Team:USyd-Australia/Notebook/gBlock

From 2014.igem.org

(Difference between revisions)
Line 8: Line 8:
<tr><td>
<tr><td>
<p>All gBlocks were supplied by IDT.</p>
<p>All gBlocks were supplied by IDT.</p>
-
<p>gBlocks are DNA chunks up to 2kb made from a sequence template. 3 were reconstituted in 10&#956;L TE buffer </p>
+
<p>gBlocks are DNA chunks up to 2kb made from a sequence template.</p>
<p></p>
<p></p>
</td></tr>
</td></tr>
Line 16: Line 16:
<tr><th width="20%">Name</th><th width="60%">Sequence (5'-3')</th><th width="20%">Purpose</th></tr>
<tr><th width="20%">Name</th><th width="60%">Sequence (5'-3')</th><th width="20%">Purpose</th></tr>
 +
<tr><td><a name="IntI1.2"></a>IntI1</td><td style="word-wrap: break-word">CCGTTTTTTTGGATGGAGTGAAACGATGGCGATTGCAATAGCTAGCTAAAGGAGGATTCATATGAAAACCGCCACTGCGCCGTTACCACCGCTGCGTTCGGTCAAGGTTCTGGACCAGTTGCGTGAGCGCATACGCTACTTGCATTACTCCCTCCGAACCGAACAGGCTTACGTTAACTGGGTTCGTGCCTTCATCCGTTTCCACGGTGTGCGTCACCCGGCAACCTTGGGCAGCAGCGAAGTCGAGGCATTTCTGTCCTGGCTGGCGAACGAGCGCAAGGTTTCGGTCTCCACGCATCGTCAGGCATTGGCGGCCTTGCTGTTCTTCTACGGCAAGGTGCTGTGCACGGATCTGCCCTGGCTTCAGGAGATCGGAAGACCTCGGCCGTCGCGGCGCTTGCCGGTGGTGCTGACCCCGGATGAAGTGGTTCGCATCCTCGGTTTTCTGGAAGGCGAGCATCGTTTGTTCGCCCAGCTTCTGTATGGAACGGGCATGCGGATCAGTGAGGGTTTGCAACTGCGGGTCAAGGATCTGGATTTCGATCACGGCACGATCATCGTGCGGGAGGGCAAGGGCTCCAAGGATCGGGCCTTGATGTTACCCGAGAGCTTGGCACCCAGCCTGCGCGAGCAGCTGTCGCGTGCACGGGCATGGTGGCTGAAGGACCAGGCCGAGGGCCGCAGCGGCGTTGCGCTTCCCGACGCCCTTGAGCGGAAGTATCCGCGCGCCGGGCATTCCTGGCCGTGGTTCTGGGTTTTTGCGCAGCACACGCATTCGACCGATCCACGGAGCGGTGTCGTGCGTCGCCATCACATGTATGACCAGACCTTTCAGCGCGCCTTCAAACGTGCCGTAGAACAAGCAGGCATCACGAAGCCCGCCACACCGCACACCCTCCGCCACTCGTTCGCGACGGCCTTGCTCCGCAGCGGTTACGACATTCGAACCGTGCAGGATCTGCTCGGCCATTCCGACGTCTCTACGACGATGATTTACACGCATGTGCTGAAAGTTGGCGGTGCCGGAGTGCGCTCACCGCTTGATGCGCTGCCGCCCCTCACTTCGGAGAGGTAGTACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAGGGCAAGGTGTCA</td><td>To be inserted into SamR construct as part of the controllable integrase expression system</td></tr>
<tr><td><a name="IntI1.2"></a>IntI1</td><td style="word-wrap: break-word">CCGTTTTTTTGGATGGAGTGAAACGATGGCGATTGCAATAGCTAGCTAAAGGAGGATTCATATGAAAACCGCCACTGCGCCGTTACCACCGCTGCGTTCGGTCAAGGTTCTGGACCAGTTGCGTGAGCGCATACGCTACTTGCATTACTCCCTCCGAACCGAACAGGCTTACGTTAACTGGGTTCGTGCCTTCATCCGTTTCCACGGTGTGCGTCACCCGGCAACCTTGGGCAGCAGCGAAGTCGAGGCATTTCTGTCCTGGCTGGCGAACGAGCGCAAGGTTTCGGTCTCCACGCATCGTCAGGCATTGGCGGCCTTGCTGTTCTTCTACGGCAAGGTGCTGTGCACGGATCTGCCCTGGCTTCAGGAGATCGGAAGACCTCGGCCGTCGCGGCGCTTGCCGGTGGTGCTGACCCCGGATGAAGTGGTTCGCATCCTCGGTTTTCTGGAAGGCGAGCATCGTTTGTTCGCCCAGCTTCTGTATGGAACGGGCATGCGGATCAGTGAGGGTTTGCAACTGCGGGTCAAGGATCTGGATTTCGATCACGGCACGATCATCGTGCGGGAGGGCAAGGGCTCCAAGGATCGGGCCTTGATGTTACCCGAGAGCTTGGCACCCAGCCTGCGCGAGCAGCTGTCGCGTGCACGGGCATGGTGGCTGAAGGACCAGGCCGAGGGCCGCAGCGGCGTTGCGCTTCCCGACGCCCTTGAGCGGAAGTATCCGCGCGCCGGGCATTCCTGGCCGTGGTTCTGGGTTTTTGCGCAGCACACGCATTCGACCGATCCACGGAGCGGTGTCGTGCGTCGCCATCACATGTATGACCAGACCTTTCAGCGCGCCTTCAAACGTGCCGTAGAACAAGCAGGCATCACGAAGCCCGCCACACCGCACACCCTCCGCCACTCGTTCGCGACGGCCTTGCTCCGCAGCGGTTACGACATTCGAACCGTGCAGGATCTGCTCGGCCATTCCGACGTCTCTACGACGATGATTTACACGCATGTGCTGAAAGTTGGCGGTGCCGGAGTGCGCTCACCGCTTGATGCGCTGCCGCCCCTCACTTCGGAGAGGTAGTACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAGGGCAAGGTGTCA</td><td>To be inserted into SamR construct as part of the controllable integrase expression system</td></tr>
 +
 +
<tr><td><a name="AttC-aeBlue-aacC1 "></a>AttC-aeBlue-aacC1 </td><td style="word-wrap: break-word">TTTGAATTCGCGGCCGCTTCTAGAGTACCGAGGGACAGACTACCAACTCACAGCTAGCGCCTAACAATTCGTCCAAGCCGACGCCGCTTCGCGGCGCGGCTTAACTCAGGTGTTAGGCAAAGGAGGTTATTGATATGGCTTCACTGGTTAAAAAAGACATGTGCATCAAAATGACGATGGAAGGAACAGTAAACGGTCACCATTTCAAGTGTGTAGGAGAAGGCGAAGGCAAACCATTTGAAGGGACCCAGGTGGAAAAGATACGCATCACTGAAGGTGGGCCCTTACCATTTGCGTATGATATTTTGGCCCCTTGTTGCATGTATGGCAGTAAAACCTTCATTAAGCATGTGTCGGGTATTCCGGATTACTTTAAGGAGTCTTTTCCTGAGGGCTTTACCTGGGAAAGAACACAAATCTTCGAGGATGGCGGCTATCTCACCATACACCAGGACACGAGCCTTCAGGGTAATAATTTTATTTTCAAAGTTAATGTCATCGGTGCCAACTTCCCTGCAAACGGTCCCGTGATGCAGAAAAAAACAGCTGGATGGGAACCGTGCGTTGAGATGCTTTATCCGCGGGACGGCGTCCTGTGTGGTCAGAGCCTGATGGCCCTGAAATGCACTGATGGCAATCATCTGACGTCCCACCTGCGCACTACCTATCGTTCTCGCAAGCCATCCAATGCAGTTAACATGCCGGAATTTCATTTTGGGGATCATCGCATTGAGATTTTGAAAGCTGAACAAGGTAAATTTTATGAACAATACGAGTCAGCGGTGGCCCGTTACTGTGAGGCGGCACCGAGTAAATTAGGGCATCACTAATAAAGGATCCAAAGGAGGCTCAAGTATGGGCATCATTCGCACATGTAGGCTCGGCCCTGACCAAGTCAAATCCATGCGGGCTGCTCTTGATCTTTTCGGTCGTGAGTTCGGAGACGTAGCCACCTACTCCCAACATCAGCCGGACTCCGATTACCTCGGGAACTTGCTCCGTAGTAAGACATTCATCGCGCTTGCTGCCTTCGACCAAGAAGCGGTTGTTGGCGCTCTCGCGGCTTACGTTCTGCCCAGGTTTGAGCAGCCGCGTAGTGAGATCTATATCTATGATCTCGCAGTCTCCGGCGAGCACCGGAGGCAGGGCATTGCCACCGCGCTCATCAATCTCCTCAAGCATGAGGCCAACGCGCTTGGTGCTTATGTGATCTACGTGCAAGCAGATTACGGTGACGATCCCGCAGTGGCTCTCTATACAAAGTTGGGCATACGGGAAGAAGTGATGCACTTTGATATCGACCCAAGTACCGCCACCTAATAATACTAGTAGCGGCCGCTGCAGCCC</td><td>To be used as a template for the creation of gene cassettes with antibiotic resistance and phenotypic markers</td></tr>
</table>
</table>
</html>
</html>
{{Team:USyd-Australia/Footer}}
{{Team:USyd-Australia/Footer}}

