Team:UMaryland/project/biobricks

From 2014.igem.org

(Difference between revisions)
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<p style="text-align: center;"><span style="color: #141823; font-family: Helvetica, Arial, 'lucida grande', tahoma, verdana, arial, sans-serif; font-size: 13px; line-height: 17.9400005340576px; white-space: pre-wrap;">A soluble his-tagged galectin from Bos taurus, expressed under Arabinose induction</span></p>
<p style="text-align: center;"><span style="color: #141823; font-family: Helvetica, Arial, 'lucida grande', tahoma, verdana, arial, sans-serif; font-size: 13px; line-height: 17.9400005340576px; white-space: pre-wrap;">A soluble his-tagged galectin from Bos taurus, expressed under Arabinose induction</span></p>
<p style="margin: 0.4em 0px 0.5em; line-height: 19.5px; color: #282828; font-family: arial, sans-serif; font-size: 13px; text-align: center;"><span style="font-size: 20px; font-weight: bold;">pBAD-RBS-Bovine Galectin-1-B0012 Terminator</span></p>
<p style="margin: 0.4em 0px 0.5em; line-height: 19.5px; color: #282828; font-family: arial, sans-serif; font-size: 13px; text-align: center;"><span style="font-size: 20px; font-weight: bold;">pBAD-RBS-Bovine Galectin-1-B0012 Terminator</span></p>
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<p style="margin: 0.4em 0px 0.5em; line-height: 19.5px; color: #282828; font-family: arial, sans-serif; font-size: 13px;">Soluble beta-galactoside binding protein (galectin) originally from Bos taurus. Believed to expressed both intracellularly and extracellularly, where they serve to binding various types of beta-galactosides and initialize signal cascades. Believed to be a dimer in its active form, as is true with most mammalian galectins.</p>
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<p style="margin: 0.4em 0px 0.5em; line-height: 19.5px; color: #282828; font-family: arial, sans-serif; font-size: 13px;">Soluble beta-galactoside binding protein (galectin) originally from Bos taurus. It is believed to expressed both intracellularly and extracellularly, where they serve to binding various types of beta-galactosides and initialize signal cascades. It is believed to be a dimer in its active form, as is true with most mammalian galectins.</p>
<p style="margin: 0.4em 0px 0.5em; line-height: 19.5px; color: #282828; font-family: arial, sans-serif; font-size: 13px;">This biobrick is under the pBAD promoter (BBa_K206000) with attached RBS (BBa_B0034), and utilizes an additional RNA polymerase terminator (BBa_B0012).</p>
<p style="margin: 0.4em 0px 0.5em; line-height: 19.5px; color: #282828; font-family: arial, sans-serif; font-size: 13px;">This biobrick is under the pBAD promoter (BBa_K206000) with attached RBS (BBa_B0034), and utilizes an additional RNA polymerase terminator (BBa_B0012).</p>
<p style="margin: 0.4em 0px 0.5em; line-height: 19.5px; color: #282828; font-family: arial, sans-serif; font-size: 13px;">UMaryland 2014 is interested in utilizing this part to investigate whether E. coli can recognize and bind to surface carbohydrates on a marine pathogen. Other uses of this part lie in the recognition of various carbohydrate ligands and potential activation of signal transduction pathways.</p>
<p style="margin: 0.4em 0px 0.5em; line-height: 19.5px; color: #282828; font-family: arial, sans-serif; font-size: 13px;">UMaryland 2014 is interested in utilizing this part to investigate whether E. coli can recognize and bind to surface carbohydrates on a marine pathogen. Other uses of this part lie in the recognition of various carbohydrate ligands and potential activation of signal transduction pathways.</p>
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<p style="text-align: center;"><span style="color: #141823; font-family: Helvetica, Arial, 'lucida grande', tahoma, verdana, arial, sans-serif; font-size: small;"><span style="line-height: 17.9400005340576px; white-space: pre-wrap;">This is the Bovine Galectin which should interact with beta-galactosides and intialize a signal cascase</span></span></p>
<p style="text-align: center;"><span style="color: #141823; font-family: Helvetica, Arial, 'lucida grande', tahoma, verdana, arial, sans-serif; font-size: small;"><span style="line-height: 17.9400005340576px; white-space: pre-wrap;">This is the Bovine Galectin which should interact with beta-galactosides and intialize a signal cascase</span></span></p>
<p style="text-align: center;"><span style="color: #282828; font-family: arial, sans-serif; font-size: 20px; font-weight: bold; line-height: 19.5px; text-align: start;">Bovine Galectin-1</span></p>
<p style="text-align: center;"><span style="color: #282828; font-family: arial, sans-serif; font-size: 20px; font-weight: bold; line-height: 19.5px; text-align: start;">Bovine Galectin-1</span></p>
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<p style="margin: 0.4em 0px 0.5em; line-height: 19.5px; color: #282828; font-family: arial, sans-serif; font-size: 13px;">Soluble beta-galactoside binding protein (galectin) originally from Bos taurus. Believed to expressed both intracellularly and extracellularly, where they serve to binding various types of beta-galactosides and initialize signal cascades. Believed to be a dimer in its active form, as is true with most mammalian galectins.</p>
+
<p style="margin: 0.4em 0px 0.5em; line-height: 19.5px; color: #282828; font-family: arial, sans-serif; font-size: 13px;">Soluble beta-galactoside binding protein (galectin) originally from Bos taurus. It is believed to expressed both intracellularly and extracellularly, where they serve to binding various types of beta-galactosides and initialize signal cascades. It is believed to be a dimer in its active form, as is true with most mammalian galectins.</p>
<p style="margin: 0.4em 0px 0.5em; line-height: 19.5px; color: #282828; font-family: arial, sans-serif; font-size: 13px;">UMaryland 2014 is interested in utilizing this part to investigate whether E. coli can recognize and bind to surface carbohydrates on a marine pathogen. Other uses of this part lie in the recognition of various carbohydrate ligands and potential activation of signal transduction pathways.</p>
<p style="margin: 0.4em 0px 0.5em; line-height: 19.5px; color: #282828; font-family: arial, sans-serif; font-size: 13px;">UMaryland 2014 is interested in utilizing this part to investigate whether E. coli can recognize and bind to surface carbohydrates on a marine pathogen. Other uses of this part lie in the recognition of various carbohydrate ligands and potential activation of signal transduction pathways.</p>
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Revision as of 22:05, 16 October 2014

Parts (BioBricks)

About Umaryland

UMaryland2014 is University of Maryland, College Parks, inaugural iGEM team. We are a combined effort of several departments and numerous faculty mentors. Although it is only our first year, believe our hard work and dedication has paid off. We can't wait for this years competition! GO TERPS!