Team:UNIK Copenhagen/Quenchibodies

From 2014.igem.org

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<p align="justify">The Ultra Quenchibody Project focuses on the construction of a fluorescent antibody fragment (FAB) covalently linked to a fluorophore at the N-terminal site.  Light emission from the attached fluorophore is quenched by tryptophan residues in the FAB fragment. Upon binding of the antigen, the FAB fragment undergoes structural changes, which displace the fluorophore away from the tryptophan residues eliminating the quenching effect. In this way binding of the antigen results in fluorescence. <br><br>
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<p align="justify">The Ultra Quenchibody Project focuses on the construction of a <abbr title="Fragment antigen binding">FAB</abbr> covalently linked to a fluorophore at the N-terminal site.  Light emission from the attached fluorophore is quenched by tryptophan residues in the FAB fragment.<img src="https://static.igem.org/mediawiki/2014/7/76/Team_UNIK_Copenhagen_Quenchibody_illustration.png" class="right"> Upon binding of the antigen, the FAB fragment undergoes structural changes, which displace the fluorophore away from the tryptophan residues eliminating the quenching effect. In this way binding of the antigen results in fluorescence. <br><br>
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Estradiol and progesterone are both environmentally damaging hormones, which in excess are environmentally hazardous due to interference with the reproductive systems of animals, and can lead to various types of cancer including breast and uterus cancer. Within the Quenchibody project we will construct two fluorescent detection FAB fragments. The FAB fragments will be derived from antibodies binding to the sex hormones estradiol and progesterone. These products will thus allow for fast and easy ne step detection method of the levels of both hormones.</p>
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Estradiol and progesterone are both environmentally damaging hormones, which in excess are environmentally hazardous due to interference with the reproductive systems of animals, and can lead to various types of cancer including breast and uterus cancer. Within the Quenchibody project we will construct two fluorescent detection FAB fragments. The FAB fragments will be derived from antibodies binding to the sex hormones estradiol and progesterone. These products will thus allow for fast and easy step detection method of the levels of both hormones.</p>
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<p><br>Touch the lego bricks to see what sequences the gene consist of and click on the sequences to read more about their function.</p>
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<div class="subject">
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<h3>GENE CONSTRUCTS</h3>
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<div class="description">
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<p><br><b>Touch</b> the lego bricks to see what sequences the gene consist of and <b>click</b> on the sequences to read more about their function. Note that the information box will be shown under the pictures.</p>
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<p>Gene construct 1:</p>
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<p>Gene construct 1: LightChain-FLAG</p>
<img src="https://static.igem.org/mediawiki/2014/7/74/Team_UNIK_Copenhagen_Quenchibodies_construct1.PNG"  usemap="#MapGENE1" border="0">
<img src="https://static.igem.org/mediawiki/2014/7/74/Team_UNIK_Copenhagen_Quenchibodies_construct1.PNG"  usemap="#MapGENE1" border="0">
<map name="MapGENE1">
<map name="MapGENE1">
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   <area shape="rect" coords="21,20,160,85" title="Viable part of the light chain" type="button" onclick="lightVFunction();">
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   <area shape="rect" coords="2,2,112,50" title="Viable part of the light chain" type="button" onclick="lightVFunction();">
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   <area shape="rect" coords="171,20,311,85" title="Conserved part of the light chain" type="button" onclick="lightCFunction();">
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   <area shape="rect" coords="114,2,224,50" title="Conserved part of the light chain" type="button" onclick="lightCFunction();">
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   <area shape="rect" coords="314,20,380,85" title="FLAG tag" type="button" onclick="flagtFunction();">
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   <area shape="rect" coords="225,2,279,50" title="FLAG-tag" type="button" onclick="flagtFunction();">
</map>
</map>
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                        <p>Gene construct 2: ProXtag-HeavyChain-HIStag</p>
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<img src="https://static.igem.org/mediawiki/2014/4/4c/Team_UNIK_Copenhagen_Quenchibodies_construct_2.png" usemap="#MapGENE2" border="0">
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<map name="MapGENE2">
