Team:UMaryland/project/biobricks

From 2014.igem.org

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<p style="text-align: center;"><span style="color: #282828; font-family: arial, sans-serif; font-size: 20px; font-weight: bold; line-height: 19.5px; text-align: start;">Bovine Galectin-1</span></p>
<p style="text-align: center;"><span style="color: #282828; font-family: arial, sans-serif; font-size: 20px; font-weight: bold; line-height: 19.5px; text-align: start;">Bovine Galectin-1</span></p>
<p style="margin: 0.4em 0px 0.5em; line-height: 19.5px; color: #282828; font-family: arial, sans-serif; font-size: 13px;">Soluble beta-galactoside binding protein (galectin) originally from Bos taurus. It is believed to expressed both intracellularly and extracellularly, where they serve to binding various types of beta-galactosides and initialize signal cascades. It is believed to be a dimer in its active form, as is true with most mammalian galectins.</p>
<p style="margin: 0.4em 0px 0.5em; line-height: 19.5px; color: #282828; font-family: arial, sans-serif; font-size: 13px;">Soluble beta-galactoside binding protein (galectin) originally from Bos taurus. It is believed to expressed both intracellularly and extracellularly, where they serve to binding various types of beta-galactosides and initialize signal cascades. It is believed to be a dimer in its active form, as is true with most mammalian galectins.</p>
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<p style="margin: 0.4em 0px 0.5em; line-height: 19.5px; color: #282828; font-family: arial, sans-serif; font-size: 13px;">UMaryland 2014 is interested in utilizing this part to investigate whether E. coli can recognize and bind to surface carbohydrates on a marine pathogen. Other uses of this part lie in the recognition of various carbohydrate ligands and potential activation of signal transduction pathways.</p>
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<p style="margin: 0.4em 0px 0.5em; line-height: 19.5px; color: #282828; font-family: arial, sans-serif; font-size: 13px;">UMaryland 2014 is interested in utilizing this part to investigate whether E. coli can recognize and bind to surface carbohydrates on a marine pathogen. Other uses of this part lie in the recognition of various carbohydrate ligands and potential activation of signal transduction pathways.</p>
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<p style="margin: 0.4em 0px 0.5em; text-align: center;"><strong><span style="color: #282828; font-family: arial, sans-serif; font-size: small;"><span style="line-height: 19.5px;">Part:BBa_K1489005: </span></span><span style="text-align: left; line-height: 19.5px;"><span style="color: #282828; font-family: arial, sans-serif; font-size: small;">Crasseoterra virginica Galectin 1 (CvGal1)</span></span></strong></p>
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<p style="margin: 0.4em 0px 0.5em; line-height: 19.5px; color: #282828; font-family: arial, sans-serif; font-size: 13px;"> </p>
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<p style="margin: 0.4em 0px 0.5em; line-height: 19.5px; color: #282828; font-family: arial, sans-serif; font-size: 13px;"><img src="http://parts.igem.org/wiki/images/0/01/UM_PSB1C3_-_BBa_K1489005.png" border="0" width="515" height="335" style="color: #141823; font-family: Helvetica, Arial, 'lucida grande', tahoma, verdana, arial, sans-serif; font-size: small; line-height: 17.9400005340576px; text-align: center; white-space: pre-wrap; cursor: se-resize !important; display: block; border: 0px; margin-left: auto; margin-right: auto;" /></p>
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<p style="margin: 0.4em 0px 0.5em; line-height: 19.5px; color: #282828; font-family: arial, sans-serif; font-size: 13px; text-align: center;"><span style="font-size: 20px; font-weight: bold; text-align: start;">Crasseoterra virginica Galectin 1 (CvGal1)</span></p>
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<p style="margin: 0.4em 0px 0.5em; line-height: 19.5px; color: #282828; font-family: arial, sans-serif; font-size: 13px;"><span style="font-size: 13px;">His-tagged soluble beta-galactoside binding protein (galectin) originally from Crasseoterra virginica. CvGal1 is believed to expressed both intracellularly and extracellularly, where they serve to binding various types of n-acetylgalactosamines and initialize signal cascades. It is also believed to be a tetramer in its active form, as is true with most invertebrate galectins.</span></p>
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<p style="margin: 0.4em 0px 0.5em; line-height: 19.5px; color: #282828; font-family: arial, sans-serif; font-size: 13px;"><span style="font-size: 13px;"> </span></p>
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<p style="margin: 0.4em 0px 0.5em; text-align: center;"><span style="text-decoration: underline;"><strong><span style="color: #282828; font-family: arial, sans-serif; font-size: small; text-decoration: underline;"><span style="line-height: 19.5px;">Part:BBa_K1489006: </span></span><span style="color: #282828; font-family: arial, sans-serif; font-size: small;"><span style="line-height: 19.5px;">OmpA-CvGal1</span></span></strong></span></p>
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<p style="margin: 0.4em 0px 0.5em; text-align: center;"><span style="text-decoration: underline;"><strong><span style="color: #282828; font-family: arial, sans-serif; font-size: small;"><span style="line-height: 19.5px;"> </span></span></strong></span></p>
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<p style="margin: 0.4em 0px 0.5em; line-height: 19.5px; color: #282828; font-family: arial, sans-serif; font-size: 13px;"><img src="http://parts.igem.org/wiki/images/2/28/UM_PSB1C3_-_BBa_K1489006.png" border="0" width="515" height="335" style="font-size: small; color: #141823; font-family: Helvetica, Arial, 'lucida grande', tahoma, verdana, arial, sans-serif; line-height: 17.9400005340576px; text-align: center; white-space: pre-wrap; display: block; cursor: se-resize !important; border: 0px; margin-left: auto; margin-right: auto;" /></p>
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<p style="margin: 0.4em 0px 0.5em; line-height: 19.5px; color: #282828; font-family: arial, sans-serif; font-size: 13px; text-align: center;"><span style="font-size: 20px; font-weight: bold; text-align: start;">OmpA-CvGal1</span></p>
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<p style="margin: 0.4em 0px 0.5em; line-height: 19.5px; color: #282828; font-family: arial, sans-serif; font-size: 13px; text-align: center;"><span style="font-size: 20px; font-weight: bold; text-align: start;"> </span></p>
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<p style="margin: 0.4em 0px 0.5em; line-height: 19.5px; color: #282828; font-family: arial, sans-serif; font-size: 13px; text-align: left;"><span style="font-size: 13px;">CvGal1 fused to OmpA in order to anchor it in the membrane.</span></p>
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Latest revision as of 20:40, 17 October 2014

Parts (BioBricks)

About Umaryland

UMaryland 2014 is the inaugural iGEM team of the University of Maryland, College Park. We are a combined effort of several departments and numerous faculty mentors. Although it is only our first year, we believe our hard work and dedication has paid off. We can't wait for this year's competition! GO TERPS!