Team:USyd-Australia/Notebook/gBlock

From 2014.igem.org

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<p>All gBlocks were supplied by IDT.</p>
<p>All gBlocks were supplied by IDT.</p>
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<p>gBlocks are DNA chunks up to 2kb made from a sequence template. 3 were reconstituted in 10&#956;L TE buffer </p>
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<p>gBlocks are DNA chunks up to 2kb made from a sequence template.</p>
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<p></p>
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<table id="gBlockTable" style="table-layout: fixed; width: 100%">
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<table id="gBlockTable">
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<tr><th width="10%">Name</th><th>Sequence (5'-3')</th>><th>Purpose</th></tr>
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<tr id="gBlockTableRow"><th id="gBlockTableHeading" width="10%">Name</th><th id="gBlockTableHeading" width="45%">Sequence (5'-3')</th><th  id="gBlockTableHeading" width="5%">Size</th><th id="gBlockTableHeading" width="20%">Purpose</th></tr>
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<tr><td><a name="IntI1.2"></a>IntI1.2</td><td style="word-wrap: break-word">CCGTTTTTTTGGATGGAGTGAAACGATGGCGATTGCAATAGCTAGCTAAAGGAGGATTCATATGAAAACCGCCACTGCGCCGTTACCACCGCTGCGTTCGGTCAAGGTTCTGGACCAGTTGCGTGAGCGCATACGCTACTTGCATTACTCCCTCCGAACCGAACAGGCTTACGTTAACTGGGTTCGTGCCTTCATCCGTTTCCACGGTGTGCGTCACCCGGCAACCTTGGGCAGCAGCGAAGTCGAGGCATTTCTGTCCTGGCTGGCGAACGAGCGCAAGGTTTCGGTCTCCACGCATCGTCAGGCATTGGCGGCCTTGCTGTTCTTCTACGGCAAGGTGCTGTGCACGGATCTGCCCTGGCTTCAGGAGATCGGAAGACCTCGGCCGTCGCGGCGCTTGCCGGTGGTGCTGACCCCGGATGAAGTGGTTCGCATCCTCGGTTTTCTGGAAGGCGAGCATCGTTTGTTCGCCCAGCTTCTGTATGGAACGGGCATGCGGATCAGTGAGGGTTTGCAACTGCGGGTCAAGGATCTGGATTTCGATCACGGCACGATCATCGTGCGGGAGGGCAAGGGCTCCAAGGATCGGGCCTTGATGTTACCCGAGAGCTTGGCACCCAGCCTGCGCGAGCAGCTGTCGCGTGCACGGGCATGGTGGCTGAAGGACCAGGCCGAGGGCCGCAGCGGCGTTGCGCTTCCCGACGCCCTTGAGCGGAAGTATCCGCGCGCCGGGCATTCCTGGCCGTGGTTCTGGGTTTTTGCGCAGCACACGCATTCGACCGATCCACGGAGCGGTGTCGTGCGTCGCCATCACATGTATGACCAGACCTTTCAGCGCGCCTTCAAACGTGCCGTAGAACAAGCAGGCATCACGAAGCCCGCCACACCGCACACCCTCCGCCACTCGTTCGCGACGGCCTTGCTCCGCAGCGGTTACGACATTCGAACCGTGCAGGATCTGCTCGGCCATTCCGACGTCTCTACGACGATGATTTACACGCATGTGCTGAAAGTTGGCGGTGCCGGAGTGCGCTCACCGCTTGATGCGCTGCCGCCCCTCACTTCGGAGAGGTAGTACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAGGGCAAGGTGTCA</td><td>58.2C</td><td>Insert into SamR construct to be used as part of the controllable integrase expression system</td></tr>
