Team:UMaryland/project/biobricks

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<li class="item101 level0 first"><span class="mymarg"><a class="yjanchor  first" href="/iGemWiki/"><span class="yjm_has_none"><span class="yjm_title">Homepage</span></span></a></span></li><li class="item104 level0"><span class="mymarg"><a class="yjanchor " href="https://2014.igem.org/Team:UMaryland/photos"><span class="yjm_has_none"><span class="yjm_title">Photos</span></span></a></span></li><li class="haschild item105 level0"><span class="child"><a class="yjanchor " href="https://2014.igem.org/Team:UMaryland/meetourteam"><span class="yjm_has_none"><span class="yjm_title">Team</span></span></a></span><ul class="subul_main level1"><li class="bl"></li><li class="tl"></li><li class="tr"></li><li class="item293 level1 first"><span class="mymarg"><a class="yjanchor  first" href="https://2014.igem.org/Team:UMaryland/meetourteam"><span class="yjm_has_none"><span class="yjm_title">Meet Our Team Members</span></span></a></span></li><li class="item116 level1"><span class="mymarg"><a 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class="item138 level1 first"><span class="mymarg"><a class="yjanchor  first" href="https://2014.igem.org/Team:UMaryland/project/overview"><span class="yjm_has_none"><span class="yjm_title">Overview</span></span></a></span></li><li class="item145 level1"><span class="mymarg"><a class="yjanchor " href="https://2014.igem.org/Team:UMaryland/project/background"><span class="yjm_has_none"><span class="yjm_title">Background</span></span></a></span></li><li id="current" class=" active item144 level1"><span class="mymarg"><a class="yjanchor  activepath " href="https://2014.igem.org/Team:UMaryland/project/biobricks "><span class="yjm_has_none"><span class="yjm_title">parts (BioBricks)</span></span></a></span></li><li class="item102 level1"><a class="yjanchor " href="https://2014.igem.org/Team:UMaryland/project/results" target="_blank" ><span class="yjm_has_none"><span class="yjm_title">Results</span></span></a></li><li class="item254 level1"><span class="mymarg"><a class="yjanchor " href="https://2014.igem.org/Team:UMaryland/project/futureapplications"><span class="yjm_has_none"><span class="yjm_title">future applications</span></span></a></span></li><li class="item301 level1 lilast"><span class="mymarg"><a class="yjanchor last" href="https://igem.org/Safety/Safety_Form?team_id=1489"><span class="yjm_has_none"><span class="yjm_title">Safety</span></span></a></span></li><li class="right"></li><li class="br"></li></ul></li><li class="haschild item234 level0"><span class="child"><a class="yjanchor " href="https://2014.igem.org/Team:UMaryland/notebook"><span class="yjm_has_none"><span class="yjm_title">Notebook</span></span></a></span><ul class="subul_main level1"><li class="bl"></li><li class="tl"></li><li class="tr"></li><li class="item303 level1 first lilast"><span class="mymarg"><a class="yjanchor  firstlast" href="https://2014.igem.org/Team:UMaryland/notebook"><span class="yjm_has_none"><span class="yjm_title">Wetlab Notebook</span></span></a></span></li><li class="right"></li><li class="br"></li></ul></li><li class="haschild item123 level0"><span class="child"><a class="yjanchor " href="https://2014.igem.org/Team:UMaryland/humanprac/overview"><span class="yjm_has_none"><span class="yjm_title">Human Practices</span></span></a></span><ul class="subul_main level1"><li class="bl"></li><li class="tl"></li><li class="tr"></li><li class="item106 level1 first"><span class="mymarg"><a class="yjanchor  first" href="https://2014.igem.org/Team:UMaryland/humanprac/overview"><span class="yjm_has_none"><span class="yjm_title">Overview | Mission Statement</span></span></a></span></li><li class="haschild item129 level1"><span class="child subparent"><a class="yjanchor  grouptitle" href="https://2014.igem.org/Team:UMaryland/humanprac/publicschool"><span class="yjm_has_none"><span class="yjm_title">Public School Outreach</span></span></a></span><ul class="subul_main level2"><li class="bl"></li><li class="tl"></li><li