Revision as of 02:32, 18 October 2014

iGEM_Link


gBlocks

All gBlocks were supplied by IDT.

gBlocks are DNA chunks up to 2kb made from a sequence template.

NameSequence (5'-3')Purpose
IntI1CCGTTTTTTTGGATGGAGTGAAACGATGGCGATTGCAATAGCTAGCTAAAGGAGGATTCATATGAAAACCGCCACTGCGCCGTTACCACCGCTGCGTTCGGTCAAGGTTCTGGACCAGTTGCGTGAGCGCATACGCTACTTGCATTACTCCCTCCGAACCGAACAGGCTTACGTTAACTGGGTTCGTGCCTTCATCCGTTTCCACGGTGTGCGTCACCCGGCAACCTTGGGCAGCAGCGAAGTCGAGGCATTTCTGTCCTGGCTGGCGAACGAGCGCAAGGTTTCGGTCTCCACGCATCGTCAGGCATTGGCGGCCTTGCTGTTCTTCTACGGCAAGGTGCTGTGCACGGATCTGCCCTGGCTTCAGGAGATCGGAAGACCTCGGCCGTCGCGGCGCTTGCCGGTGGTGCTGACCCCGGATGAAGTGGTTCGCATCCTCGGTTTTCTGGAAGGCGAGCATCGTTTGTTCGCCCAGCTTCTGTATGGAACGGGCATGCGGATCAGTGAGGGTTTGCAACTGCGGGTCAAGGATCTGGATTTCGATCACGGCACGATCATCGTGCGGGAGGGCAAGGGCTCCAAGGATCGGGCCTTGATGTTACCCGAGAGCTTGGCACCCAGCCTGCGCGAGCAGCTGTCGCGTGCACGGGCATGGTGGCTGAAGGACCAGGCCGAGGGCCGCAGCGGCGTTGCGCTTCCCGACGCCCTTGAGCGGAAGTATCCGCGCGCCGGGCATTCCTGGCCGTGGTTCTGGGTTTTTGCGCAGCACACGCATTCGACCGATCCACGGAGCGGTGTCGTGCGTCGCCATCACATGTATGACCAGACCTTTCAGCGCGCCTTCAAACGTGCCGTAGAACAAGCAGGCATCACGAAGCCCGCCACACCGCACACCCTCCGCCACTCGTTCGCGACGGCCTTGCTCCGCAGCGGTTACGACATTCGAACCGTGCAGGATCTGCTCGGCCATTCCGACGTCTCTACGACGATGATTTACACGCATGTGCTGAAAGTTGGCGGTGCCGGAGTGCGCTCACCGCTTGATGCGCTGCCGCCCCTCACTTCGGAGAGGTAGTACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAGGGCAAGGTGTCATo be inserted into SamR construct as part of the controllable integrase expression system