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<tr id="gBlockTableRow"><td id="gBlockTableColumn"><a name="IntI1.2"></a>IntI1</td><td id="gBlockTableColumn" style="word-wrap: break-word; font-size: 8px;">CCGTTTTTTTGGATGGAGTGAAACGATGGCGATTGCAATAGCTAGCTAAAGGAGGATTCATATGAAAACCGCCACTGCGCCGTTACCACCGCTGCGTTCGGTCAAGGTTCTGGACCAGTTGCGTGAGCGCATACGCTACTTGCATTACTCCCTCCGAACCGAACAGGCTTACGTTAACTGGGTTCGTGCCTTCATCCGTTTCCACGGTGTGCGTCACCCGGCAACCTTGGGCAGCAGCGAAGTCGAGGCATTTCTGTCCTGGCTGGCGAACGAGCGCAAGGTTTCGGTCTCCACGCATCGTCAGGCATTGGCGGCCTTGCTGTTCTTCTACGGCAAGGTGCTGTGCACGGATCTGCCCTGGCTTCAGGAGATCGGAAGACCTCGGCCGTCGCGGCGCTTGCCGGTGGTGCTGACCCCGGATGAAGTGGTTCGCATCCTCGGTTTTCTGGAAGGCGAGCATCGTTTGTTCGCCCAGCTTCTGTATGGAACGGGCATGCGGATCAGTGAGGGTTTGCAACTGCGGGTCAAGGATCTGGATTTCGATCACGGCACGATCATCGTGCGGGAGGGCAAGGGCTCCAAGGATCGGGCCTTGATGTTACCCGAGAGCTTGGCACCCAGCCTGCGCGAGCAGCTGTCGCGTGCACGGGCATGGTGGCTGAAGGACCAGGCCGAGGGCCGCAGCGGCGTTGCGCTTCCCGACGCCCTTGAGCGGAAGTATCCGCGCGCCGGGCATTCCTGGCCGTGGTTCTGGGTTTTTGCGCAGCACACGCATTCGACCGATCCACGGAGCGGTGTCGTGCGTCGCCATCACATGTATGACCAGACCTTTCAGCGCGCCTTCAAACGTGCCGTAGAACAAGCAGGCATCACGAAGCCCGCCACACCGCACACCCTCCGCCACTCGTTCGCGACGGCCTTGCTCCGCAGCGGTTACGACATTCGAACCGTGCAGGATCTGCTCGGCCATTCCGACGTCTCTACGACGATGATTTACACGCATGTGCTGAAAGTTGGCGGTGCCGGAGTGCGCTCACCGCTTGATGCGCTGCCGCCCCTCACTTCGGAGAGGTAGTACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAGGGCAAGGTGTCA</td><td id="gBlockTableColumn">1121 bp</td><td id="gBlockTableColumn">To be inserted into SamR construct as part of the controllable integrase expression system</td></tr>
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<tr id="gBlockTableRow"><td id="gBlockTableColumn"><a name="AttC-aeBlue-aacC1 "></a>AttC-aeBlue-aacC1 </td><td  id="gBlockTableColumn" style="word-wrap: break-word; font-size: 