class="tr"></li><li class="item134 level2 first lilast"><span class="mymarg"><a class="yjanchor  firstlast" href="https://2014.igem.org/Team:UMaryland/humanprac/publicschool"><span class="yjm_has_none"><span class="yjm_title">HighSchool</span></span></a></span></li><li class="right"></li><li class="br"></li></ul></li><li class="item131 level1"><span class="mymarg"><a class="yjanchor " href="https://2014.igem.org/Team:UMaryland/humanprac/community"><span class="yjm_has_none"><span class="yjm_title">Community Interaction</span></span></a></span></li><li class="item132 level1 lilast"><span class="mymarg"><a class="yjanchor last" href="https://2014.igem.org/Team:UMaryland/humanprac/publicawareness"><span class="yjm_has_none"><span class="yjm_title">Public Awareness</span></span></a></span></li><li class="right"></li><li class="br"></li></ul></li></ul></div>
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<li class="item101 level0 first"><span class="mymarg"><a class="yjanchor  first" href="https://2014.igem.org/Team:UMaryland"><span class="yjm_has_none"><span class="yjm_title">Homepage</span></span></a></span></li><li class="item104 level0"><span class="mymarg"><a class="yjanchor " href="https://2014.igem.org/Team:UMaryland/photos"><span class="yjm_has_none"><span class="yjm_title">Photos</span></span></a></span></li><li class="haschild item105 level0"><span class="child"><a class="yjanchor " href="https://2014.igem.org/Team:UMaryland/meetourteam"><span class="yjm_has_none"><span class="yjm_title">Team</span></span></a></span><ul class="subul_main level1"><li class="bl"></li><li class="tl"></li><li class="tr"></li><li class="item293 level1 first"><span class="mymarg"><a class="yjanchor  first" href="https://2014.igem.org/Team:UMaryland/meetourteam"><span class="yjm_has_none"><span class="yjm_title">Meet Our Team Members</span></span></a></span></li><li class="item116 level1"><span class="mymarg"><a class="yjanchor " href="https://igem.org/Team.cgi?id=1489"><span class="yjm_has_none"><span class="yjm_title">iGEM Page</span></span></a></span></li><li class="item117 level1"><span class="mymarg"><a class="yjanchor " href="https://2014.igem.org/Team:UMaryland/team/attributions"><span class="yjm_has_none"><span class="yjm_title">Attributions</span></span></a></span></li><li class="item300 level1 lilast"><span class="mymarg"><a class="yjanchor last" href="https://2014.igem.org/Team:UMaryland/team/sponsors"><span class="yjm_has_none"><span class="yjm_title">Sponsors</span></span></a></span></li><li class="right"></li><li class="br"></li></ul></li><li class=" active haschild item112 level0"><span class="child"><a class="yjanchor  activepath yjscroll" href="https://2014.igem.org/Team:UMaryland/project/overview"><span class="yjm_has_none"><span class="yjm_title">Project</span></span></a></span><ul class="subul_main level1"><li class="bl"></li><li class="tl"></li><li class="tr"></li><li class="item138 level1 first"><span class="mymarg"><a class="yjanchor  first" href="https://2014.igem.org/Team:UMaryland/project/overview"><span class="yjm_has_none"><span class="yjm_title">Overview</span></span></a></span></li><li class="item145 level1"><span class="mymarg"><a class="yjanchor " href="https://2014.igem.org/Team:UMaryland/project/background"><span class="yjm_has_none"><span class="yjm_title">Background</span></span></a></span></li><li id="current" class=" active item144 level1"><span class="mymarg"><a class="yjanchor  activepath " href="https://2014.igem.org/Team:UMaryland/project/biobricks "><span class="yjm_has_none"><span class="yjm_title">parts (BioBricks)</span></span></a></span></li><li class="item102 level1"><a class="yjanchor " href="https://2014.igem.org/Team:UMaryland/project/results" target="_blank" ><span class="yjm_has_none"><span class="yjm_title">Results</span></span></a></li><li class="item254 level1"><span class="mymarg"><a class="yjanchor " href="https://2014.igem.org/Team:UMaryland/project/futureapplications"><span class="yjm_has_none"><span class="yjm_title">future applications</span></span></a></span></li><li