Team:USyd-Australia/Project/Design

From 2014.igem.org

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<img src="https://static.igem.org/mediawiki/2014/c/c4/USyd-Australia_Integron_Design-Produce_Cassettes.png" width="60%" align="center"><br> <p>Gene Cassettes are the carriers of genetic information in our design. They are small, modular chunks of DNA containing a gene flanked by AttC sites. Cassettes, unlike plasmids, are non-replicative and so do not require extra genes to modulate or initiate replication. To create a cassette simply requires the use of primers to create half AttC sites at either end and circularizing the product through <a href="https://2014.igem.org/Team:USyd-Australia/Project/Protocols#gibson">Gibson Assembly</a> or in other ways.</p>
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<img src="https://static.igem.org/mediawiki/2014/c/c4/USyd-Australia_Integron_Design-Produce_Cassettes.png" width="60%" align="center"><br> <p>Gene Cassettes are the carriers of genetic information in our design. They are small, modular chunks of DNA containing a gene flanked by AttC sites. Cassettes, unlike plasmids, are non-replicative and so do not require extra genes to modulate or initiate replication. To create a cassette simply requires the use of primers to create half AttC sites at either end and circularizing the product through <a href="https://2014.igem.org/Team:USyd-Australia/Project/Protocols#gibson">Gibson Assembly</a> or in other ways.</p><br><h2> </h2>
<img src="https://static.igem.org/mediawiki/2014/1/1f/USyd-Australia_Integron_Design-Express_Integrase.png" width="60%">
<img src="https://static.igem.org/mediawiki/2014/1/1f/USyd-Australia_Integron_Design-Express_Integrase.png" width="60%">
<img src="https://static.igem.org/mediawiki/2014/5/55/USyd-Australia_Integron_design-add_cassettes.png" width="60%">
<img src="https://static.igem.org/mediawiki/2014/5/55/USyd-Australia_Integron_design-add_cassettes.png" width="60%">

Revision as of 17:12, 17 October 2014

iGEM_Link


Project Design


Gene Cassettes are the carriers of genetic information in our design. They are small, modular chunks of DNA containing a gene flanked by AttC sites. Cassettes, unlike plasmids, are non-replicative and so do not require extra genes to modulate or initiate replication. To create a cassette simply requires the use of primers to create half AttC sites at either end and circularizing the product through Gibson Assembly or in other ways.


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