8px;">TTTGAATTCGCGGCCGCTTCTAGAGTACCGAGGGACAGACTACCAACTCACAGCTAGCGCCTAACAATTCGTCCAAGCCGACGCCGCTTCGCGGCGCGGCTTAACTCAGGTGTTAGGCAAAGGAGGTTATTGATATGGCTTCACTGGTTAAAAAAGACATGTGCATCAAAATGACGATGGAAGGAACAGTAAACGGTCACCATTTCAAGTGTGTAGGAGAAGGCGAAGGCAAACCATTTGAAGGGACCCAGGTGGAAAAGATACGCATCACTGAAGGTGGGCCCTTACCATTTGCGTATGATATTTTGGCCCCTTGTTGCATGTATGGCAGTAAAACCTTCATTAAGCATGTGTCGGGTATTCCGGATTACTTTAAGGAGTCTTTTCCTGAGGGCTTTACCTGGGAAAGAACACAAATCTTCGAGGATGGCGGCTATCTCACCATACACCAGGACACGAGCCTTCAGGGTAATAATTTTATTTTCAAAGTTAATGTCATCGGTGCCAACTTCCCTGCAAACGGTCCCGTGATGCAGAAAAAAACAGCTGGATGGGAACCGTGCGTTGAGATGCTTTATCCGCGGGACGGCGTCCTGTGTGGTCAGAGCCTGATGGCCCTGAAATGCACTGATGGCAATCATCTGACGTCCCACCTGCGCACTACCTATCGTTCTCGCAAGCCATCCAATGCAGTTAACATGCCGGAATTTCATTTTGGGGATCATCGCATTGAGATTTTGAAAGCTGAACAAGGTAAATTTTATGAACAATACGAGTCAGCGGTGGCCCGTTACTGTGAGGCGGCACCGAGTAAATTAGGGCATCACTAATAAAGGATCCAAAGGAGGCTCAAGTATGGGCATCATTCGCACATGTAGGCTCGGCCCTGACCAAGTCAAATCCATGCGGGCTGCTCTTGATCTTTTCGGTCGTGAGTTCGGAGACGTAGCCACCTACTCCCAACATCAGCCGGACTCCGATTACCTCGGGAACTTGCTCCGTAGTAAGACATTCATCGCGCTTGCTGCCTTCGACCAAGAAGCGGTTGTTGGCGCTCTCGCGGCTTACGTTCTGCCCAGGTTTGAGCAGCCGCGTAGTGAGATCTATATCTATGATCTCGCAGTCTCCGGCGAGCACCGGAGGCAGGGCATTGCCACCGCGCTCATCAATCTCCTCAAGCATGAGGCCAACGCGCTTGGTGCTTATGTGATCTACGTGCAAGCAGATTACGGTGACGATCCCGCAGTGGCTCTCTATACAAAGTTGGGCATACGGGAAGAAGTGATGCACTTTGATATCGACCCAAGTACCGCCACCTAATAATACTAGTAGCGGCCGCTGCAGCCC</td><td id="gBlockTableColumn">1347 bp</td><td id="gBlockTableColumn">To be used as a template for the creation of gene cassettes with antibiotic resistance and phenotypic markers</td></tr>
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<tr id="gBlockTableRow"><td id="gBlockTableColumn"><a name="LacI-lacO-pLac-attI"></a>LacI-lacO-pLac-attI  </td><td id="gBlockTableColumn" style="word-wrap: break-word; font-size: 8px;">GGGAATTCAAATCTAGAGACACCATCGAATGGTGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGAAGGAGGTGAATATGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTCAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTAATAAGCGCAAAAAAAAAACCCCGCCCCTGACAGGGCGGGGTTTTTTTTCAACGCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAGGCAAAACTAGTAAACTGCAGGG</td><td id="gBlockTableColumn">1403 bp</td><td id="gBlockTableColumn">To be used as an AttI site with a Lac operon to allow controllable expression</td></tr>
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{{Team:USyd-Australia/Footer}}
{{Team:USyd-Australia/Footer}}