class="item301 level1 lilast"><span class="mymarg"><a class="yjanchor last" href="https://igem.org/Safety/Safety_Form?team_id=1489"><span class="yjm_has_none"><span class="yjm_title">Safety</span></span></a></span></li><li class="right"></li><li class="br"></li></ul></li><li class="haschild item234 level0"><span class="child"><a class="yjanchor " href="https://2014.igem.org/Team:UMaryland/notebook"><span class="yjm_has_none"><span class="yjm_title">Notebook</span></span></a></span><ul class="subul_main level1"><li class="bl"></li><li class="tl"></li><li class="tr"></li><li class="item303 level1 first lilast"><span class="mymarg"><a class="yjanchor  firstlast" href="https://2014.igem.org/Team:UMaryland/notebook"><span class="yjm_has_none"><span class="yjm_title">Wetlab Notebook</span></span></a></span></li><li class="right"></li><li class="br"></li></ul></li><li class="haschild item123 level0"><span class="child"><a class="yjanchor " href="https://2014.igem.org/Team:UMaryland/humanprac/overview"><span class="yjm_has_none"><span class="yjm_title">Human Practices</span></span></a></span><ul class="subul_main level1"><li class="bl"></li><li class="tl"></li><li class="tr"></li><li class="item106 level1 first"><span class="mymarg"><a class="yjanchor  first" href="https://2014.igem.org/Team:UMaryland/humanprac/overview"><span class="yjm_has_none"><span class="yjm_title">Overview | Mission Statement</span></span></a></span></li><li class="haschild item129 level1"><span class="child subparent"><a class="yjanchor  grouptitle" href="https://2014.igem.org/Team:UMaryland/humanprac/publicschool"><span class="yjm_has_none"><span class="yjm_title">Public School Outreach</span></span></a></span><ul class="subul_main level2"><li class="bl"></li><li class="tl"></li><li class="tr"></li><li class="item134 level2 first lilast"><span class="mymarg"><a class="yjanchor  firstlast" href="https://2014.igem.org/Team:UMaryland/humanprac/publicschool"><span class="yjm_has_none"><span class="yjm_title">HighSchool</span></span></a></span></li><li class="right"></li><li class="br"></li></ul></li><li class="item131 level1"><span class="mymarg"><a class="yjanchor " href="https://2014.igem.org/Team:UMaryland/humanprac/community"><span class="yjm_has_none"><span class="yjm_title">Community Interaction</span></span></a></span></li><li class="item132 level1 lilast"><span class="mymarg"><a class="yjanchor last" href="https://2014.igem.org/Team:UMaryland/humanprac/publicawareness"><span class="yjm_has_none"><span class="yjm_title">Public Awareness</span></span></a></span></li><li class="right"></li><li class="br"></li></ul></li></ul></div>
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<p style="text-align: center;"><span style="color: #141823; font-family: Helvetica, Arial, 'lucida grande', tahoma, verdana, arial, sans-serif; font-size: 13px; line-height: 17.9400005340576px; white-space: pre-wrap;">A soluble his-tagged galectin from Bos taurus, expressed under Arabinose induction</span></p>
<p style="text-align: center;"><span style="color: #141823; font-family: Helvetica, Arial, 'lucida grande', tahoma, verdana, arial, sans-serif; font-size: 13px; line-height: 17.9400005340576px; white-space: pre-wrap;">A soluble his-tagged galectin from Bos taurus, expressed under Arabinose induction</span></p>
<p style="margin: 0.4em 0px 0.5em; line-height: 19.5px; color: #282828; font-family: arial, sans-serif; font-size: 13px; text-align: center;"><span style="font-size: 20px; font-weight: bold;">pBAD-RBS-Bovine Galectin-1-B0012 Terminator</span></p>
<p style="margin: 0.4em 0px 0.5em; line-height: 19.5px; color: #282828; font-family: arial, sans-serif; font-size: 13px; text-align: center;"><span style="font-size: 20px; font-weight: bold;">pBAD-RBS-Bovine Galectin-1-B0012 Terminator</span></p>
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<p style="margin: 0.4em 0px 0.5em; line-height: 19.5px; color: #282828; font-family: arial, sans-serif; font-size: 13px;">Soluble beta-galactoside binding protein (galectin) originally from Bos taurus. Believed to expressed both intracellularly and extracellularly, where they serve to binding various types of beta-galactosides and initialize signal cascades. Believed to be a dimer in its active form, as is true with most mammalian galectins.</p>
