Team:USyd-Australia/Project/ResultsConclusion
From 2014.igem.org
Results of Components
pUS201 - Low Copy Backbone
Characterization of the low-copy backbone was unfortunately unsuccessful. Despite careful planning and verification of correct insertion and sequence the low-copy backbone displayed high copy replication characteristics. See the pUS201 Parts page for more info.
pUS203 - Controllable Integrase
Without pUS201 the experimental design had to adapt. The controllable Integrase system from pUS203 should ideally be in a low copy backbone but the the iGEM supplied low-copy plasmid was not an option as the anti-biotic resistance interfered with the experimental set up. At this point in time we did not have time to alter the experiment to such a degree as to accommodate the change. Instead we set about validating the controllable integrase system in the submission plasmid to try and determine whether it was producing integrase but the initial experiment failed and follow up experiments to troubleshoot problems were not possible due to time constraints. See the pUS203 Parts page for more info.