Latest revision as of 03:54, 18 October 2014

iGEM_Link


gBlocks

All gBlocks were supplied by IDT.

gBlocks are DNA chunks up to 2kb made from a sequence template.

NameSequence (5'-3')SizePurpose
IntI1CCGTTTTTTTGGATGGAGTGAAACGATGGCGATTGCAATAGCTAGCTAAAGGAGGATTCATATGAAAACCGCCACTGCGCCGTTACCACCGCTGCGTTCGGTCAAGGTTCTGGACCAGTTGCGTGAGCGCATACGCTACTTGCATTACTCCCTCCGAACCGAACAGGCTTACGTTAACTGGGTTCGTGCCTTCATCCGTTTCCACGGTGTGCGTCACCCGGCAACCTTGGGCAGCAGCGAAGTCGAGGCATTTCTGTCCTGGCTGGCGAACGAGCGCAAGGTTTCGGTCTCCACGCATCGTCAGGCATTGGCGGCCTTGCTGTTCTTCTACGGCAAGGTGCTGTGCACGGATCTGCCCTGGCTTCAGGAGATCGGAAGACCTCGGCCGTCGCGGCGCTTGCCGGTGGTGCTGACCCCGGATGAAGTGGTTCGCATCCTCGGTTTTCTGGAAGGCGAGCATCGTTTGTTCGCCCAGCTTCTGTATGGAACGGGCATGCGGATCAGTGAGGGTTTGCAACTGCGGGTCAAGGATCTGGATTTCGATCACGGCACGATCATCGTGCGGGAGGGCAAGGGCTCCAAGGATCGGGCCTTGATGTTACCCGAGAGCTTGGCACCCAGCCTGCGCGAGCAGCTGTCGCGTGCACGGGCATGGTGGCTGAAGGACCAGGCCGAGGGCCGCAGCGGCGTTGCGCTTCCCGACGCCCTTGAGCGGAAGTATCCGCGCGCCGGGCATTCCTGGCCGTGGTTCTGGGTTTTTGCGCAGCACACGCATTCGACCGATCCACGGAGCGGTGTCGTGCGTCGCCATCACATGTATGACCAGACCTTTCAGCGCGCCTTCAAACGTGCCGTAGAACAAGCAGGCATCACGAAGCCCGCCACACCGCACACCCTCCGCCACTCGTTCGCGACGGCCTTGCTCCGCAGCGGTTACGACATTCGAACCGTGCAGGATCTGCTCGGCCATTCCGACGTCTCTACGACGATGATTTACACGCATGTGCTGAAAGTTGGCGGTGCCGGAGTGCGCTCACCGCTTGATGCGCTGCCGCCCCTCACTTCGGAGAGGTAGTACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAGGGCAAGGTGTCA1121 bpTo be inserted into SamR construct as part of the controllable integrase expression system
AttC-aeBlue-aacC1 TTTGAATTCGCGGCCGCTTCTAGAGTACCGAGGGACAGACTACCAACTCACAGCTAGCGCCTAACAATTCGTCCAAGCCGACGCCGCTTCGCGGCGCGGCTTAACTCAGGTGTTAGGCAAAGGAGGTTATTGATATGGCTTCACTGGTTAAAAAAGACATGTGCATCAAAATGACGATGGAAGGAACAGTAAACGGTCACCATTTCAAGTGTGTAGGAGAAGGCGAAGGCAAACCATTTGAAGGGACCCAGGTGGAAAAGATACGCATCACTGAAGGTGGGCCCTTACCATTTGCGTATGATATTTTGGCCCCTTGTTGCATGTATGGCAGTAAAACCTTCATTAAGCATGTGTCGGGTATTCCGGATTACTTTAAGGAGTCTTTTCCTGAGGGCTTTACCTGGGAAAGAACACAAATCTTCGAGGATGGCGGCTATCTCACCATACACCAGGACACGAGCCTTCAGGGTAATAATTTTATTTTCAAAGTTAATGTCATCGGTGCCAACTTCCCTGCAAACGGTCCCGTGATGCAGAAAAAAACAGCTGGATGGGAACCGTGCGTTGAGATGCTTTATCCGCGGGACGGCGTCCTGTGTGGTCAGAGCCTGATGGCCCTGAAATGCACTGATGGCAATCATCTGACGTCCCACCTGCGCACTACCTATCGTTCTCGCAAGCCATCCAATGCAGTTAACATGCCGGAATTTCATTTTGGGGATCATCGCATTGAGATTTTGAAAGCTGAACAAGGTAAATTTTATGAACAATACGAGTCAGCGGTGGCCCGTTACTGTGAGGCGGCACCGAGTAAATTAGGGCATCACTAATAAAGGATCCAAAGGAGGCTCAAGTATGGGCATCATTCGCACATGTAGGCTCGGCCCTGACCAAGTCAAATCCATGCGGGCTGCTCTTGATCTTTTCGGTCGTGAGTTCGGAGACGTAGCCACCTACTCCCAACATCAGCCGGACTCCGATTACCTCGGGAACTTGCTCCGTAGTAAGACATTCATCGCGCTTGCTGCCTTCGACCAAGAAGCGGTTGTTGGCGCTCTCGCGGCTTACGTTCTGCCCAGGTTTGAGCAGCCGCGTAGTGAGATCTATATCTATGATCTCGCAGTCTCCGGCGAGCACCGGAGGCAGGGCATTGCCACCGCGCTCATCAATCTCCTCAAGCATGAGGCCAACGCGCTTGGTGCTTATGTGATCTACGTGCAAGCAGATTACGGTGACGATCCCGCAGTGGCTCTCTATACAAAGTTGGGCATACGGGAAGAAGTGATGCACTTTGATATCGACCCAAGTACCGCCACCTAATAATACTAGTAGCGGCCGCTGCAGCCC1347 bpTo be used as a template for the creation of gene cassettes with antibiotic resistance and phenotypic markers
LacI-lacO-pLac-attI GGGAATTCAAATCTAGAGACACCATCGAATGGTGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGAAGGAGGTGAATATGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTCAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTAATAAGCGCAAAAAAAAAACCCCGCCCCTGACAGGGCGGGGTTTTTTTTCAACGCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAGGCAAAACTAGTAAACTGCAGGG1403 bpTo be used as an AttI site with a Lac operon to allow controllable expression

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