Team:USTC-China/safety

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           <a href="#panel1">Safety</a>
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      <h1>Safety</h1>
 
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                <h2 id="safetytraininginourteam">Safety Training in our team</h2>
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    <h2 id="killswitchinecoli">Kill Switch in <em>E.coli</em></h2>
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    <p><b>Q1:Would any of your project ideas raise safety issues in terms of: researcher safety, public safety, or environmental safety?</b></p>
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    <p>This year our safety work is based on <em>E.coli</em>, since some of our work is on light sensing and imaging system in <em>E.coli</em>. To make a wider application in the future, the biosafety of <em>E.coli</em> containing these parts should be concerned.</p>
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        <p>A: All of our team members had safety training and our operation is according to the safety instructions. We use <i>E.coli</i> and <i>Caulobacter crescentus</i> in our lab and both of them belong to Risk 1 Group, so it's safe relatively as long as we follow the rules in our lab. The protein we expressed is also innocuous. Even if it's mishandled accidently, any negative effect upon researchers, public or environment would be neglectable.</p>
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    <p>LALF is </p>
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        <p><b>Q2:Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues?
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    <p>We designed the part based on K541545. We found that R0010 might keep express genes below without regulation.Reason may be that Lacl expressed by E.coil is limited. So we inserted parts that express Lacl and constitutive promoter before the old one to inhibite pLac. <br />
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        <p>A: The fluorescent proteins and the chromoprotein are widely used, so none of the new biobrick parts that we made this year raise any safety issue.</p>
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    <h3 id="antilpsfactorlalfregulatedbyiptg">Anti-LPS factor(LALF) regulated by IPTG</h3>
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          <p><b>Q3:Is there a local biosafety group, committee, or review board at your institution? If yes, what does your local biosafety group think about your project?</b></p>
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    <p>This part is a cell-kill system. Firstly,constitutive promoter could express enough Lacl that is useful for downstream. Secondly, the reporter in the middle of our part wouldn't show signal nor the cell be killed by the part behind unless IPTG or lactose added into the culture medium. No IPTG or lactose: GFP don't express ,G- won't be killed. IPTG or lactose exsisting : GFPs express ,G- will be dead. </p>
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          <p>A: Professor Zhi-Gang Tian is responsible for biological safety at our institution.
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Yes,we have been discussing the possible risks like gene pollution and the healthy of people in the lab. Professor Tian had suggested us that we make a duty report to supervise the regulation in our lab to prevent the possible risks and it works well.<p/>
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    <p>G-: Gram negative bacteria <br />
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          <p><b>Q4:Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering?</b></p>
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          <p>A: The resistance gene of our E.coli may cause genetic pollution. As antibiotic-resistance genes are commonly used as markers during plasmid construction, there is therefore major concern that their presence in environment released GMMs could contribute to the generation of antibiotic-resistant 'superbugs'. So we think maybe we could use auxotrophic bacteria for our screening in the future work.<p/>
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Latest revision as of 02:45, 18 October 2014


Q1:Would any of your project ideas raise safety issues in terms of: researcher safety, public safety, or environmental safety?


A: All of our team members had safety training and our operation is according to the safety instructions. We use E.coli and Caulobacter crescentus in our lab and both of them belong to Risk 1 Group, so it's safe relatively as long as we follow the rules in our lab. The protein we expressed is also innocuous. Even if it's mishandled accidently, any negative effect upon researchers, public or environment would be neglectable.



Q2:Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues?


A: The fluorescent proteins and the chromoprotein are widely used, so none of the new biobrick parts that we made this year raise any safety issue.



Q3:Is there a local biosafety group, committee, or review board at your institution? If yes, what does your local biosafety group think about your project?


A: Professor Zhi-Gang Tian is responsible for biological safety at our institution. Yes,we have been discussing the possible risks like gene pollution and the healthy of people in the lab. Professor Tian had suggested us that we make a duty report to supervise the regulation in our lab to prevent the possible risks and it works well.



Q4:Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering?


A: The resistance gene of our E.coli may cause genetic pollution. As antibiotic-resistance genes are commonly used as markers during plasmid construction, there is therefore major concern that their presence in environment released GMMs could contribute to the generation of antibiotic-resistant 'superbugs'. So we think maybe we could use auxotrophic bacteria for our screening in the future work.