div>
+				</div>
+				<div class = "element-item">
+					<div class = "thumbnail">
+						<img src = "https://static.igem.org/mediawiki/2014/3/34/Yoshikawa%281st%29.png" class ="member-thumbnail" />
+					</div>
+					<div class = "detail">
+						<div class = "meta">
+							<p>iGEM UT-Tokyo</p>
+							<img src = "https://static.igem.org/mediawiki/2014/9/98/Ui_close_40.png" class = "closebutton" />
+						</div>
+						<div class = "member">
+							<p class = "name">NARUKI YOSHIKAWA</p>
+							<img src = "https://static.igem.org/mediawiki/2014/d/de/Yoshikawa%281st%29_comb.png" class ="member-detail" />
+							<dl class = "profile">
+								<dt>Name</dt>
+								<dd>Naruki Yoshikawa</dd>
+								<dt>Belong to</dt>
+								<dd>College of Arts and Sciences, The University of Tokyo</dd>
+								<dt>Job</dt>
+								<dd>Experimenter</dd>
+							</dl>
+							<p class ="comment">Three billion devices run Java, but my devices don't.</p>
+						</div>
+					</div>
+				</div>
+				<div class = "element-item">
+					<div class = "thumbnail">
+						<img src = "https://static.igem.org/mediawiki/2014/9/95/Hiura.png" class ="member-thumbnail" />
+					</div>
+					<div class = "detail">
+						<div class = "meta">
+							<p>iGEM UT-Tokyo</p>
+							<img src = "https://static.igem.org/mediawiki/2014/9/98/Ui_close_40.png" class = "closebutton" />
+						</div>
+						<div class = "member">
+							<p class = "name">NOBUHIRO HIURA</p>
+							<img src = "https://static.igem.org/mediawiki/2014/e/ea/Hiura_comb.png" class ="member-detail" />
+							<dl class = "profile">
+								<dt>Name</dt>
+								<dd>Nobuhiro Hiura</dd>
+								<dt>Belong to</dt>
+								<dd>Department of Regulatory Biology, Faculty of Science, Saitama University</dd>
+								<dt>Job</dt>
+								<dd>Experimenter</dd>
+							</dl>
+							<p class ="comment">Let's go to Saitama, Japan!</p>
+						</div>
+					</div>
+				</div>
+				<div class = "element-item">
+					<div class = "thumbnail">
+						<img src = "https://static.igem.org/mediawiki/2014/8/8c/Tsuboi.png" class ="member-thumbnail" />
+					</div>
+					<div class = "detail">
+						<div class = "meta">
+							<p>iGEM UT-Tokyo</p>
+							<img src = "https://static.igem.org/mediawiki/2014/9/98/Ui_close_40.png" class = "closebutton" />
+						</div>
+						<div class = "member">
+							<p class = "name">HIROKI TSUBOI</p>
+							<img src = "https://static.igem.org/mediawiki/2014/b/b6/Tsuboi_comb.png" class ="member-detail" />
+							<dl class = "profile">
+								<dt>Name</dt>
+								<dd>Hiroki Tsuboi</dd>
+								<dt>Belong to</dt>
+								<dd>Waseda University, School of Fundamental Science and Engineering, Computer Science and Engineering</dd>
+								<dt>Job</dt>
+								<dd>Web</dd>
+							</dl>
+							<p class ="comment">After wiki freeze, I will make other Web Site</p>
+						</div>
+					</div>
+				</div>
+				<div class = "element-item">
+					<div class = "thumbnail">
+						<img src = "https://static.igem.org/mediawiki/2014/2/2b/Kobari.png" class ="member-thumbnail" />
+					</div>
+					<div class = "detail">
+						<div class = "meta">
+							<p>iGEM UT-Tokyo</p>
+							<img src = "https://static.igem.org/mediawiki/2014/9/98/Ui_close_40.png" class = "closebutton" />
+						</div>
+						<div class = "member">
+							<p class = "name">SHIGETAKA KOBARI</p>
+							<img src = "https://static.igem.org/mediawiki/2014/f/fa/Kobari_comb.png" class ="member-detail" />
+							<dl class = "profile">
+								<dt>Name</dt>
+								<dd>Shigetaka Kobari</dd>
+								<dt>Belong to</dt>
+								<dd>The university of Tokyo, faculty of medicine</dd>
+								<dt>Job</dt>
+								<dd>tree</dd>
+							</dl>
+							<p class ="comment">a tree has no emotion.</p>
+						</div>
+					</div>
+				</div>
+				<div class = "element-item">
+					<div class = "thumbnail">
+						<img src = "https://static.igem.org/mediawiki/2014/a/a1/Ishikawa.png" class ="member-thumbnail" />
+					</div>
+					<div class = "detail">
+						<div class = "meta">
+							<p>iGEM UT-Tokyo</p>
+							<img src = "https://static.igem.org/mediawiki/2014/9/98/Ui_close_40.png" class = "closebutton" />
+						</div>
+						<div class = "member">
+							<p class = "name">MASATO ISHIKAWA</p>
+							<img src = "https://static.igem.org/mediawiki/2014/e/e9/Ishikawa_comb.png" class ="member-detail" />
+							<dl class = "profile">
+								<dt>Name</dt>
+								<dd>Masato Ishikawa</dd>
+								<dt>Belong to</dt>
