Team:USyd-Australia/Notebook/Primers

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Primers


Name Sequence (5'-3') Theoretical Tm Purpose
iGEM10 AGCTTTCGCTAAGGATGATTTCTGGA 58.2C Screening and sequencing
iGEM16 GAGTGCCACCTGACGTCTAAGAAACC 60.9C For BioBrick sequencing, amplification by PCR, or junction PCR. Alternative to iGEM VF2 primer
iGEM25 CCCTGATTCTGTGGATAACCGTATTACCG 60.0C For BioBrick sequencing, amplification by PCR, or junction PCR. Alternative to iGEM VR primer
iGEM1401 GGGAAGCTTTCTTAGACGTCAGGTGGCACTTTTCGG 66.3C pBBR replicon
iGEM1402 TTTGGATCCTATGCTGCTGGCTACCCTGTGGAAC 66.5C pBBR replicon
iGEM1403 GTTCCACAGGGTAGCCAGCAGCATAGGATCCAAAGAGTGCCACCTGACGTCTAAGAAACC 71.2C primers for pSB prefix/suffix
iGEM1404 CCGAAAAGTGCCACCTGACGTCTAAGAAAGCTTCCCCCTGATTCTGTGGATAACCGTATTACCG 70.3 primers for pSB prefix/suffix
iGEM1405 GAGGTCGGACAGTATATTGAAACGTCAGGAGTCTAGATACCGAGGGACAGACTACCAACTCACA primers for gene cassette circularisation (attC-aeBlue-aacC1)
iGEM1406 GTATCTAGACTCCTGACGTTTCAATATACTGTCCGACCTCTTATTAGGTGGCGGTACTTGGGTCG primers for gene cassette circularisation (attC-aeBlue-aacC1)
iGEM1407 CTATATCTATGATCTCGCAGTCTCC 53.8C PCR primers over the junction between aacC1 (G block) and pSB1C3 vector, for validation of successful G block insertion by PCR screening
iGEM1408 GCCTTTTGCTCACATGTTCTTTC 55.2C PCR primers over the junction between aacC1 (G block) and pSB1C3 vector, for validation of successful G block insertion by PCR screening
iGEM1409 GTTTTTTTGGATGGAGTGAAACGATGGCGATTG 61.7C Amplification of iGEM-IntI1 gBlock forward primer
iGEM1410 TGACACCTTGCCCTTTTTTGCCG 60.9C Amplification of iGEM-IntI1 gBlock reverse primer
iGEM1411 GTTTTTTTGGATGGAGTGAAACGATGGCGATTG 61.7C Gblock primers for IntI1 in pSAM-R
iGEM1412 TGACACCTTGCCCTTTTTTG 53.9C Gblock primers for IntI1 in pSAM-R
iGEM1413 GACATTGCCGTCACTGCGTC 59.1C Junction primers for IntI1 in pSAM-R
iGEM1414 TGGTCCAGAACCTTGACCGAAC 59.2C Junction primers for IntI1 in pSAM-R
iGEM1415 AACCGAGGATGCGAACCACTTC 59.7C Junction primers for IntI1 in pSAM-R
iGEM1416 CCTTCAAACGTGCCGTAGAACAAGC 60.4C Junction primers for IntI1 in pSAM-R
iGEM1417 AAATACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAG 67.6C Linearisation of pSB1C3, containing full BioBrick prefix and suffix
iGEM1418 AAACTCTAGAAGCGGCCGCGAATTCCAGAAATCATCCTTAGC 66.9C Linearisation of pSB1C3, containing full BioBrick prefix and suffix
iGEM1419 TTTGAATTCGCGGCCGCTTCTAGAGTACCGAGGGACAGAC 68.9C Amplification of aeBlue gBlock. Forward primer.
iGEM1420 GGGCTGCAGCGGCCGCTACTAGTATTATTAGGTGG 67.4C Amplification of aeBlue gBlock. Reverse primer.
iGEM1421 GGGAATTCAAATCTAGAGACACCATCG 57.1C Amplification of LacI-Plac-AttI gBlock. Forward primer
iGEM1422 CCTGCAGTTTACTAGTTTTGCCTAACTTTGTTTTAG 59.4C Amplification of LacI-Plac-AttI gBlock. Reverse primer
iGEM1423 CAGGCTTTACACTTTATGCTTCCG 56.3C lacI-Plac-attI RHJ-F right hand junction forward primer (use with iGEM25)
iGEM1424 AATGTAATTCAGCTCCGCCATC 55.5C lacI-Plac-attI LHJ-R left hand junction reverse primer (use with iGEM16)
iGEM1425 AAATCTAGATGGCGCGGCTTAACTCAGGTGTTAGGCTTAGGAGGCTCAAGTATGGGCAT 70.7C For making aacC1 gene cassettes from Tn1696. Use with iGEM1426.
iGEM1426 AAAACTAGTGGCGTCGGCTTGGACGAATTGTTAGGCTTAGGTGGCGGTACTTGGGTC 71.4C For making aacC1 gene cassettes from Tn1696. Use with iGEM1425.
iGEM1427 TTTTCTAGAGTTTGATGTTATGGAGCAGCAACG 60.2C For making aacC1 gene cassettes from Tn1696. Use with iGEM1428. Alternative to iGEM1424.
iGEM1428 TTTACTAGTGCAAAAAGGCAGCAATTATGAGCC 60.9C For making aacC1 gene cassettes from Tn1696. Use with iGEM1427. Alternative to iGEM1426.
NVC71 CTAAGAATCCATAGTCCAACTCC 52.4C aadB gene, rvs primer for junction screen
NVC92b CACGCAAGACCTCAACCTTTTCC 58.6C aadB gene, rvs primer for junction screen
NVC158 GATACCTTGTGCGGCTATGTCTG 57.6C pUS41/44, left of cloning site
NVC159 GCCGCCTTGGGCCGGGTGATGTC 69.2C pUS41/44, right of cloning site

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