Team:USyd-Australia/Notebook/gBlock

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Revision as of 02:32, 18 October 2014 by Andy.Bachler (Talk | contribs)

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gBlocks

All gBlocks were supplied by IDT.

gBlocks are DNA chunks up to 2kb made from a sequence template.

NameSequence (5'-3')Purpose
IntI1CCGTTTTTTTGGATGGAGTGAAACGATGGCGATTGCAATAGCTAGCTAAAGGAGGATTCATATGAAAACCGCCACTGCGCCGTTACCACCGCTGCGTTCGGTCAAGGTTCTGGACCAGTTGCGTGAGCGCATACGCTACTTGCATTACTCCCTCCGAACCGAACAGGCTTACGTTAACTGGGTTCGTGCCTTCATCCGTTTCCACGGTGTGCGTCACCCGGCAACCTTGGGCAGCAGCGAAGTCGAGGCATTTCTGTCCTGGCTGGCGAACGAGCGCAAGGTTTCGGTCTCCACGCATCGTCAGGCATTGGCGGCCTTGCTGTTCTTCTACGGCAAGGTGCTGTGCACGGATCTGCCCTGGCTTCAGGAGATCGGAAGACCTCGGCCGTCGCGGCGCTTGCCGGTGGTGCTGACCCCGGATGAAGTGGTTCGCATCCTCGGTTTTCTGGAAGGCGAGCATCGTTTGTTCGCCCAGCTTCTGTATGGAACGGGCATGCGGATCAGTGAGGGTTTGCAACTGCGGGTCAAGGATCTGGATTTCGATCACGGCACGATCATCGTGCGGGAGGGCAAGGGCTCCAAGGATCGGGCCTTGATGTTACCCGAGAGCTTGGCACCCAGCCTGCGCGAGCAGCTGTCGCGTGCACGGGCATGGTGGCTGAAGGACCAGGCCGAGGGCCGCAGCGGCGTTGCGCTTCCCGACGCCCTTGAGCGGAAGTATCCGCGCGCCGGGCATTCCTGGCCGTGGTTCTGGGTTTTTGCGCAGCACACGCATTCGACCGATCCACGGAGCGGTGTCGTGCGTCGCCATCACATGTATGACCAGACCTTTCAGCGCGCCTTCAAACGTGCCGTAGAACAAGCAGGCATCACGAAGCCCGCCACACCGCACACCCTCCGCCACTCGTTCGCGACGGCCTTGCTCCGCAGCGGTTACGACATTCGAACCGTGCAGGATCTGCTCGGCCATTCCGACGTCTCTACGACGATGATTTACACGCATGTGCTGAAAGTTGGCGGTGCCGGAGTGCGCTCACCGCTTGATGCGCTGCCGCCCCTCACTTCGGAGAGGTAGTACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAGGGCAAGGTGTCATo be inserted into SamR construct as part of the controllable integrase expression system
AttC-aeBlue-aacC1 TTTGAATTCGCGGCCGCTTCTAGAGTACCGAGGGACAGACTACCAACTCACAGCTAGCGCCTAACAATTCGTCCAAGCCGACGCCGCTTCGCGGCGCGGCTTAACTCAGGTGTTAGGCAAAGGAGGTTATTGATATGGCTTCACTGGTTAAAAAAGACATGTGCATCAAAATGACGATGGAAGGAACAGTAAACGGTCACCATTTCAAGTGTGTAGGAGAAGGCGAAGGCAAACCATTTGAAGGGACCCAGGTGGAAAAGATACGCATCACTGAAGGTGGGCCCTTACCATTTGCGTATGATATTTTGGCCCCTTGTTGCATGTATGGCAGTAAAACCTTCATTAAGCATGTGTCGGGTATTCCGGATTACTTTAAGGAGTCTTTTCCTGAGGGCTTTACCTGGGAAAGAACACAAATCTTCGAGGATGGCGGCTATCTCACCATACACCAGGACACGAGCCTTCAGGGTAATAATTTTATTTTCAAAGTTAATGTCATCGGTGCCAACTTCCCTGCAAACGGTCCCGTGATGCAGAAAAAAACAGCTGGATGGGAACCGTGCGTTGAGATGCTTTATCCGCGGGACGGCGTCCTGTGTGGTCAGAGCCTGATGGCCCTGAAATGCACTGATGGCAATCATCTGACGTCCCACCTGCGCACTACCTATCGTTCTCGCAAGCCATCCAATGCAGTTAACATGCCGGAATTTCATTTTGGGGATCATCGCATTGAGATTTTGAAAGCTGAACAAGGTAAATTTTATGAACAATACGAGTCAGCGGTGGCCCGTTACTGTGAGGCGGCACCGAGTAAATTAGGGCATCACTAATAAAGGATCCAAAGGAGGCTCAAGTATGGGCATCATTCGCACATGTAGGCTCGGCCCTGACCAAGTCAAATCCATGCGGGCTGCTCTTGATCTTTTCGGTCGTGAGTTCGGAGACGTAGCCACCTACTCCCAACATCAGCCGGACTCCGATTACCTCGGGAACTTGCTCCGTAGTAAGACATTCATCGCGCTTGCTGCCTTCGACCAAGAAGCGGTTGTTGGCGCTCTCGCGGCTTACGTTCTGCCCAGGTTTGAGCAGCCGCGTAGTGAGATCTATATCTATGATCTCGCAGTCTCCGGCGAGCACCGGAGGCAGGGCATTGCCACCGCGCTCATCAATCTCCTCAAGCATGAGGCCAACGCGCTTGGTGCTTATGTGATCTACGTGCAAGCAGATTACGGTGACGATCCCGCAGTGGCTCTCTATACAAAGTTGGGCATACGGGAAGAAGTGATGCACTTTGATATCGACCCAAGTACCGCCACCTAATAATACTAGTAGCGGCCGCTGCAGCCCTo be used as a template for the creation of gene cassettes with antibiotic resistance and phenotypic markers

With thanks to:

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