Team:ULB-Brussels/Parts

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     Université Libre de Bruxelles  **/                -->
     Université Libre de Bruxelles  **/                -->
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<center><groupparts>iGEM14 ULB-Brussels</groupparts></center>
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<br/><br/><center><groupparts>iGEM14 ULB-Brussels</groupparts></center>
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$The$ $Mighty$ $Coli$ $BioBrick$
 
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<h1> Our Part </h1>
 
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$\Longrightarrow$ <a href="http://parts.igem.org/Part:BBa_K1318000"> ULB-Brussels part </a>
 
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<h3> Properties </h3>
 
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<p> Name: BBa_K1318000 <br>
 
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Host: E.coli <br>
 
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Source: ccdBA operon <br>
 
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Plasmid: available using <i>LabGenius BioBrick Mapper</i> <br>
 
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Length: 309 bp <br>
 
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Sequence: (begin) atgcagttt ... atataataa (end) <br>
 
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Compatibility: RFC[10], RFC[12], RFC[21], RFC[23], RFC[25]. <br>
 
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<h3> Characterization </h3>
 
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<p> In order to characterize the ccdB biobrick, we sent the biobricks to sequencing and made a <i>screen of activity </i> for the protein ccdB. We did a killing assay, because of the toxic property of ccdB.<br><!--$\small killing$ $\small assay$--><br>
 
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We constructed 4 different colonies including one with the plasmid pKK233/ccdA, another with pBAD33/ccdB, a third with both, and a control colony. The ccdA gene encoded for a protein wich acts as an anti-toxin of ccdB.<br><!--$\small\hspace{0.08cm} 4$ $\small different$ $\small colonies\hspace{0.06cm}$ --><br>
 
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On the first media containing <i>IPTG</i> (inducing the pKK233’s expression) <i>and glucose</i> (repressing the pBAD's expression), each colony grew. That allowed us to control the non toxicity of ccdA.
 
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On the media containing both <i>IPTG and arabinose</i> (inducing the pBAD's expression), the strand with pBAD was killed and the strand with both ccdA $\small\&$ ccdB grew.<br><br>
 
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We made <i>dilutions</i> to assure that the cell concentration didn’t affect the toxicity or the anti-toxicity. </p>
 
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<a href="https://2014.igem.org/Team:ULB-Brussels/Parts"> < Previous Bricks </a>
 
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Revision as of 08:35, 12 October 2014

$~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ \newcommand{\MyColi}{{\small Mighty\hspace{0.12cm}Coli}} \newcommand{\Stabi}{\small Stabi}$ $\newcommand{\EColi}{\small E.coli} \newcommand{\SCere}{\small S.cerevisae}\\[0cm] ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ \newcommand{\PI}{\small PI}$ $\newcommand{\Igo}{\Large\mathcal{I}} \newcommand{\Tgo}{\Large\mathcal{T}} \newcommand{\Ogo}{\Large\mathcal{O}} ~$ Example of a hierarchical menu in CSS

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- Université Libre de Bruxelles -




Parts




<groupparts>iGEM14 ULB-Brussels</groupparts>