+								<dd>College of Arts and Sciences, The University of Tokyo</dd>
+								<dt>Job</dt>
+								<dd>Presenter</dd>
+							</dl>
+							<p class ="comment">I want to study pharmacy.</p>
+						</div>
+					</div>
+				</div>
+				<div class = "element-item">
+					<div class = "thumbnail">
+						<img src = "https://static.igem.org/mediawiki/2014/c/c6/Osawa.png" class ="member-thumbnail" />
+					</div>
+					<div class = "detail">
+						<div class = "meta">
+							<p>iGEM UT-Tokyo</p>
+							<img src = "https://static.igem.org/mediawiki/2014/9/98/Ui_close_40.png" class = "closebutton" />
+						</div>
+						<div class = "member">
+							<p class = "name">MASAYUKI OSAWA</p>
+							<img src = "https://static.igem.org/mediawiki/2014/1/12/Osawa_comb.png" class ="member-detail" />
+							<dl class = "profile">
+								<dt>Name</dt>
+								<dd>Masayuki Osawa</dd>
+								<dt>Belong to</dt>
+								<dd>Department of medicine, Faculty of medicine, The Jikei University School of Medicine</dd>
+								<dt>Job</dt>
+								<dd>Woodkeeper</dd>
+							</dl>
+							<p class ="comment">I take care of crazy tree.</p>
+						</div>
+					</div>
+				</div>
+				<div class = "element-item">
+					<div class = "thumbnail">
+						<img src = "https://static.igem.org/mediawiki/2014/5/53/Tube.png" class ="member-thumbnail" />
+					</div>
+					<div class = "detail">
+						<div class = "meta">
+							<p>iGEM UT-Tokyo</p>
+							<img src = "https://static.igem.org/mediawiki/2014/9/98/Ui_close_40.png" class = "closebutton" />
+						</div>
+						<div class = "member">
+							<p class = "name">TAKEHUMI YOSHIKAWA</p>
+							<img src = "https://static.igem.org/mediawiki/2014/b/b1/Tube_comb.png" class ="member-detail" />
+							<dl class = "profile">
+								<dt>Name</dt>
+								<dd>Takehumi Yoshikawa</dd>
+								<dt>Belong to</dt>
+								<dd>Department of Bioinformatics and Systems Biology, Faculty of Science, The University of Tokyo</dd>
+								<dt>Job</dt>
+								<dd>Experiment Leader</dd>
+							</dl>
+							<p class ="comment">I want to be Spe1.</p>
+						</div>
+					</div>
+				</div>
+				<div class = "element-item">
+					<div class = "thumbnail">
+						<img src = "https://static.igem.org/mediawiki/2014/d/d4/Ito.png" class ="member-thumbnail" />
+					</div>
+					<div class = "detail">
+						<div class = "meta">
+							<p>iGEM UT-Tokyo</p>
+							<img src = "https://static.igem.org/mediawiki/2014/9/98/Ui_close_40.png" class = "closebutton" />
+						</div>
+						<div class = "member">
+							<p class = "name">ATSUKI ITO</p>
+							<img src = "https://static.igem.org/mediawiki/2014/1/1d/Ito_comb.png" class ="member-detail" />
+							<dl class = "profile">
+								<dt>Name</dt>
+								<dd>Atsuki Ito</dd>
+								<dt>Belong to</dt>
+								<dd>Department of Bioscience, Faculty of Engineering,Tokyo University of Science</dd>
+								<dt>Job</dt>
+								<dd>Experimenter</dd>
+							</dl>
+							<p class ="comment">kimuwaipu,azinai...</p>
+						</div>
+					</div>
+				</div>
+				<div class = "element-item">
+					<div class = "thumbnail">
+						<img src = "https://static.igem.org/mediawiki/2014/3/3e/Prof_wakamoto.png" class ="member-thumbnail" />
+					</div>
+					<div class = "detail">
+						<div class = "meta">
+							<p>iGEM UT-Tokyo</p>
+							<img src = "https://static.igem.org/mediawiki/2014/9/98/Ui_close_40.png" class = "closebutton" />
+						</div>
+						<div class = "member">
+							<p class = "name">PROF.WAKAMOTO</p>
+							<img src = "https://static.igem.org/mediawiki/2014/f/fb/Prof_wakamoto_comb.png" class ="member-detail" />
+							<dl class = "profile">
+								<dt>Name</dt>
+								<dd>Yuichi Wakamoto</dd>
+								<dt>Belong to</dt>
+								<dd>Center for Complex Systems Biology The University of Tokyo, Dapartment of Basic Science, Graduate School of Arts and Sciences, The University of Tokyo</dd>
+								<dt>Job</dt>
+								<dd>Instructor</dd>
 							</dl>
-							<p class ="comment">I want to be a doctor.</p>
 						</div>
 					</div>
@@ Line 489: / Line 694: @@
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 			</div>
-			<!-- Teamのトップここまで -->
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 				<a href="Javascript:bttop()"><img src="https://static.igem.org/mediawiki/2014/0/06/Topbutton.png" /></a>
 			</div>
-			<!-- 上へ戻るボタン -->
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Team:UT-Tokyo/CTCD/Content

From 2014.igem.org

Latest revision as of 03:02, 18 October 2014