Team:USyd-Australia/Notebook
From 2014.igem.org
(Difference between revisions)
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</tr> | </tr> | ||
+ | <tr> | ||
+ | <td width="10%" valign="top"> 15/5 | ||
+ | </td> | ||
+ | <td width="10%" valign="top">Thursday</td> | ||
+ | <td width="70%" valign="top">Preparation of common solutions:</br> | ||
+ | <ul> | ||
+ | <li>LB | ||
+ | <li>Stock Streptomycin (Sm) 200mg/mL, Chloramphenicol (Cm) 25 (mg/mL) | ||
+ | <li>Sm200 and Cm25 plates | ||
+ | </ul> | ||
+ | </td> | ||
+ | <td valign="top" >Callum and Jeanne</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td width="10%" valign="top"> 16/5 | ||
+ | </td> | ||
+ | <td width="10%" valign="top">Friday</td> | ||
+ | <td width="70%" valign="top">Preparation of common solutions:</br> | ||
+ | <ul> | ||
+ | <li>Stock Kanamycin (Km) 50 (mg/ml) | ||
+ | <li>Km50 plates | ||
+ | </ul> | ||
+ | </td> | ||
+ | <td valign="top" >Abi and Tom</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td width="10%" valign="top"> 22/5 | ||
+ | </td> | ||
+ | <td width="10%" valign="top">Thursday</td> | ||
+ | <td width="70%" valign="top">Preparation of common solutions:</br> | ||
+ | <ul> | ||
+ | <li>2M NaOH | ||
+ | <li>Stock Trimethoprim (Tm) 50mg/mL | ||
+ | </ul> | ||
+ | Restriction digest: | ||
+ | <ul> | ||
+ | <li>pK18 digested with HindIII, EcoRI, BamHI | ||
+ | </ul> | ||
+ | Agarose Gel Electrophoresis: | ||
+ | <ul> | ||
+ | <li>pK18 digests and undigested DNA | ||
+ | <li>Post-stained with GelRed | ||
+ | <li>Results...? | ||
+ | </ul> | ||
+ | </td> | ||
+ | <td valign="top" >Callum and Jeanne</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td width="10%" valign="top"> 23/5 | ||
+ | </td> | ||
+ | <td width="10%" valign="top">Friday</td> | ||
+ | <td width="70%" valign="top">Growing up gene source <i>E. coli</i></br> | ||
+ | <ul> | ||
+ | <li>JM109 containing pK18, for gene ____, on LB-Km | ||
+ | <li>_____ containing R388, for gene ____, on LB-Tm | ||
+ | <li>JM109 containing pUS44, for Pc-AttI1, on LB-Sm | ||
+ | <li>Transform pUS41 by heat shock into Top10, grow on Sm (in hindsight, Top10 already has Sm resistance, so this was not valid) | ||
+ | </ul> | ||
+ | One Loopful of each culture from glycerol stocks was streaked onto appropriate selective media under aseptic conditions. All plates were incubated at 30 degrees over the weekend. | ||
+ | </td> | ||
+ | <td valign="top" >Abi and Tom</td> | ||
+ | </tr> | ||
</table> | </table> | ||
</html> | </html> |
Revision as of 09:15, 21 September 2014
Notebook
Date
Day
Activity
Who?
- LB
- Stock Streptomycin (Sm) 200mg/mL, Chloramphenicol (Cm) 25 (mg/mL)
- Sm200 and Cm25 plates
- Stock Kanamycin (Km) 50 (mg/ml)
- Km50 plates
- 2M NaOH
- Stock Trimethoprim (Tm) 50mg/mL
- pK18 digested with HindIII, EcoRI, BamHI
- pK18 digests and undigested DNA
- Post-stained with GelRed
- Results...?
- JM109 containing pK18, for gene ____, on LB-Km
- _____ containing R388, for gene ____, on LB-Tm
- JM109 containing pUS44, for Pc-AttI1, on LB-Sm
- Transform pUS41 by heat shock into Top10, grow on Sm (in hindsight, Top10 already has Sm resistance, so this was not valid)