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  <area shape="rect" coords="2,2,57,50" title="ProX-tag" type="button" onclick="proxtFunction();">
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  <area shape="rect" coords="58,2,169,50" title="Viable part of the heavy chain" type="button" onclick="heavyVFunction();">
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  <area shape="rect" coords="170,2,280,50" title="Conserved part of the heavy chain" type="button" onclick="heavyCFunction();">
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  <area shape="rect" coords="281,2,336,50" title="His-tag" type="button" onclick="histFunction();">
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</map>
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<br>
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<br>
<table class="sequence_description" id="about_gene"></table></div>
<table class="sequence_description" id="about_gene"></table></div>
<script>
<script>
function lightVFunction() {
function lightVFunction() {
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     document.getElementById("about_gene").innerHTML="<p>This sequence codes for the viable part of the light chain in the FAB fragment</p>";
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     document.getElementById("about_gene").innerHTML="<p>This sequence encodes the viable part of the light chain in the FAB fragment</p>";
}
}
function lightCFunction() {
function lightCFunction() {
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     document.getElementById("about_gene").innerHTML="<p>This sequence codes for the conserved part of the light in the FAB fragment.</p>";
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     document.getElementById("about_gene").innerHTML="<p>This sequence encodes the conserved part of the light in the FAB fragment.</p>";
}
}
function flagtFunction() {
function flagtFunction() {
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     document.getElementById("about_gene").innerHTML="<p>This seguence codes for a FLAG tag.</p>";
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     document.getElementById("about_gene").innerHTML="<p>This seguence encodes a FLAG-tag. FLAG-tag is a polypeptide and we have added it to purify our light chain.</p>";
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}
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function proxtFunction() {
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    document.getElementById("about_gene").innerHTML="<p>This sequence encodes a ProX-tag.</p>";
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}
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function heavyVFunction() {
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    document.getElementById("about_gene").innerHTML="<p>This sequence encodes the viable part of the heavy chain in the FAB fragment</p>";
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}
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function heavyCFunction() {
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    document.getElementById("about_gene").innerHTML="<p>This sequence encodes the conserved part of the heavy in the FAB fragment.</p>";
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}
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function histFunction() {
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    document.getElementById("about_gene").innerHTML="<p>This sequence encodes a His-tag.</p>";
}
}
</script>
</script>
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<p>Team UNIK Copenhagen <br> <b>e-mail:</b> igem.cph14@gmail.com<p><br>
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Latest revision as of 18:55, 11 September 2014




QUENCHIBODIES

The Ultra Quenchibody Project focuses on the construction of a FAB covalently linked to a fluorophore at the N-terminal site. Light emission from the attached fluorophore is quenched by tryptophan residues in the FAB fragment. Upon binding of the antigen, the FAB fragment undergoes structural changes, which displace the fluorophore away from the tryptophan residues eliminating the quenching effect. In this way binding of the antigen results in fluorescence.

Estradiol and progesterone are both environmentally damaging hormones, which in excess are environmentally hazardous due to interference with the reproductive systems of animals, and can lead to various types of cancer including breast and uterus cancer. Within the Quenchibody project we will construct two fluorescent detection FAB fragments. The FAB fragments will be derived from antibodies binding to the sex hormones estradiol and progesterone. These products will thus allow for fast and easy step detection method of the levels of both hormones.

GENE CONSTRUCTS


Touch the lego bricks to see what sequences the gene consist of and click on the sequences to read more about their function. Note that the information box will be shown under the pictures.

Gene construct 1: LightChain-FLAG

Gene construct 2: ProXtag-HeavyChain-HIStag





Retrieved from "http://2014.igem.org/Team:UNIK_Copenhagen/Quenchibodies"