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Team:UNIK Copenhagen/Project
From 2014.igem.org
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<p>Demands for fast, cheap and environmentally responsible detection methods are rising. To accomondate this, the iGEM Team of the University of Copenhagen aims at creating a bio-engineered diagnostic tool based on antibodies with novel flourescent markers for a fast, non-toxic and easy-to-use detection of pathogens. | <p>Demands for fast, cheap and environmentally responsible detection methods are rising. To accomondate this, the iGEM Team of the University of Copenhagen aims at creating a bio-engineered diagnostic tool based on antibodies with novel flourescent markers for a fast, non-toxic and easy-to-use detection of pathogens. | ||
| - | To achieve this goal we take two different approaches. In the first approach a tripartite split GFP system is employed. Click <a href="https://2014.igem.org/Team:UNIK_Copenhagen/Tripartite_split_GFP" ><font color="0B6138"><b>here</b></font></a> to read more about the tripartite split GFP project. The second approach is the construction of a flourescent antibody fragment (FAB) by covalently linking a fluorophore o the N-terminus. <br><br> | + | To achieve this goal we take two different approaches. In the first approach a tripartite split GFP system is employed. Click <a href="https://2014.igem.org/Team:UNIK_Copenhagen/Tripartite_split_GFP" ><font color="0B6138"><b>here</b></font></a> to read more about the tripartite split GFP project. The second approach is the construction of a flourescent antibody fragment (FAB) by covalently linking a fluorophore o the N-terminus. Click <a href="https://2014.igem.org/Team:UNIK_Copenhagen/Quenchibodies" ><font color="0B6138"><b>here</b></font></a> to read more about the quenchibody project. <br><br> |
We aim at developing a non-toxic detection tool that is both environmentally responsible and effective without relying on trained personnel. By using bacteria and yeast as our production platform, we aim at reducing cost, chemicals and waste in production. <br><br> | We aim at developing a non-toxic detection tool that is both environmentally responsible and effective without relying on trained personnel. By using bacteria and yeast as our production platform, we aim at reducing cost, chemicals and waste in production. <br><br> | ||
Our project will add new parts useful for synthetic biology. To better understand the dynamics and thus predict functions we have modelled the complexes used in this study and other antibodies. We have chosen three distinct antibodies for this project but the conserved nature of antibodies shown in our modelling suggest that the method of creation fluorescent detectors we have engineered will possibly be applicable to other antibodies and systems with the same physiochemical dynamics. | Our project will add new parts useful for synthetic biology. To better understand the dynamics and thus predict functions we have modelled the complexes used in this study and other antibodies. We have chosen three distinct antibodies for this project but the conserved nature of antibodies shown in our modelling suggest that the method of creation fluorescent detectors we have engineered will possibly be applicable to other antibodies and systems with the same physiochemical dynamics. | ||
Revision as of 13:08, 28 July 2014
BIO SENSORS WITH NOVEL FLUORESCENT MARKERS
Demands for fast, cheap and environmentally responsible detection methods are rising. To accomondate this, the iGEM Team of the University of Copenhagen aims at creating a bio-engineered diagnostic tool based on antibodies with novel flourescent markers for a fast, non-toxic and easy-to-use detection of pathogens.
To achieve this goal we take two different approaches. In the first approach a tripartite split GFP system is employed. Click here to read more about the tripartite split GFP project. The second approach is the construction of a flourescent antibody fragment (FAB) by covalently linking a fluorophore o the N-terminus. Click here to read more about the quenchibody project.
We aim at developing a non-toxic detection tool that is both environmentally responsible and effective without relying on trained personnel. By using bacteria and yeast as our production platform, we aim at reducing cost, chemicals and waste in production.
Our project will add new parts useful for synthetic biology. To better understand the dynamics and thus predict functions we have modelled the complexes used in this study and other antibodies. We have chosen three distinct antibodies for this project but the conserved nature of antibodies shown in our modelling suggest that the method of creation fluorescent detectors we have engineered will possibly be applicable to other antibodies and systems with the same physiochemical dynamics.
