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We constructed the plasmid with lactose operator and signal peptide, and transform this plasmid into E.coil. to test if this kill switch do work. We used gradient plate method in our experiment. As we can see in the picture, the concentration of IPTG in the left of the medium is 0.1% while the concentration on the right is zero. We can see colony only on the right of the medium, the kill switch is effective which illustrates that IPTG can induce the expression of signal peptide so as to inhibit the growth of the E.coil. Also, a decrease of OD is observed after we added IPTG to the liquid culture medium.
However ,we haven’t connect the LALF gene with signal peptide yet due to the time limit. We will construct the whole plasmid and transform it into E.coil. to see the sterilization effect of LALF in the future work.
