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From 2014.igem.org

After validation of the low-copy plasmid was unsuccessful (see the pUS201 Parts page for more info) our plan had to alter slightly. Cloning the controllable integrase into the iGEM supplied low-copy plasmid was not an option as the anti-biotic resistance interfered with the experimental set up and we did not have time to alter the experiment to such a degree as to accommodate the change. Instead we set about validating the controllable integrase system in the submission plasmid to try and determine whether it was producing integrase but the initial experiment failed and follow up experiments to troubleshoot problems were not possible due to time constraints (see the (see the pUS203 Parts page for more). The cassette was functionally proven although it did not express the aeBlue gene but did confer Gm resistance. After sequencing we have found that a deletion near the end of the part seem to have been the cause for the absence of blue from the colonies expressing the cassete.