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<p style="margin: 0.4em 0px 0.5em; line-height: 19.5px; color: #282828; font-family: arial, sans-serif; font-size: 13px;">Soluble beta-galactoside binding protein (galectin) originally from Bos taurus. It is believed to expressed both intracellularly and extracellularly, where they serve to binding various types of beta-galactosides and initialize signal cascades. It is believed to be a dimer in its active form, as is true with most mammalian galectins.</p>
<p style="margin: 0.4em 0px 0.5em; line-height: 19.5px; color: #282828; font-family: arial, sans-serif; font-size: 13px;">This biobrick is under the pBAD promoter (BBa_K206000) with attached RBS (BBa_B0034), and utilizes an additional RNA polymerase terminator (BBa_B0012).</p>
<p style="margin: 0.4em 0px 0.5em; line-height: 19.5px; color: #282828; font-family: arial, sans-serif; font-size: 13px;">This biobrick is under the pBAD promoter (BBa_K206000) with attached RBS (BBa_B0034), and utilizes an additional RNA polymerase terminator (BBa_B0012).</p>
<p style="margin: 0.4em 0px 0.5em; line-height: 19.5px; color: #282828; font-family: arial, sans-serif; font-size: 13px;">UMaryland 2014 is interested in utilizing this part to investigate whether E. coli can recognize and bind to surface carbohydrates on a marine pathogen. Other uses of this part lie in the recognition of various carbohydrate ligands and potential activation of signal transduction pathways.</p>
<p style="margin: 0.4em 0px 0.5em; line-height: 19.5px; color: #282828; font-family: arial, sans-serif; font-size: 13px;">UMaryland 2014 is interested in utilizing this part to investigate whether E. coli can recognize and bind to surface carbohydrates on a marine pathogen. Other uses of this part lie in the recognition of various carbohydrate ligands and potential activation of signal transduction pathways.</p>
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<p style="text-align: center;"><span style="color: #141823; font-family: Helvetica, Arial, 'lucida grande', tahoma, verdana, arial, sans-serif; font-size: small;"><span style="line-height: 17.9400005340576px; white-space: pre-wrap;">This is the Bovine Galectin which should interact with beta-galactosides and intialize a signal cascase</span></span></p>
<p style="text-align: center;"><span style="color: #141823; font-family: Helvetica, Arial, 'lucida grande', tahoma, verdana, arial, sans-serif; font-size: small;"><span style="line-height: 17.9400005340576px; white-space: pre-wrap;">This is the Bovine Galectin which should interact with beta-galactosides and intialize a signal cascase</span></span></p>
<p style="text-align: center;"><span style="color: #282828; font-family: arial, sans-serif; font-size: 20px; font-weight: bold; line-height: 19.5px; text-align: start;">Bovine Galectin-1</span></p>
<p style="text-align: center;"><span style="color: #282828; font-family: arial, sans-serif; font-size: 20px; font-weight: bold; line-height: 19.5px; text-align: start;">Bovine Galectin-1</span></p>
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<p style="margin: 0.4em 0px 0.5em; line-height: 19.5px; color: #282828; font-family: arial, sans-serif; font-size: 13px;">Soluble beta-galactoside binding protein (galectin) originally from Bos taurus. Believed to expressed both intracellularly and extracellularly, where they serve to binding various types of beta-galactosides and initialize signal cascades. Believed to be a dimer in its active form, as is true with most mammalian galectins.</p>
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<p style="margin: 0.4em 0px 0.5em; line-height: 19.5px; color: #282828; font-family: arial, sans-serif; font-size: 13px;">Soluble beta-galactoside binding protein (galectin) originally from Bos taurus. It is believed to expressed both intracellularly and extracellularly, where they serve to binding various types of beta-galactosides and initialize signal cascades. It is believed to be a dimer in its active form, as is true with most mammalian galectins.</p>
