Team:UMaryland/notebook

From 2014.igem.org

(Difference between revisions)
Line 161: Line 161:
<p class="MsoNormal">These are our two main project BioBricks: the Bovine galectin alone in pSB1C3-pBAD-RBS (BBa_K1489000) and the Bovine galectin gene fused to the end of the OmpA gene in the same vector (BBa_K1489004). Additionally, since the pSB1C3 backbone has a SacI site (which pSB1A2 did not) and OmpA has a SacI site (as designed by the creators of that BioBrick), the pSB1C3-OmpA which we created back in June had two unusable SacI sites. In order to maintain the integrity of that BioBrick with its unique restriction sites (along with the standard EcoRI and PstI sites), we also performed SDM on the pSB1C3-OmpA (BBa_K1489002) to replace the now-useless SacI site at the end of the OmpA gene with a KasI site, creating our fourth BioBrick (BBa_K1489003).</p>
<p class="MsoNormal">These are our two main project BioBricks: the Bovine galectin alone in pSB1C3-pBAD-RBS (BBa_K1489000) and the Bovine galectin gene fused to the end of the OmpA gene in the same vector (BBa_K1489004). Additionally, since the pSB1C3 backbone has a SacI site (which pSB1A2 did not) and OmpA has a SacI site (as designed by the creators of that BioBrick), the pSB1C3-OmpA which we created back in June had two unusable SacI sites. In order to maintain the integrity of that BioBrick with its unique restriction sites (along with the standard EcoRI and PstI sites), we also performed SDM on the pSB1C3-OmpA (BBa_K1489002) to replace the now-useless SacI site at the end of the OmpA gene with a KasI site, creating our fourth BioBrick (BBa_K1489003).</p>
<p class="MsoNormal"><strong>October</strong></p>
<p class="MsoNormal"><strong>October</strong></p>
-
<p class="MsoNormal">fillertext</p> <div class="jb_pagin">
+
<p class="MsoNormal">fillertext</p>
-
<ul class="pager">
+
-
<li class="next">
+
-
<a href="http://2014.igem.org/Team:UMaryland/notebook/2-latest/150-photo-album" rel="next">Next &gt;</a>
+
-
</li>
+
-
</ul></div>
+
-
</div>
+
</div>
</div>

Revision as of 21:06, 16 October 2014

Wetlab Notebook