AttC-aeBlue-aacC1 TTTGAATTCGCGGCCGCTTCTAGAGTACCGAGGGACAGACTACCAACTCACAGCTAGCGCCTAACAATTCGTCCAAGCCGACGCCGCTTCGCGGCGCGGCTTAACTCAGGTGTTAGGCAAAGGAGGTTATTGATATGGCTTCACTGGTTAAAAAAGACATGTGCATCAAAATGACGATGGAAGGAACAGTAAACGGTCACCATTTCAAGTGTGTAGGAGAAGGCGAAGGCAAACCATTTGAAGGGACCCAGGTGGAAAAGATACGCATCACTGAAGGTGGGCCCTTACCATTTGCGTATGATATTTTGGCCCCTTGTTGCATGTATGGCAGTAAAACCTTCATTAAGCATGTGTCGGGTATTCCGGATTACTTTAAGGAGTCTTTTCCTGAGGGCTTTACCTGGGAAAGAACACAAATCTTCGAGGATGGCGGCTATCTCACCATACACCAGGACACGAGCCTTCAGGGTAATAATTTTATTTTCAAAGTTAATGTCATCGGTGCCAACTTCCCTGCAAACGGTCCCGTGATGCAGAAAAAAACAGCTGGATGGGAACCGTGCGTTGAGATGCTTTATCCGCGGGACGGCGTCCTGTGTGGTCAGAGCCTGATGGCCCTGAAATGCACTGATGGCAATCATCTGACGTCCCACCTGCGCACTACCTATCGTTCTCGCAAGCCATCCAATGCAGTTAACATGCCGGAATTTCATTTTGGGGATCATCGCATTGAGATTTTGAAAGCTGAACAAGGTAAATTTTATGAACAATACGAGTCAGCGGTGGCCCGTTACTGTGAGGCGGCACCGAGTAAATTAGGGCATCACTAATAAAGGATCCAAAGGAGGCTCAAGTATGGGCATCATTCGCACATGTAGGCTCGGCCCTGACCAAGTCAAATCCATGCGGGCTGCTCTTGATCTTTTCGGTCGTGAGTTCGGAGACGTAGCCACCTACTCCCAACATCAGCCGGACTCCGATTACCTCGGGAACTTGCTCCGTAGTAAGACATTCATCGCGCTTGCTGCCTTCGACCAAGAAGCGGTTGTTGGCGCTCTCGCGGCTTACGTTCTGCCCAGGTTTGAGCAGCCGCGTAGTGAGATCTATATCTATGATCTCGCAGTCTCCGGCGAGCACCGGAGGCAGGGCATTGCCACCGCGCTCATCAATCTCCTCAAGCATGAGGCCAACGCGCTTGGTGCTTATGTGATCTACGTGCAAGCAGATTACGGTGACGATCCCGCAGTGGCTCTCTATACAAAGTTGGGCATACGGGAAGAAGTGATGCACTTTGATATCGACCCAAGTACCGCCACCTAATAATACTAGTAGCGGCCGCTGCAGCCCTo be used as a template for the creation of gene cassettes with antibiotic resistance and phenotypic markers

With thanks to:

Retrieved from "http://2014.igem.org/Team:USyd-Australia/Notebook/gBlock"