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<p style="margin: 0.4em 0px 0.5em; line-height: 19.5px; color: #282828; font-family: arial, sans-serif; font-size: 13px;">UMaryland 2014 is interested in utilizing this part to investigate whether E. coli can recognize and bind to surface carbohydrates on a marine pathogen. Other uses of this part lie in the recognition of various carbohydrate ligands and potential activation of signal transduction pathways.</p> <div class="jb_pagin">
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<p style="margin: 0.4em 0px 0.5em; line-height: 19.5px; color: #282828; font-family: arial, sans-serif; font-size: 13px;">UMaryland 2014 is interested in utilizing this part to investigate whether E. coli can recognize and bind to surface carbohydrates on a marine pathogen. Other uses of this part lie in the recognition of various carbohydrate ligands and potential activation of signal transduction pathways.</p>
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<p style="margin: 0.4em 0px 0.5em; text-align: center;"><strong><span style="color: #282828; font-family: arial, sans-serif; font-size: small;"><span style="line-height: 19.5px;">Part:BBa_K1489005: </span></span><span style="text-align: left; line-height: 19.5px;"><span style="color: #282828; font-family: arial, sans-serif; font-size: small;">Crasseoterra virginica Galectin 1 (CvGal1)</span></span></strong></p>
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<p style="margin: 0.4em 0px 0.5em; line-height: 19.5px; color: #282828; font-family: arial, sans-serif; font-size: 13px;"> </p>
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<a href="https://2014.igem.org/Team:UMaryland/project/biobricks /2-latest/143-results" rel="prev">&lt; Prev</a>
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<p style="margin: 0.4em 0px 0.5em; line-height: 19.5px; color: #282828; font-family: arial, sans-serif; font-size: 13px;"><img src="https://static.igem.org/mediawiki/parts/0/01/UM_PSB1C3_-_BBa_K1489005.png" border="0" width="515" height="335" style="color: #141823; font-family: Helvetica, Arial, 'lucida grande', tahoma, verdana, arial, sans-serif; font-size: small; line-height: 17.9400005340576px; text-align: center; white-space: pre-wrap; cursor: se-resize !important; display: block; border: 0px; margin-left: auto; margin-right: auto;" /></p>
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<p style="margin: 0.4em 0px 0.5em; line-height: 19.5px; color: #282828; font-family: arial, sans-serif; font-size: 13px; text-align: center;"><span style="font-size: 20px; font-weight: bold; text-align: start;">Crasseoterra virginica Galectin 1 (CvGal1)</span></p>
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<p style="margin: 0.4em 0px 0.5em; line-height: 19.5px; color: #282828; font-family: arial, sans-serif; font-size: 13px;"><span style="font-size: 13px;">His-tagged soluble beta-galactoside binding protein (galectin) originally from Crasseoterra virginica. CvGal1 is believed to expressed both intracellularly and extracellularly, where they serve to binding various types of n-acetylgalactosamines and initialize signal cascades. It is also believed to be a tetramer in its active form, as is true with most invertebrate galectins.</span></p>
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<a href="https://2014.igem.org/Team:UMaryland/project/biobricks /2-latest/141-background-motivation" rel="next">Next &gt;</a>
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<p style="margin: 0.4em 0px 0.5em; line-height: 19.5px; color: #282828; font-family: arial, sans-serif; font-size: 13px;"><span style="font-size: 13px;"> </span></p>
