Team:USyd-Australia/Notebook/gBlock

From 2014.igem.org

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gBlocks

All gBlocks were supplied by IDT.

gBlocks are DNA chunks up to 2kb made from a sequence template.

NameSequence (5'-3')SizePurpose
IntI1CCGTTTTTTTGGATGGAGTGAAACGATGGCGATTGCAATAGCTAGCTAAAGGAGGATTCATATGAAAACCGCCACTGCGCCGTTACCACCGCTGCGTTCGGTCAAGGTTCTGGACCAGTTGCGTGAGCGCATACGCTACTTGCATTACTCCCTCCGAACCGAACAGGCTTACGTTAACTGGGTTCGTGCCTTCATCCGTTTCCACGGTGTGCGTCACCCGGCAACCTTGGGCAGCAGCGAAGTCGAGGCATTTCTGTCCTGGCTGGCGAACGAGCGCAAGGTTTCGGTCTCCACGCATCGTCAGGCATTGGCGGCCTTGCTGTTCTTCTACGGCAAGGTGCTGTGCACGGATCTGCCCTGGCTTCAGGAGATCGGAAGACCTCGGCCGTCGCGGCGCTTGCCGGTGGTGCTGACCCCGGATGAAGTGGTTCGCATCCTCGGTTTTCTGGAAGGCGAGCATCGTTTGTTCGCCCAGCTTCTGTATGGAACGGGCATGCGGATCAGTGAGGGTTTGCAACTGCGGGTCAAGGATCTGGATTTCGATCACGGCACGATCATCGTGCGGGAGGGCAAGGGCTCCAAGGATCGGGCCTTGATGTTACCCGAGAGCTTGGCACCCAGCCTGCGCGAGCAGCTGTCGCGTGCACGGGCATGGTGGCTGAAGGACCAGGCCGAGGGCCGCAGCGGCGTTGCGCTTCCCGACGCCCTTGAGCGGAAGTATCCGCGCGCCGGGCATTCCTGGCCGTGGTTCTGGGTTTTTGCGCAGCACACGCATTCGACCGATCCACGGAGCGGTGTCGTGCGTCGCCATCACATGTATGACCAGACCTTTCAGCGCGCCTTCAAACGTGCCGTAGAACAAGCAGGCATCACGAAGCCCGCCACACCGCACACCCTCCGCCACTCGTTCGCGACGGCCTTGCTCCGCAGCGGTTACGACATTCGAACCGTGCAGGATCTGCTCGGCCATTCCGACGTCTCTACGACGATGATTTACACGCATGTGCTGAAAGTTGGCGGTGCCGGAGTGCGCTCACCGCTTGATGCGCTGCCGCCCCTCACTTCGGAGAGGTAGTACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAGGGCAAGGTGTCA1121 bpTo be inserted into SamR construct as part of the controllable integrase expression system
AttC-aeBlue-aacC1 TTTGAATTCGCGGCCGCTTCTAGAGTACCGAGGGACAGACTACCAACTCACAGCTAGCGCCTAACAATTCGTCCAAGCCGACGCCGCTTCGCGGCGCGGCTTAACTCAGGTGTTAGGCAAAGGAGGTTATTGATATGGCTTCACTGGTTAAAAAAGACATGTGCATCAAAATGACGATGGAAGGAACAGTAAACGGTCACCATTTCAAGTGTGTAGGAGAAGGCGAAGGCAAACCATTTGAAGGGACCCAGGTGGAAAAGATACGCATCACTGAAGGTGGGCCCTTACCATTTGCGTATGATATTTTGGCCCCTTGTTGCATGTATGGCAGTAAAACCTTCATTAAGCATGTGTCGGGTATTCCGGATTACTTTAAGGAGTCTTTTCCTGAGGGCTTTACCTGGGAAAGAACACAAATCTTCGAGGATGGCGGCTATCTCACCATACACCAGGACACGAGCCTTCAGGGTAATAATTTTATTTTCAAAGTTAATGTCATCGGTGCCAACTTCCCTGCAAACGGTCCCGTGATGCAGAAAAAAACAGCTGGATGGGAACCGTGCGTTGAGATGCTTTATCCGCGGGACGGCGTCCTGTGTGGTCAGAGCCTGATGGCCCTGAAATGCACTGATGGCAATCATCTGACGTCCCACCTGCGCACTACCTATCGTTCTCGCAAGCCATCCAATGCAGTTAACATGCCGGAATTTCATTTTGGGGATCATCGCATTGAGATTTTGAAAGCTGAACAAGGTAAATTTTATGAACAATACGAGTCAGCGGTGGCCCGTTACTGTGAGGCGGCACCGAGTAAATTAGGGCATCACTAATAAAGGATCCAAAGGAGGCTCAAGTATGGGCATCATTCGCACATGTAGGCTCGGCCCTGACCAAGTCAAATCCATGCGGGCTGCTCTTGATCTTTTCGGTCGTGAGTTCGGAGACGTAGCCACCTACTCCCAACATCAGCCGGACTCCGATTACCTCGGGAACTTGCTCCGTAGTAAGACATTCATCGCGCTTGCTGCCTTCGACCAAGAAGCGGTTGTTGGCGCTCTCGCGGCTTACGTTCTGCCCAGGTTTGAGCAGCCGCGTAGTGAGATCTATATCTATGATCTCGCAGTCTCCGGCGAGCACCGGAGGCAGGGCATTGCCACCGCGCTCATCAATCTCCTCAAGCATGAGGCCAACGCGCTTGGTGCTTATGTGATCTACGTGCAAGCAGATTACGGTGACGATCCCGCAGTGGCTCTCTATACAAAGTTGGGCATACGGGAAGAAGTGATGCACTTTGATATCGACCCAAGTACCGCCACCTAATAATACTAGTAGCGGCCGCTGCAGCCC1347 bpTo be used as a template for the creation of gene cassettes with antibiotic resistance and phenotypic markers
LacI-lacO-pLac-attI GGGAATTCAAATCTAGAGACACCATCGAATGGTGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGAAGGAGGTGAATATGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTCAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTAATAAGCGCAAAAAAAAAACCCCGCCCCTGACAGGGCGGGGTTTTTTTTCAACGCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAGGCAAAACTAGTAAACTGCAGGG1403 bpTo be used as an AttI site with a Lac operon to allow controllable expression

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