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<p style="margin: 0.4em 0px 0.5em; text-align: center;"><span style="text-decoration: underline;"><strong><span style="color: #282828; font-family: arial, sans-serif; font-size: small; text-decoration: underline;"><span style="line-height: 19.5px;">Part:BBa_K1489006: </span></span><span style="color: #282828; font-family: arial, sans-serif; font-size: small;"><span style="line-height: 19.5px;">OmpA-CvGal1</span></span></strong></span></p>
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<p style="margin: 0.4em 0px 0.5em; text-align: center;"><span style="text-decoration: underline;"><strong><span style="color: #282828; font-family: arial, sans-serif; font-size: small;"><span style="line-height: 19.5px;"> </span></span></strong></span></p>
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<p style="margin: 0.4em 0px 0.5em; line-height: 19.5px; color: #282828; font-family: arial, sans-serif; font-size: 13px;"><img src="https://static.igem.org/mediawiki/parts/2/28/UM_PSB1C3_-_BBa_K1489006.png" border="0" width="515" height="335" style="font-size: small; color: #141823; font-family: Helvetica, Arial, 'lucida grande', tahoma, verdana, arial, sans-serif; line-height: 17.9400005340576px; text-align: center; white-space: pre-wrap; display: block; cursor: se-resize !important; border: 0px; margin-left: auto; margin-right: auto;" /></p>
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<p style="margin: 0.4em 0px 0.5em; line-height: 19.5px; color: #282828; font-family: arial, sans-serif; font-size: 13px; text-align: center;"><span style="font-size: 20px; font-weight: bold; text-align: start;">OmpA-CvGal1</span></p>
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<p style="margin: 0.4em 0px 0.5em; line-height: 19.5px; color: #282828; font-family: arial, sans-serif; font-size: 13px; text-align: center;"><span style="font-size: 20px; font-weight: bold; text-align: start;"> </span></p>
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<p style="margin: 0.4em 0px 0.5em; line-height: 19.5px; color: #282828; font-family: arial, sans-serif; font-size: 13px; text-align: left;"><span style="font-size: 13px;">CvGal1 fused to OmpA in order to anchor it in the membrane.</span></p>
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<li class="active"><span class="divider"><span class="icon-yjsg-marker addtips" title="You are here: "></span></span></li><li itemscope itemtype="http://data-vocabulary.org/Breadcrumb"><a href="/iGemWiki/" class="pathway" itemprop="url"><span itemprop="title">Home</span></a><span class="icon-yjsg-pathway"></span></li><li itemscope itemtype="http://data-vocabulary.org/Breadcrumb"><a href="https://2014.igem.org/Team:UMaryland/project/overview" class="pathway" itemprop="url"><span itemprop="title">Project</span></a></li><li itemscope itemtype="http://data-vocabulary.org/Breadcrumb"><span class="icon-yjsg-pathway"></span><span itemprop="title">parts (BioBricks)</span></li></ul>
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<div class="yjsg7_before"><div id="yjsg7" class="yjsg_grid yjsgsitew"><div id="user23" class="yjsgxhtml only_mod"><div class="yjsquare modid117"><div class="h2_holder"><h2 class="module_title"><span class="title_split titlesplit0">About</span> <span class="title_split titlesplit1">Umaryland</span></h2></div><div class="yjsquare_in"><p>UMaryland 2014 is the inaugural iGEM team of the University of Maryland, College Park. We are a combined effort of several departments and numerous faculty mentors. Although it is only our first year, we believe our hard work and dedication has paid off. We can't wait for this year's competition! GO TERPS!</p></div></div></div></div></div> <div class="footer_holders footer"><!-- footer -->
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Latest revision as of 20:40, 17 October 2014

Parts (BioBricks)

About Umaryland

UMaryland 2014 is the inaugural iGEM team of the University of Maryland, College Park. We are a combined effort of several departments and numerous faculty mentors. Although it is only our first year, we believe our hard work and dedication has paid off. We can't wait for this year's competition! GO TERPS!